目的通过搜集原发性高血压患者、原发性高血压首次并发脑卒中患者既往可能存在的各种行为危险因素的暴露史,测量两组各因素的暴露比例,探索原发性高血压患者行为因素与其脑卒中发病的关联,推断哪些行为因素是高血压患者脑卒中发病的危...目的通过搜集原发性高血压患者、原发性高血压首次并发脑卒中患者既往可能存在的各种行为危险因素的暴露史,测量两组各因素的暴露比例,探索原发性高血压患者行为因素与其脑卒中发病的关联,推断哪些行为因素是高血压患者脑卒中发病的危险因素,为脑卒中的防治提供依据。方法采用健康促进生活方式量表Ⅱ(HPLPⅡ)对广州市某两家三甲医院的原发性高血压患者进行问卷调查,按年龄、性别、高血压病程成组匹配,采用非条件Logistic回归模型进行统计分析。结果脉压差>40 mm Hg、运动锻炼、吸烟、未按量服药、未定时排便进入了多因素Logistic回归模型,OR值分别为0.198〔95%CI(0.092,0.426)〕、7.515〔95%CI(2.117,26.683)〕、7.588〔95%CI(2.124,27.111)〕、12.814〔95%CI(3.096,53.040)〕、4.446〔95%CI(1.282,15.419)〕。结论运动锻炼是延缓原发性高血压脑卒中发病的保护因素;脉压差>40 mm Hg、吸烟、未按量服药、未定时排便是原发性高血压脑卒中发病的危险因素。展开更多
AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformati...AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT prima展开更多
文摘目的通过搜集原发性高血压患者、原发性高血压首次并发脑卒中患者既往可能存在的各种行为危险因素的暴露史,测量两组各因素的暴露比例,探索原发性高血压患者行为因素与其脑卒中发病的关联,推断哪些行为因素是高血压患者脑卒中发病的危险因素,为脑卒中的防治提供依据。方法采用健康促进生活方式量表Ⅱ(HPLPⅡ)对广州市某两家三甲医院的原发性高血压患者进行问卷调查,按年龄、性别、高血压病程成组匹配,采用非条件Logistic回归模型进行统计分析。结果脉压差>40 mm Hg、运动锻炼、吸烟、未按量服药、未定时排便进入了多因素Logistic回归模型,OR值分别为0.198〔95%CI(0.092,0.426)〕、7.515〔95%CI(2.117,26.683)〕、7.588〔95%CI(2.124,27.111)〕、12.814〔95%CI(3.096,53.040)〕、4.446〔95%CI(1.282,15.419)〕。结论运动锻炼是延缓原发性高血压脑卒中发病的保护因素;脉压差>40 mm Hg、吸烟、未按量服药、未定时排便是原发性高血压脑卒中发病的危险因素。
基金Supported by the Tianjin Key Medical Discipline(Specialty)Construction Project(No.TJYXZDXK-037A)Tianjin Medical University Eye Hospital。
文摘AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT prima
