Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-ste...Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-steady state assumption.Effects of hydration temperature,product concentration and substrate concentration on NHase deactivation were investigated experimentally and correlated with a first order deactivation kinetics.The results showed that the hydration temperature and product concentration were major factors governing the deactivation of NHases under substrate-feeding conditions.When acrylamide concentration was higher than 250 g·L1,the deactivation of NHases became serious and the bi-steady state assumption was not applicable.When the hydration temperature was controlled at a relatively higher level such as 28°C,the total deactivation rate constant was about 2.8-fold of that at 20°C.展开更多
A classical particle system coupled with a thermostat driven by an external constant force reaches its steady state when the ensemble-averaged drift velocity does not vary with time.In this work,the statistical mechan...A classical particle system coupled with a thermostat driven by an external constant force reaches its steady state when the ensemble-averaged drift velocity does not vary with time.In this work,the statistical mechanics of such a system is derived solely based on the equiprobability and ergodicity principles,free from any conclusions drawn on equilibrium statistical mechanics or local equilibrium hypothesis.The momentum space distribution is determined by a random walk argument,and the position space distribution is determined by employing the equiprobability and ergodicity principles.The expressions for energy,entropy,free energy,and pressures are then deduced,and the relation among external force,drift velocity,and temperature is also established.Moreover,the relaxation towards its equilibrium is found to be an exponentially decaying process obeying the minimum entropy production theorem.展开更多
In this paper, a system of reaction-diffusion equations arising in ecoepidemiological systems is investigated. The equations model a situation in which a predator species and a prey species inhabit the same bounded re...In this paper, a system of reaction-diffusion equations arising in ecoepidemiological systems is investigated. The equations model a situation in which a predator species and a prey species inhabit the same bounded region and the predator only eats the prey with transmissible diseases. Local stability of the constant positive solution is considered. A number of existence and non-existence results about the nonconstant steady states of a reaction diffusion system are given. It is proved that if the diffusion coefficient of the prey with disease is treated as a bifurcation parameter, non-constant positive steady-state solutions may bifurcate from the constant steadystate solution under some conditions.展开更多
基金Supported by the Foundation for the Authors of National Excellent Doctoral Dissertation of China (200345)the National High Technology Research and Development Program of China (2007AA02Z201)the National Basic Research Program of China (2007CB714304)
文摘Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-steady state assumption.Effects of hydration temperature,product concentration and substrate concentration on NHase deactivation were investigated experimentally and correlated with a first order deactivation kinetics.The results showed that the hydration temperature and product concentration were major factors governing the deactivation of NHases under substrate-feeding conditions.When acrylamide concentration was higher than 250 g·L1,the deactivation of NHases became serious and the bi-steady state assumption was not applicable.When the hydration temperature was controlled at a relatively higher level such as 28°C,the total deactivation rate constant was about 2.8-fold of that at 20°C.
基金funded by the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA17010504)the National Natural Science Foundation of China(Nos.11774357,11947302)。
文摘A classical particle system coupled with a thermostat driven by an external constant force reaches its steady state when the ensemble-averaged drift velocity does not vary with time.In this work,the statistical mechanics of such a system is derived solely based on the equiprobability and ergodicity principles,free from any conclusions drawn on equilibrium statistical mechanics or local equilibrium hypothesis.The momentum space distribution is determined by a random walk argument,and the position space distribution is determined by employing the equiprobability and ergodicity principles.The expressions for energy,entropy,free energy,and pressures are then deduced,and the relation among external force,drift velocity,and temperature is also established.Moreover,the relaxation towards its equilibrium is found to be an exponentially decaying process obeying the minimum entropy production theorem.
基金The NSF (10771085) of Chinathe Key Lab of Symbolic Computation and Knowledge Engineering of Ministry of Educationthe 985 program of Jilin University
文摘In this paper, a system of reaction-diffusion equations arising in ecoepidemiological systems is investigated. The equations model a situation in which a predator species and a prey species inhabit the same bounded region and the predator only eats the prey with transmissible diseases. Local stability of the constant positive solution is considered. A number of existence and non-existence results about the nonconstant steady states of a reaction diffusion system are given. It is proved that if the diffusion coefficient of the prey with disease is treated as a bifurcation parameter, non-constant positive steady-state solutions may bifurcate from the constant steadystate solution under some conditions.