The elite rice cultivar Yuejingsimiao 2 （YJ2） is characterized by a high level of grain quality and yield, and resistance against Magnaporthe oryzae. YJ2 showed 100% resistance to four fungal populations collected f...The elite rice cultivar Yuejingsimiao 2 （YJ2） is characterized by a high level of grain quality and yield, and resistance against Magnaporthe oryzae. YJ2 showed 100% resistance to four fungal populations collected from Guangdong, Sichuan, Liaoning, and Heilongjiang Provinces, which is a higher frequency than that shown by the well-known resistance （R） gene donor culti- vats such as Sanhuangzhan 2 and 28zhan. Segregation analysis for resistance with F2 and F4 populations indicated the re- sistance of YJ2 was controlled by multiple genes that are dominant or recessive. The putative R genes of YJ2 were roughly tagged by SSR markers, located on chromosomes 2, 6, 8, and 12, in a hulked-segregant analysis using genome-wide selected SSR markers with F4 lines that segregated into 3 resistant （R）： 1 susceptible （S） or 1R：3S. The recessive R gene on chromosome 8 was further mapped to an interval ~1.9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F4 line that segregated into 1R：3S. Given that no major R gene was mapped to this inter- val, the novel R gene was designated as pi55（t）. Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare, two genes that encode a leucine-rich repeat-containing protein and heavy-metalassociated domain-containing protein, respectively, were suggested as the most likely candidates for pi55（t）.展开更多
Objective:Autosomal recessive bestrophinopathy(ARB),a retinal degenerative disease,is characterized by central visual loss,yellowish multifocal diffuse subretinal deposits,and a dramatic decrease in the light peak on ...Objective:Autosomal recessive bestrophinopathy(ARB),a retinal degenerative disease,is characterized by central visual loss,yellowish multifocal diffuse subretinal deposits,and a dramatic decrease in the light peak on electrooculogram.The potential pathogenic mechanism involves mutations in the BEST1 gene,which encodes Ca2+-activated Cl−channels in the retinal pigment epithelium(RPE),resulting in degeneration of RPE and photoreceptor.In this study,the complete clinical characteristics of two Chinese ARB families were summarized.Methods:Pacific Biosciences(PacBio)single-molecule real-time(SMRT)sequencing was performed on the probands to screen for disease-causing gene mutations,and Sanger sequencing was applied to validate variants in the patients and their family members.Results:Two novel mutations,c.202T>C(chr11:61722628,p.Y68H)and c.867+97G>A,in the BEST1 gene were identified in the two Chinese ARB families.The novel missense mutation BEST1 c.202T>C(p.Y68H)resulted in the substitution of tyrosine with histidine in the N-terminal region of transmembrane domain 2 of bestrophin-1.Another novel variant,BEST1 c.867+97G>A(chr11:61725867),located in intron 7,might be considered a regulatory variant that changes allele-specific binding affinity based on motifs of important transcriptional regulators.Conclusion:Our findings represent the first use of third-generation sequencing(TGS)to identify novel BEST1 mutations in patients with ARB,indicating that TGS can be a more accurate and efficient tool for identifying mutations in specific genes.The novel variants identified further broaden the mutation spectrum of BEST1 in the Chinese population.展开更多
[Objective] This study aimed to investigate the effect of mating factor recombination on the development of edible mushroom,and explore the possibility for using self-crossing to breed sporeless/low sporing mushroom s...[Objective] This study aimed to investigate the effect of mating factor recombination on the development of edible mushroom,and explore the possibility for using self-crossing to breed sporeless/low sporing mushroom strains.[Method] Selecting Agrocybe salicacola strain YAASM0711 as the self-crossing material,six self-crossed combinations were directionally constructed with single spore growth rate as an indicator,to carry out comprehensive analysis of the qualitative traits（color,deformity and growth characteristics）,quantitative traits（shape,yield,size and number of fruit bodies） and mating types of its self-crossed F1 progenies.[Result] After mating factor recombination,self-crossed single spore progenies showed serious genetic differentiation,the average mycelial growth rate and agronomic traits of inbred progenies were lower than those of the parents.The phenotype in fruiting body formation could be divided into normal fruiting and abnormal fruiting（including abnormal fruiting bodies,sporeless or low sporing fruiting bodies,no fruiting）.To be specific,18% self-crossed strains had superior agronomic traits to the parents,which can be used for selecting predominant strains from self-crossed progeny populations based on different breeding purposes.[Conclusion] The genes controlling mycelial growth rate of single spore were linked with mating factors.The mating factor-recombinant single spore strains could be selected according to the correlation between mating type and growth rate.The fruiting result of self-crossed strains indicated that the genes related to sporeless/low sporing development were recessive.Not only genetic materials harboring development-deficient genes in edible mushroom can be obtained by self-crossing,but also the sporeless/low sporing mutant strains with important application prospects can be bred.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. U1131003)the National Transgenic Research Projects(Grant Nos. 2009ZX08009-023 and 2011ZX08001-002)+1 种基金the Guangdong Provincial Natural Science Foundation (Grant No. 10151064001000008)the President Science Foundation of Guangdong Academy of Agricultural Sciences (Grant No. 201101)
文摘The elite rice cultivar Yuejingsimiao 2 （YJ2） is characterized by a high level of grain quality and yield, and resistance against Magnaporthe oryzae. YJ2 showed 100% resistance to four fungal populations collected from Guangdong, Sichuan, Liaoning, and Heilongjiang Provinces, which is a higher frequency than that shown by the well-known resistance （R） gene donor culti- vats such as Sanhuangzhan 2 and 28zhan. Segregation analysis for resistance with F2 and F4 populations indicated the re- sistance of YJ2 was controlled by multiple genes that are dominant or recessive. The putative R genes of YJ2 were roughly tagged by SSR markers, located on chromosomes 2, 6, 8, and 12, in a hulked-segregant analysis using genome-wide selected SSR markers with F4 lines that segregated into 3 resistant （R）： 1 susceptible （S） or 1R：3S. The recessive R gene on chromosome 8 was further mapped to an interval ~1.9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F4 line that segregated into 1R：3S. Given that no major R gene was mapped to this inter- val, the novel R gene was designated as pi55（t）. Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare, two genes that encode a leucine-rich repeat-containing protein and heavy-metalassociated domain-containing protein, respectively, were suggested as the most likely candidates for pi55（t）.
文摘Objective:Autosomal recessive bestrophinopathy(ARB),a retinal degenerative disease,is characterized by central visual loss,yellowish multifocal diffuse subretinal deposits,and a dramatic decrease in the light peak on electrooculogram.The potential pathogenic mechanism involves mutations in the BEST1 gene,which encodes Ca2+-activated Cl−channels in the retinal pigment epithelium(RPE),resulting in degeneration of RPE and photoreceptor.In this study,the complete clinical characteristics of two Chinese ARB families were summarized.Methods:Pacific Biosciences(PacBio)single-molecule real-time(SMRT)sequencing was performed on the probands to screen for disease-causing gene mutations,and Sanger sequencing was applied to validate variants in the patients and their family members.Results:Two novel mutations,c.202T>C(chr11:61722628,p.Y68H)and c.867+97G>A,in the BEST1 gene were identified in the two Chinese ARB families.The novel missense mutation BEST1 c.202T>C(p.Y68H)resulted in the substitution of tyrosine with histidine in the N-terminal region of transmembrane domain 2 of bestrophin-1.Another novel variant,BEST1 c.867+97G>A(chr11:61725867),located in intron 7,might be considered a regulatory variant that changes allele-specific binding affinity based on motifs of important transcriptional regulators.Conclusion:Our findings represent the first use of third-generation sequencing(TGS)to identify novel BEST1 mutations in patients with ARB,indicating that TGS can be a more accurate and efficient tool for identifying mutations in specific genes.The novel variants identified further broaden the mutation spectrum of BEST1 in the Chinese population.
基金Supported by China Agricultural Research System(CARS-24)Natural Science Foundation of Yunnan Province(2011FZ214)National Natural Science Foundation of China(31101591)~~
文摘[Objective] This study aimed to investigate the effect of mating factor recombination on the development of edible mushroom,and explore the possibility for using self-crossing to breed sporeless/low sporing mushroom strains.[Method] Selecting Agrocybe salicacola strain YAASM0711 as the self-crossing material,six self-crossed combinations were directionally constructed with single spore growth rate as an indicator,to carry out comprehensive analysis of the qualitative traits（color,deformity and growth characteristics）,quantitative traits（shape,yield,size and number of fruit bodies） and mating types of its self-crossed F1 progenies.[Result] After mating factor recombination,self-crossed single spore progenies showed serious genetic differentiation,the average mycelial growth rate and agronomic traits of inbred progenies were lower than those of the parents.The phenotype in fruiting body formation could be divided into normal fruiting and abnormal fruiting（including abnormal fruiting bodies,sporeless or low sporing fruiting bodies,no fruiting）.To be specific,18% self-crossed strains had superior agronomic traits to the parents,which can be used for selecting predominant strains from self-crossed progeny populations based on different breeding purposes.[Conclusion] The genes controlling mycelial growth rate of single spore were linked with mating factors.The mating factor-recombinant single spore strains could be selected according to the correlation between mating type and growth rate.The fruiting result of self-crossed strains indicated that the genes related to sporeless/low sporing development were recessive.Not only genetic materials harboring development-deficient genes in edible mushroom can be obtained by self-crossing,but also the sporeless/low sporing mutant strains with important application prospects can be bred.
