Using foxtail millet (Setaria italica (L.) Beauv.) male-sterile line 1066A as female parent and Yugu 1 primary trisomic series (1 - 7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male-sterili...Using foxtail millet (Setaria italica (L.) Beauv.) male-sterile line 1066A as female parent and Yugu 1 primary trisomic series (1 - 7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male-sterility and yellow seedling in line 1066A was studied by primary trisomic analysis. The plants of F-1 generation of trisomics 2 - 9 were obtained by crossing with a great many plants of 1066A. F-1 generation of trisomics was similar to their male parent in morphologic characters, the color of their seedling was green, and pollen was partially fertile. The segregation ratio of fertility to sterility is 3:1 in F-2 generation of trisomics 2, 3, 4, 5, 7, 8 and 9; and 14:1 only in F-2 generation of trisomic 6 (chi(0.05)(2) = 0.012). The segregation ratio of green seedling to yellow seedling is 12:1 only in F-2 generation of trisomic 7 (chi(0.05)(2) = 0.31), but in other cases, this ratio is 3:1. The results indicated that the male-sterility gene was located on chromosome 6, and the gene for yellow seedling was monogenic recessive and located on chromosome 7. The rate of trisomics transmission by pollen was tested, trisomics 8 and 9 were the highest in rates of trisomics transmission and followed by trisomics 6 and 4.展开更多
The technique of tissue culture for cereal improvement has attracted close attention of many scientists. But successful application in plant breeding and genetic manipulation requires not only the initiation and growt...The technique of tissue culture for cereal improvement has attracted close attention of many scientists. But successful application in plant breeding and genetic manipulation requires not only the initiation and growth of tissue calli from explants, but also subsequent plantlet regeneration. It is significant to abtain totipotent cultures展开更多
[目的]研究影响东方白果(Solanum texanum)种子无菌苗萌发的因素,为其组织培养奠定基础。[方法]采取不同的预处理和消毒方法处理种子,观察其萌发情况。[结果]用70℃水浸种10 m in,经75%乙醇消毒5 m in和0.1%HgC l2消毒30 s,放置于灭菌...[目的]研究影响东方白果(Solanum texanum)种子无菌苗萌发的因素,为其组织培养奠定基础。[方法]采取不同的预处理和消毒方法处理种子,观察其萌发情况。[结果]用70℃水浸种10 m in,经75%乙醇消毒5 m in和0.1%HgC l2消毒30 s,放置于灭菌的脱脂棉中培养,可在7 d内获得无菌苗,种子萌发率可达到75.0%。[结论]该方法适合东方白果种子无菌苗的萌发,也可为其他科属种皮坚硬种子的萌发提供依据。展开更多
文摘Using foxtail millet (Setaria italica (L.) Beauv.) male-sterile line 1066A as female parent and Yugu 1 primary trisomic series (1 - 7) and tetrasomics 8, 9 as male parents, chromosome location of gene for male-sterility and yellow seedling in line 1066A was studied by primary trisomic analysis. The plants of F-1 generation of trisomics 2 - 9 were obtained by crossing with a great many plants of 1066A. F-1 generation of trisomics was similar to their male parent in morphologic characters, the color of their seedling was green, and pollen was partially fertile. The segregation ratio of fertility to sterility is 3:1 in F-2 generation of trisomics 2, 3, 4, 5, 7, 8 and 9; and 14:1 only in F-2 generation of trisomic 6 (chi(0.05)(2) = 0.012). The segregation ratio of green seedling to yellow seedling is 12:1 only in F-2 generation of trisomic 7 (chi(0.05)(2) = 0.31), but in other cases, this ratio is 3:1. The results indicated that the male-sterility gene was located on chromosome 6, and the gene for yellow seedling was monogenic recessive and located on chromosome 7. The rate of trisomics transmission by pollen was tested, trisomics 8 and 9 were the highest in rates of trisomics transmission and followed by trisomics 6 and 4.
文摘The technique of tissue culture for cereal improvement has attracted close attention of many scientists. But successful application in plant breeding and genetic manipulation requires not only the initiation and growth of tissue calli from explants, but also subsequent plantlet regeneration. It is significant to abtain totipotent cultures
文摘[目的]研究影响东方白果(Solanum texanum)种子无菌苗萌发的因素,为其组织培养奠定基础。[方法]采取不同的预处理和消毒方法处理种子,观察其萌发情况。[结果]用70℃水浸种10 m in,经75%乙醇消毒5 m in和0.1%HgC l2消毒30 s,放置于灭菌的脱脂棉中培养,可在7 d内获得无菌苗,种子萌发率可达到75.0%。[结论]该方法适合东方白果种子无菌苗的萌发,也可为其他科属种皮坚硬种子的萌发提供依据。
