Objective To control stem blight disease of Schizonepeta tenuifolia caused by Phytophthora nicotianae. Methods The antagonist effect of 13 Trichoderma strains (including T. viride and T. harzianum) was evaluated upon ...Objective To control stem blight disease of Schizonepeta tenuifolia caused by Phytophthora nicotianae. Methods The antagonist effect of 13 Trichoderma strains (including T. viride and T. harzianum) was evaluated upon mycelia growth of P. nicotianae. Trichoderma strains with high antagonistic activities against the pathogen were used to control stem blight of S. tenuifolia in the field. Results Of 13 Trichoderma strains tested, T. viride strain M3 showed maximum mycelia growth inhibition (83.2%) to the pathogen, followed by T. viride strain Tv04-2 (78.2%) and then T. harzianum strain ThB (65.0%), in vitro. Fungal cell wall degrading enzymes, protease, and β-1,3-glucanase were analyzed qualitatively and quantitatively in further study. T. viride strains M3, Tv04-2, and T. harzianum strain ThB efficiently against P. nicotianae were used to control stem blight of S. tenuifolia in the field, and T. viride strain M3 showed the best biocontrol potential. Conclusion Trichoderma spp. can be used as alternatives of pesticides to control stem blight, one of the serious soilborne diseases of S. tenuifolia caused by P. nicotianae. However, though T. viride strains Tv04-2 and T. harzianum strain ThB are also highly against P. nicotianae in vitro, the controlling efficacy of them on stem blight disease is not as excellent as T. viride strains M3 in the field.展开更多
目的观察替硝唑口服联合荆芥水煎液外用治疗阴道滴虫病的疗效,并探讨其对患者血清白介素(IL)-2、IL-4和IL-10水平的影响。方法平行设计选取2013年1月至2016年1月铜川市人民医院南院和西安交通大学第一附属医院妇产科符合阴道滴虫病的患...目的观察替硝唑口服联合荆芥水煎液外用治疗阴道滴虫病的疗效,并探讨其对患者血清白介素(IL)-2、IL-4和IL-10水平的影响。方法平行设计选取2013年1月至2016年1月铜川市人民医院南院和西安交通大学第一附属医院妇产科符合阴道滴虫病的患者随机分为对照组和观察组,每组60例。对照组予替硝唑口服治疗,观察组在对照组治疗的基础上予荆芥水煎液阴道外用,治疗14 d。观察两组患者临床症状和体征改善的所需时间;显微镜检查阴道分泌物悬液;酶联免疫法检测患者治疗前后的血清IL-2、IL-4和IL-10水平;随访3个月以观察复发情况。结果观察组患者的总有效率为90.0%(55/60),明显高于对照组的73.3%(44/60),差异有统计学意义(P<0.05);两组患者治疗前后血清IL-2、IL-4和IL-10浓度水平比较,差异均有统计学意义(P<0.05);观察组治疗前后血清IL-2、IL-10浓度差值明显多于对照组[(6.5±2.7)pg/m L vs(3.6±1.7)pg/m L,(6.8±2.6)pg/m L vs(2.6±0.9)pg/m L],差异均有统计学意义(P<0.05),而观察组治疗前后血清IL-4浓度差值稍多于对照组,但差异无统计学意义(P>0.05);随访3个月,对照组复发率为18.3%(4/60),明显高于观察组的6.7%(11/60),差异有显著统计学意义(P<0.01)。结论替硝唑口服联合荆芥水煎液外用能够治疗阴道滴虫病,症状改善与复发率方面明显优于单一使用抗滴虫药物,其可能通过抗滴虫治疗与局部调节炎症反应从而提高疗效。展开更多
基金Central Public-interest Scientific Institution Basal Research Fund (yz-08-09)
文摘Objective To control stem blight disease of Schizonepeta tenuifolia caused by Phytophthora nicotianae. Methods The antagonist effect of 13 Trichoderma strains (including T. viride and T. harzianum) was evaluated upon mycelia growth of P. nicotianae. Trichoderma strains with high antagonistic activities against the pathogen were used to control stem blight of S. tenuifolia in the field. Results Of 13 Trichoderma strains tested, T. viride strain M3 showed maximum mycelia growth inhibition (83.2%) to the pathogen, followed by T. viride strain Tv04-2 (78.2%) and then T. harzianum strain ThB (65.0%), in vitro. Fungal cell wall degrading enzymes, protease, and β-1,3-glucanase were analyzed qualitatively and quantitatively in further study. T. viride strains M3, Tv04-2, and T. harzianum strain ThB efficiently against P. nicotianae were used to control stem blight of S. tenuifolia in the field, and T. viride strain M3 showed the best biocontrol potential. Conclusion Trichoderma spp. can be used as alternatives of pesticides to control stem blight, one of the serious soilborne diseases of S. tenuifolia caused by P. nicotianae. However, though T. viride strains Tv04-2 and T. harzianum strain ThB are also highly against P. nicotianae in vitro, the controlling efficacy of them on stem blight disease is not as excellent as T. viride strains M3 in the field.
文摘目的观察替硝唑口服联合荆芥水煎液外用治疗阴道滴虫病的疗效,并探讨其对患者血清白介素(IL)-2、IL-4和IL-10水平的影响。方法平行设计选取2013年1月至2016年1月铜川市人民医院南院和西安交通大学第一附属医院妇产科符合阴道滴虫病的患者随机分为对照组和观察组,每组60例。对照组予替硝唑口服治疗,观察组在对照组治疗的基础上予荆芥水煎液阴道外用,治疗14 d。观察两组患者临床症状和体征改善的所需时间;显微镜检查阴道分泌物悬液;酶联免疫法检测患者治疗前后的血清IL-2、IL-4和IL-10水平;随访3个月以观察复发情况。结果观察组患者的总有效率为90.0%(55/60),明显高于对照组的73.3%(44/60),差异有统计学意义(P<0.05);两组患者治疗前后血清IL-2、IL-4和IL-10浓度水平比较,差异均有统计学意义(P<0.05);观察组治疗前后血清IL-2、IL-10浓度差值明显多于对照组[(6.5±2.7)pg/m L vs(3.6±1.7)pg/m L,(6.8±2.6)pg/m L vs(2.6±0.9)pg/m L],差异均有统计学意义(P<0.05),而观察组治疗前后血清IL-4浓度差值稍多于对照组,但差异无统计学意义(P>0.05);随访3个月,对照组复发率为18.3%(4/60),明显高于观察组的6.7%(11/60),差异有显著统计学意义(P<0.01)。结论替硝唑口服联合荆芥水煎液外用能够治疗阴道滴虫病,症状改善与复发率方面明显优于单一使用抗滴虫药物,其可能通过抗滴虫治疗与局部调节炎症反应从而提高疗效。
