Vector-borne plant diseases have significant ecological and economic impacts,affecting farm profitability and forest composition throughout the world.Bacterial vector-borne pathogens have evolved sophisticated strateg...Vector-borne plant diseases have significant ecological and economic impacts,affecting farm profitability and forest composition throughout the world.Bacterial vector-borne pathogens have evolved sophisticated strategies to interact with their hemipteran insect vectors and plant hosts.These pathogens reside in plant vascular tissue,and their study represents an excellent opportunity to uncover novel biological mechanisms regulating intracellular pathogenesis and to contribute to the control of some of the world's most invasive emerging diseases.In this perspective,we highlight recent advances and major unanswered questions in the realm of bacterial vector-borne disease,focusing on liberibacters,phytoplasmas,spiroplasmas,and Xylella fastidiosa.展开更多
In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detectio...In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L.展开更多
在枣疯植原体16S rDNA片段内设计并合成引物对DZ16SF-2/DZ16SR-2（扩增190 bp片段）,用重组质粒pMD18-DZ16S为模板建立标准曲线,并建立枣疯植原体实时荧光定量PCR（SYBR Green I）检测体系。溶解曲线分析显示：该引物特异性好,该体系对...在枣疯植原体16S rDNA片段内设计并合成引物对DZ16SF-2/DZ16SR-2（扩增190 bp片段）,用重组质粒pMD18-DZ16S为模板建立标准曲线,并建立枣疯植原体实时荧光定量PCR（SYBR Green I）检测体系。溶解曲线分析显示：该引物特异性好,该体系对质粒模板的检测灵敏度达102 copies/μL。利用该体系对不同月份、不同发病级别的嫁接接种枣树接穗样品进行植原体含量测定。通过对高抗品种‘星光’,中抗品种‘黑腰子枣’、‘嘎嘎枣’和‘葫芦枣’,感病品种‘月光枣’、‘马牙枣’、‘莲蓬子’、‘北流西冬枣’和高感品种‘大红袍枣’嫁接到发病‘冬枣’砧木上所表现出的不同病级、病情指数和病原浓度比较结果发现,表现无症状的样品中植原体的含量在每克鲜重10^5～10^6个,发病Ⅰ～Ⅱ级枝条中植原体浓度在10^6～10^7个/g,Ⅲ～Ⅳ级发病枝条含量在10^7～10^8个/g鲜重,而V级发病枝条达到每克鲜重109个,表明接种发病的接穗症状严重度与其体内植原体浓度积累密切相关。高抗枣疯病‘星光’品种嫁接接穗体内病原浓度明显低于感病品种,而且有较多‘星光’接穗嫁接的发病砧木症状有所减轻,植原体浓度也有所降低。本研究可为探索枣树品种的抗病机制、抗病性鉴定及枣疯病植原体的防治提供参考。展开更多
基金P.R.-C.,S.S.,and G.C.are supported by grants from the USDA CDRE(2019-70016-29796,2016-70016-24833)G.C.L.B.is supported by an INRAE Department of Plant Health and Environment(SPE)grant(VMP-ADAPT),grants from the University of Bordeaux,and SFR Integrative Biology and Ecology.M.H.and M.M.are supported by a grant from the USDA ARS CRIS(8062-22410-006-00-D)+1 种基金M.H.W.H.and S.A.H.were funded from the BBSRC Institute Strategy Program(BB/P012574/1)the John Innes Foundation.M.H.and M.M.are funded by USDA ARS project 8062-22410-006-00-D.R.P.P.A.was funded by the Pierce's Disease Research Program,California Department of Food and Agriculture.
文摘Vector-borne plant diseases have significant ecological and economic impacts,affecting farm profitability and forest composition throughout the world.Bacterial vector-borne pathogens have evolved sophisticated strategies to interact with their hemipteran insect vectors and plant hosts.These pathogens reside in plant vascular tissue,and their study represents an excellent opportunity to uncover novel biological mechanisms regulating intracellular pathogenesis and to contribute to the control of some of the world's most invasive emerging diseases.In this perspective,we highlight recent advances and major unanswered questions in the realm of bacterial vector-borne disease,focusing on liberibacters,phytoplasmas,spiroplasmas,and Xylella fastidiosa.
基金the financial support provided by project of Science and Technology Tackling Key Problems in Henan Province(Grant No.182102110430)the National Key Research and Development Program of China(Grant No.2016YFD0101300)by China Agricultural Research system(CARS-12).
文摘In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L.
