This paper studied the effects of cinnamon acid treatments on the respiratory rate and related enzymes activity in the seedling roots of Malus hupehensis Rehd.It would provide information for understanding the mechani...This paper studied the effects of cinnamon acid treatments on the respiratory rate and related enzymes activity in the seedling roots of Malus hupehensis Rehd.It would provide information for understanding the mechanisms of inhibition damage caused by continuous cultivation of apple tree.20 mL of solution containing different concentrations of cinnamon acid was added into container with the tested seedlings.After treatment,the samples were taken periodically and the respiratory rates were measured by OXY-LAB oxygen electrodes under 25°C stable temperature and then the activities of related enzymes were measured.The rates of total respiration and other 2 pathways [tricarboxylic acid cycle （TCA） and pentose phosphate pathway （PPP）] appeared initially an increasing treads and late （on the 3rd d） began to decline.However,they again appeared an increase trend at the end period,on the contrast,the respiratory rate of embden-meyer- hot-parnas （EMP） pathway appeared a stead decline tread but it had a recover on the last day.The respiratory rate of total and 3 pathways were decreased under 125 mg kg-1 （soil）.The dynamic trends of the enzymes activities of pyrophosphate-dependent phosphofructokinase （PFK）,glucose-6-phosphate dehydrogenase （G-6-PDH） and malate dehydrogenase （MDH） showed similarly.In conclusion,treatments of certain concentration of cinnamon acid would inhibit the respiratory rate and related enzymes activity of roots of M.hupehensis Rehd.And the inhibition degrees were positively related with concentration of cinnamon acid treatments.展开更多
Fishes exposed to different dissolved oxygen concentrations over prolonged time periods have different susceptibility and adaptation mechanisms.In this study,healthy grass carp with an average weight of 214.6±18....Fishes exposed to different dissolved oxygen concentrations over prolonged time periods have different susceptibility and adaptation mechanisms.In this study,healthy grass carp with an average weight of 214.6±18.7 g were selected for long-term culture in recirculating aquaculture systems at three different dissolved oxygen concentrations,i.e.,3.00 mg/L[(2.57±0.33)mg/L,DO_(2.57)),5.00 mg/L[(4.61±0.11)mg/L,DO_(4.61)],which was the control group,and 7.00 mg/L[(6.50±0.48)mg/L,DO_(6.50)].Blood,gill,muscle,and liver samples were collected after 60 days.Using hematoxylin-eosin(HE),alcian blue periodic acid Schiff(AB-PAS)staining,determination of respiratory metabolism enzyme activity,and fluorescent quantitative PCR,the adaptability of juvenile grass carp facing different dissolved oxygen levels were explored.The results showed that the respiration rate increased,and hemoconcentration and hemoglobin(Hb),electrolyte and pH changed at DO2.57.Furthermore,gill lamellae became thinner and longer,the distance between them increased,the mucus on them decreased and the interstitial cell mass decreased.In addition,the relative expression of apoptosis-related genes bcl-2 and bcl-xl in gill tissue decreased,while caspase-3 and bax increased.In muscles and liver tissues,lactate dehydrogenase(LDH)activity and lactic acid content(LA)increased,pyruvate dehydrogenase(PDH)activity decreased,and genes related to the respiratory metabolic enzymes PDH-A4 and PDH-E1α also changed accordingly.The oxygen consumption rate decreased significantly(P<0.05).At DO_(6.50),the distance between gill lamellae,numbers of red blood cells(RBC)and the content of hemoglobin decreased wheras the mucus on gill lamellae increased.The activity of PDH,the expression of PDH-E1a in liver and oxygen consumption rate increased.Different dissolved oxygen concentrations affected the respiratory behavior,gill tissue structure,blood transport capacity and tissue enzyme activity of juvenile grass carp.In summary,juvenile grass carp showed different adaptation strate展开更多
Background and aim Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for the current pandemic,can have multi-organ impact.Recent studies show that liver injury could be a manifestation ...Background and aim Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for the current pandemic,can have multi-organ impact.Recent studies show that liver injury could be a manifestation of the disease,and that liver disease could also be related to a worse prognosis.Our aim was to compare the characteristics of patients with severe coronavirus disease 2019(COVID-19)due to SARS-CoV-2 who required intubation versus stable hospitalized patients to identify the early biochemical predictive factors of a severe course of COVID-19 and subsequent requirement for intubation,specifically in Mexican.Methods This was an observational case-control study nested in a cohort study.Complete medical records of patients admitted for confirmed COVID-19 at a tertiary level center in Mexico City were reviewed.Clinical and biochemical data were collected,and the characteristics of patients who required invasive mechanical ventilation(IMV)(cases)were compared with stable hospitalized patients without ventilation(controls).Results We evaluated 166 patients with COVID-19 due to SARS-CoV-2 infection;114(68.7%)were men,the mean age was 50.6±13.3 years,and 27(16.3%)required IMV.The comparative analysis between cases and controls showed(respectively)significantly lower blood oxygen saturation(SpO_(2))(73.5±12.0%vs.83.0±6.8%,P<0.0001)and elevated alanine aminotransferase(ALT)(128(14–1123)IU/L vs.33(8–453)IU/L,P=0.003),aspartate aminotransferase(AST)(214(17–1247)vs.44(12–498)IU/L,P=0.001),lactic dehydrogenase(LDH)(764.6±401.9 IU/L vs.461.0±185.6 IU/L,P=0.001),and D-dimer(3463(524–34,227)ng/mL vs.829(152–41,923)ng/mL,P=0.003)concentrations.Patients in the cases group were older(58.6±12.7 years vs.49.1±12.8 years,P=0.001).Multivariate analysis showed that important factors at admission predicting the requirement for IMV during hospitalization for COVID-19 were AST≥250 IU/L(odds ratio(OR)=64.8,95%confidence interval(CI)7.5–560.3,P<0.0001)and D-dimer≥3500 ng/mL(OR=4.1,95%CI 1.2–13.7,P=0.02).展开更多
基金suppoted by the Project of 948 from Ministry of Agriculture of China (2006-G28)the Non-profit Research Foundation from Ministry of Agriculture of China (nyhyzx07-024)+1 种基金the Ear Marked Fund for Modern Agro-Industry Technology Research System, Chinathe Key Innovation Project for Agricultural Application Technology of Shandong Province, China.
文摘This paper studied the effects of cinnamon acid treatments on the respiratory rate and related enzymes activity in the seedling roots of Malus hupehensis Rehd.It would provide information for understanding the mechanisms of inhibition damage caused by continuous cultivation of apple tree.20 mL of solution containing different concentrations of cinnamon acid was added into container with the tested seedlings.After treatment,the samples were taken periodically and the respiratory rates were measured by OXY-LAB oxygen electrodes under 25°C stable temperature and then the activities of related enzymes were measured.The rates of total respiration and other 2 pathways [tricarboxylic acid cycle （TCA） and pentose phosphate pathway （PPP）] appeared initially an increasing treads and late （on the 3rd d） began to decline.However,they again appeared an increase trend at the end period,on the contrast,the respiratory rate of embden-meyer- hot-parnas （EMP） pathway appeared a stead decline tread but it had a recover on the last day.The respiratory rate of total and 3 pathways were decreased under 125 mg kg-1 （soil）.The dynamic trends of the enzymes activities of pyrophosphate-dependent phosphofructokinase （PFK）,glucose-6-phosphate dehydrogenase （G-6-PDH） and malate dehydrogenase （MDH） showed similarly.In conclusion,treatments of certain concentration of cinnamon acid would inhibit the respiratory rate and related enzymes activity of roots of M.hupehensis Rehd.And the inhibition degrees were positively related with concentration of cinnamon acid treatments.
基金supported by the earmarked fund for CARS(CARS-45)the Fundamental Research Funds for the Central Universities(No.2662022SCYJ001)the National Key R&D Program of China(No.2019YFD0900303).
文摘Fishes exposed to different dissolved oxygen concentrations over prolonged time periods have different susceptibility and adaptation mechanisms.In this study,healthy grass carp with an average weight of 214.6±18.7 g were selected for long-term culture in recirculating aquaculture systems at three different dissolved oxygen concentrations,i.e.,3.00 mg/L[(2.57±0.33)mg/L,DO_(2.57)),5.00 mg/L[(4.61±0.11)mg/L,DO_(4.61)],which was the control group,and 7.00 mg/L[(6.50±0.48)mg/L,DO_(6.50)].Blood,gill,muscle,and liver samples were collected after 60 days.Using hematoxylin-eosin(HE),alcian blue periodic acid Schiff(AB-PAS)staining,determination of respiratory metabolism enzyme activity,and fluorescent quantitative PCR,the adaptability of juvenile grass carp facing different dissolved oxygen levels were explored.The results showed that the respiration rate increased,and hemoconcentration and hemoglobin(Hb),electrolyte and pH changed at DO2.57.Furthermore,gill lamellae became thinner and longer,the distance between them increased,the mucus on them decreased and the interstitial cell mass decreased.In addition,the relative expression of apoptosis-related genes bcl-2 and bcl-xl in gill tissue decreased,while caspase-3 and bax increased.In muscles and liver tissues,lactate dehydrogenase(LDH)activity and lactic acid content(LA)increased,pyruvate dehydrogenase(PDH)activity decreased,and genes related to the respiratory metabolic enzymes PDH-A4 and PDH-E1α also changed accordingly.The oxygen consumption rate decreased significantly(P<0.05).At DO_(6.50),the distance between gill lamellae,numbers of red blood cells(RBC)and the content of hemoglobin decreased wheras the mucus on gill lamellae increased.The activity of PDH,the expression of PDH-E1a in liver and oxygen consumption rate increased.Different dissolved oxygen concentrations affected the respiratory behavior,gill tissue structure,blood transport capacity and tissue enzyme activity of juvenile grass carp.In summary,juvenile grass carp showed different adaptation strate
文摘Background and aim Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for the current pandemic,can have multi-organ impact.Recent studies show that liver injury could be a manifestation of the disease,and that liver disease could also be related to a worse prognosis.Our aim was to compare the characteristics of patients with severe coronavirus disease 2019(COVID-19)due to SARS-CoV-2 who required intubation versus stable hospitalized patients to identify the early biochemical predictive factors of a severe course of COVID-19 and subsequent requirement for intubation,specifically in Mexican.Methods This was an observational case-control study nested in a cohort study.Complete medical records of patients admitted for confirmed COVID-19 at a tertiary level center in Mexico City were reviewed.Clinical and biochemical data were collected,and the characteristics of patients who required invasive mechanical ventilation(IMV)(cases)were compared with stable hospitalized patients without ventilation(controls).Results We evaluated 166 patients with COVID-19 due to SARS-CoV-2 infection;114(68.7%)were men,the mean age was 50.6±13.3 years,and 27(16.3%)required IMV.The comparative analysis between cases and controls showed(respectively)significantly lower blood oxygen saturation(SpO_(2))(73.5±12.0%vs.83.0±6.8%,P<0.0001)and elevated alanine aminotransferase(ALT)(128(14–1123)IU/L vs.33(8–453)IU/L,P=0.003),aspartate aminotransferase(AST)(214(17–1247)vs.44(12–498)IU/L,P=0.001),lactic dehydrogenase(LDH)(764.6±401.9 IU/L vs.461.0±185.6 IU/L,P=0.001),and D-dimer(3463(524–34,227)ng/mL vs.829(152–41,923)ng/mL,P=0.003)concentrations.Patients in the cases group were older(58.6±12.7 years vs.49.1±12.8 years,P=0.001).Multivariate analysis showed that important factors at admission predicting the requirement for IMV during hospitalization for COVID-19 were AST≥250 IU/L(odds ratio(OR)=64.8,95%confidence interval(CI)7.5–560.3,P<0.0001)and D-dimer≥3500 ng/mL(OR=4.1,95%CI 1.2–13.7,P=0.02).
