为了研究柠檬酸作为引发剂对甜菜单粒种引发的影响效果,并为甜菜种子包衣剂物料的选择提供依据。试验以甜菜单粒种1113×NY051为材料,不同浓度柠檬酸为引发剂,用直接测量法得到发芽势、发芽率等数据。结果表明,处理甜菜单粒种时,不...为了研究柠檬酸作为引发剂对甜菜单粒种引发的影响效果,并为甜菜种子包衣剂物料的选择提供依据。试验以甜菜单粒种1113×NY051为材料,不同浓度柠檬酸为引发剂,用直接测量法得到发芽势、发芽率等数据。结果表明,处理甜菜单粒种时,不同浓度柠檬酸和不同时间梯度的9个处理都能够有效地促进材料1113×NY051的发芽势、发芽率、发芽指数和活力指数,降低电导率。其中,5 mmol/L柠檬酸对甜菜种子1113×NY051在8、12、24 h 3个不同时间情况下的发芽势、发芽率、发芽指数和活力指数指标均高出对照组(CK),电导率低于对照组(CK)。不同浓度的柠檬酸能显著促进种子萌发,随着处理时间的变化和试剂浓度的不同对引发效果均有不同影响。展开更多
谷子[Setaria italica (L.) P.Beauv]是起源于中国的一种古老作物,营养价值高,富含人体所需的多种维生素、氨基酸和矿物质,具有抗旱、耐瘠薄、抗逆性强的特点,尤其适合在北方干旱和半干旱地区种植。杂种优势利用已经成为提高作物产量的...谷子[Setaria italica (L.) P.Beauv]是起源于中国的一种古老作物,营养价值高,富含人体所需的多种维生素、氨基酸和矿物质,具有抗旱、耐瘠薄、抗逆性强的特点,尤其适合在北方干旱和半干旱地区种植。杂种优势利用已经成为提高作物产量的有效途径,在谷子杂交种应用方面已经获得了一定突破。谷子杂交种是由不育系母本和恢复系父本配组育成的两系杂交种,且母本具有5%左右的自交结实率,因此在制种过程中不可避免地会导致杂交种中混杂一定的不育系自交种。此外,由于授粉时杂株花粉的污染、收获时的机械混杂以及收获后的处理程序等原因,也会导致杂交种中混有恢复系和其他假杂交种,从而严重影响谷子杂交种的纯度。这些假杂种或者几乎没有产量,或者产量明显低于真杂交种,它们的存在必然导致杂交种增产潜力得不到充分发挥。因此,如何有效地鉴定谷子杂交种的纯度,成为保证杂交种增产效果的重要一环。展开更多
Use of a single seed is very useful for genetic studies on Vitis vinifera. However, molecular markers require a fair amount of high purity DNA. Grapevine contains high concentrations of polysaccharides, polyphenols, t...Use of a single seed is very useful for genetic studies on Vitis vinifera. However, molecular markers require a fair amount of high purity DNA. Grapevine contains high concentrations of polysaccharides, polyphenols, tannins and other secondary metabolites. These compounds may hamper the DNA isolation processes and subsequent analysis. In this study we have compared two DNA isolation methods: the NucleoSpin Plant II method and a modified protocol from Doyle and Doyle. The average value of 260/280 nm absorption ratio, which is used to assess the purity of DNA and RNA was 1.8 (accepted as “pure” DNA) and 0.9 (presence of protein or other contaminants) for the first and second method, respectively. Using the NucleoSpin protocol, from a single seed (20 - 35 mg) we obtained an average yield of extracted DNA of 24.8 ± 5.2 to 38.4 ± 11.5 ng·mg-1 dry weight. Among the two protocols examined, the NucleoSpin method was more efficient and gave better quality of DNA values compared to those from the modified Doyle and Doyle procedures.展开更多
文摘为了研究柠檬酸作为引发剂对甜菜单粒种引发的影响效果,并为甜菜种子包衣剂物料的选择提供依据。试验以甜菜单粒种1113×NY051为材料,不同浓度柠檬酸为引发剂,用直接测量法得到发芽势、发芽率等数据。结果表明,处理甜菜单粒种时,不同浓度柠檬酸和不同时间梯度的9个处理都能够有效地促进材料1113×NY051的发芽势、发芽率、发芽指数和活力指数,降低电导率。其中,5 mmol/L柠檬酸对甜菜种子1113×NY051在8、12、24 h 3个不同时间情况下的发芽势、发芽率、发芽指数和活力指数指标均高出对照组(CK),电导率低于对照组(CK)。不同浓度的柠檬酸能显著促进种子萌发,随着处理时间的变化和试剂浓度的不同对引发效果均有不同影响。
文摘谷子[Setaria italica (L.) P.Beauv]是起源于中国的一种古老作物,营养价值高,富含人体所需的多种维生素、氨基酸和矿物质,具有抗旱、耐瘠薄、抗逆性强的特点,尤其适合在北方干旱和半干旱地区种植。杂种优势利用已经成为提高作物产量的有效途径,在谷子杂交种应用方面已经获得了一定突破。谷子杂交种是由不育系母本和恢复系父本配组育成的两系杂交种,且母本具有5%左右的自交结实率,因此在制种过程中不可避免地会导致杂交种中混杂一定的不育系自交种。此外,由于授粉时杂株花粉的污染、收获时的机械混杂以及收获后的处理程序等原因,也会导致杂交种中混有恢复系和其他假杂交种,从而严重影响谷子杂交种的纯度。这些假杂种或者几乎没有产量,或者产量明显低于真杂交种,它们的存在必然导致杂交种增产潜力得不到充分发挥。因此,如何有效地鉴定谷子杂交种的纯度,成为保证杂交种增产效果的重要一环。
文摘Use of a single seed is very useful for genetic studies on Vitis vinifera. However, molecular markers require a fair amount of high purity DNA. Grapevine contains high concentrations of polysaccharides, polyphenols, tannins and other secondary metabolites. These compounds may hamper the DNA isolation processes and subsequent analysis. In this study we have compared two DNA isolation methods: the NucleoSpin Plant II method and a modified protocol from Doyle and Doyle. The average value of 260/280 nm absorption ratio, which is used to assess the purity of DNA and RNA was 1.8 (accepted as “pure” DNA) and 0.9 (presence of protein or other contaminants) for the first and second method, respectively. Using the NucleoSpin protocol, from a single seed (20 - 35 mg) we obtained an average yield of extracted DNA of 24.8 ± 5.2 to 38.4 ± 11.5 ng·mg-1 dry weight. Among the two protocols examined, the NucleoSpin method was more efficient and gave better quality of DNA values compared to those from the modified Doyle and Doyle procedures.
