摘要
目的观察没食子酸丙酯对大鼠缺血再灌注心肌线粒体损伤的保护。方法结扎大鼠冠状动30min再灌注20min,测定心肌线粒体中琥珀酸脱氢酶(SDH)、细胞色素氧化酶(CCO)、Ca2+·Mg2+-ATP酶、Ca2+-ATP酶、超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-PX)活性及细胞色素、磷脂和丙二醛含量。结果没食子酸丙酯(20和40mg/kg,结扎冠状动脉前60minip)能显著减轻缺血再灌注所致琥珀酸脱氢酶(SDH)、细胞色素氧化酶(CCO)、Ca2+·Mg2+-ATP酶、Ca2+-ATP酶、超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶活性的抑制(P<0.05),及细胞色素aa3、细胞色素C和膜磷脂含量的减少,并能抑制丙二醛含量的升高。结论没食子酸丙酯对缺血再灌注时心肌线粒体功能具有保护作用,其机制可能是通过提高对氧自由基的清除功能和抑制脂质过氧化。
[Objective] To study the effect of propyl gallate on myocardial mitochondria induced by ischemia/reperfusion in rats. [Methods] Ischemia]reperfusion injury was induced by coronary artery ligation for 30 min and reperfusion for 20 min. The activity of Ca^2＋-ATPase, Ca^2＋·Mg^2＋-ATPase, succinct dehydrogenase （SDH）, cytochrome oxidase（CCO）, SOD, GSH-PX, and the content of Cytaa3 and Cyt C, phospholipid （PL） and MDA in the myocardial mitochondria were measured. [Results] Treatment with propyl gallate significandy reduced the decreased activity of Ca^2＋-ATPase,Ca^2＋· Mg^2＋-ATPase,SDH, CCO,SOD and GSH-PX （P 〈0.05）,and the decreased content of Cyt C,Cyt aa3 and PL, and attenuated the increased content of MDA induced by ischemia/reperfusion. [Conclusion] Propyl Gallate has a protective effect on injury of myocardial mitochondria induced by ischemia/reperfusion in rats, and the effects of propyl gallate are related to scavenging oxygen free radicals and reducing lipid proxidation.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第14期2139-2142,共4页
China Journal of Modern Medicine
关键词
没食子酸丙酯
缺血再灌注
心脏
线粒体
腺苷三磷酸酶类
呼吸酶类
Propyl gallate
ischemia/reperfusion
heart
mitochondrion
adenosine triphosphatase
respiratory enzymes
