Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The s...Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C_ 18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15∶18∶66∶1) for ES +, acetonitrile-methanol-water (15∶18∶67) for ES -. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L·h -1 . The ion source temperature was 150 ℃. Results The stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity. Conclusion It was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.展开更多
目的利用响应面法优选气血双补酒的渗漉提取工艺。方法采用单因素试验结合Box-Behnken中心组合设计方法,对提取工艺中乙醇体积分数、溶剂倍量、渗漉速率3个因素进行考察,以复方中6个指标性成分(人参皂苷Rb1、毛蕊异黄酮葡萄糖苷、阿魏...目的利用响应面法优选气血双补酒的渗漉提取工艺。方法采用单因素试验结合Box-Behnken中心组合设计方法,对提取工艺中乙醇体积分数、溶剂倍量、渗漉速率3个因素进行考察,以复方中6个指标性成分(人参皂苷Rb1、毛蕊异黄酮葡萄糖苷、阿魏酸、二苯乙烯苷、芍药苷、橙皮苷)及浸出物的质量浓度的综合评分作为考察指标进行提取工艺优化研究。结果最佳提取工艺为以10倍量48%乙醇,1.4 m L/min的体积流量渗漉提取。结论响应面法优选气血双补酒提取工艺简便、合理可行,具有较高的精确度,可为工业化大生产提供科学的依据。展开更多
目的建立胶束电动毛细管色谱二极管阵列检测法（MEKC-DAD）同时测定不同产地及商品何首乌中二苯乙烯苷、大黄素、芦荟大黄素、大黄酸、大黄酚、大黄素甲醚、儿茶素含量的方法。方法选择胶束电动毛细管分离模式,用未涂渍标准熔融石英毛...目的建立胶束电动毛细管色谱二极管阵列检测法（MEKC-DAD）同时测定不同产地及商品何首乌中二苯乙烯苷、大黄素、芦荟大黄素、大黄酸、大黄酚、大黄素甲醚、儿茶素含量的方法。方法选择胶束电动毛细管分离模式,用未涂渍标准熔融石英毛细管（75μm×64.5 cm,有效长度56 cm）作为分离通道;背景电解质溶液：25 mmol·L-1硼砂-30 mmol·L-1SDS-10%乙腈（p H 9.60）;检测波长：254 nm;运行电压：25 k V;运行温度：24℃;压力进样：25 k Pa,5 s。结果何首乌中7种成分的浓度与峰面积的线性关系良好（r≥0.999 4）;加样回收率为99.164%~101.504%。用此法测定何首乌中7种指标成分的含量,结果满意。结论该方法简单、准确,重现性好,为何首乌商品药材或饮片的质量评价和控制提供科学的参考依据。展开更多
文摘Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C_ 18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15∶18∶66∶1) for ES +, acetonitrile-methanol-water (15∶18∶67) for ES -. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L·h -1 . The ion source temperature was 150 ℃. Results The stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity. Conclusion It was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.
文摘目的利用响应面法优选气血双补酒的渗漉提取工艺。方法采用单因素试验结合Box-Behnken中心组合设计方法,对提取工艺中乙醇体积分数、溶剂倍量、渗漉速率3个因素进行考察,以复方中6个指标性成分(人参皂苷Rb1、毛蕊异黄酮葡萄糖苷、阿魏酸、二苯乙烯苷、芍药苷、橙皮苷)及浸出物的质量浓度的综合评分作为考察指标进行提取工艺优化研究。结果最佳提取工艺为以10倍量48%乙醇,1.4 m L/min的体积流量渗漉提取。结论响应面法优选气血双补酒提取工艺简便、合理可行,具有较高的精确度,可为工业化大生产提供科学的依据。
文摘目的建立胶束电动毛细管色谱二极管阵列检测法（MEKC-DAD）同时测定不同产地及商品何首乌中二苯乙烯苷、大黄素、芦荟大黄素、大黄酸、大黄酚、大黄素甲醚、儿茶素含量的方法。方法选择胶束电动毛细管分离模式,用未涂渍标准熔融石英毛细管（75μm×64.5 cm,有效长度56 cm）作为分离通道;背景电解质溶液：25 mmol·L-1硼砂-30 mmol·L-1SDS-10%乙腈（p H 9.60）;检测波长：254 nm;运行电压：25 k V;运行温度：24℃;压力进样：25 k Pa,5 s。结果何首乌中7种成分的浓度与峰面积的线性关系良好（r≥0.999 4）;加样回收率为99.164%~101.504%。用此法测定何首乌中7种指标成分的含量,结果满意。结论该方法简单、准确,重现性好,为何首乌商品药材或饮片的质量评价和控制提供科学的参考依据。
