目的探究神经介入联合阿替普酶静脉溶栓对急性缺血性脑卒中(AIS)患者脑血流动力学及神经功能的影响,为患者的治疗提供参考。方法回顾性分析榆林市星元医院神经内二科2015年12月至2018年12月收治的102例AIS患者的临床诊治资料,按照患者...目的探究神经介入联合阿替普酶静脉溶栓对急性缺血性脑卒中(AIS)患者脑血流动力学及神经功能的影响,为患者的治疗提供参考。方法回顾性分析榆林市星元医院神经内二科2015年12月至2018年12月收治的102例AIS患者的临床诊治资料,按照患者治疗方式将其分为观察组50例和对照组52例。对照组患者采用阿替普酶静脉溶栓治疗,观察组在对照组治疗的基础上联合神经介入治疗,连续治疗3个月,比较患者治疗后的临床疗效、脑血流动力学各项指标水平以及治疗前后的神经功能缺损评分(NIHSS)。结果治疗后观察组患者的治疗总有效率为96.0%,明显高于对照组的86.5%,差异有统计学意义(P<0.05);治疗后,观察组和对照组患者的血流速度(Vmin)[(9.9±1.8) cm/s vs (9.1±1.5) cm/s]、最小血流量(Qmin)[(4.9±1.2) mL/s vs (4.2±1.3) mL/s]、动态阻抗(DR)[(483.5±156.2) Pa·s/mL vs (418.4±159.2) Pa·s/mL]比较,观察组明显高于对照组,脑血管外周阻力(R)[(1 825.3±251.5) Pa·s/mL vs 1 965.5±261.8 Pa·s/m]、临界压力(CP)[(6.2±1.6) kPa vs (6.9±1.6) kPa]比较,观察组明显低于对照组,差异均有统计学意义(P<0.05),而脉搏波速(Wv)[(20.9±3.9) m/s vs (20.8±4.1) m/s]比较差异无统计学意义(P>0.05);两组患者治疗前的NIHSS评分比较差异无统计学意义(P>0.05);治疗24 h和治疗3个月后,观察组患者的NIHSS评分分别为(16.4±3.1)分,(11.3±2.1)分,明显低于对照组的(19.5±3.6)分,(15.8±2.9)分,差异均有统计学意义(P<0.05)。结论神经介入联合阿替普酶静脉溶栓治疗急性缺血性脑卒中临床疗效显著,能明显改善患者的神经功能,值得推广应用。展开更多
Electroacupuncture has been widely used to treat cognitive impairment after cerebral ischemia,but the underlying mechanism has not yet been fully elucidated.Studies have shown that autophagy plays an important role in...Electroacupuncture has been widely used to treat cognitive impairment after cerebral ischemia,but the underlying mechanism has not yet been fully elucidated.Studies have shown that autophagy plays an important role in the formation and development of cognitive impairment,and the phosphoinositide 3-kinase(PI3K)/Akt signaling pathway plays an important role in autophagy regulation.To investigate the role played by the PI3K/Akt signaling pathway in the electroacupuncture treatment of cerebral ischemia/reperfusion rat models,we first established a rat model of cerebral ischemia/reperfusion through the occlusion of the middle cerebral artery using the suture method.Starting at 2 hours after modeling,electroacupuncture was delivered at the Shenting(GV24)and Baihui(GV20)acupoints,with a dilatational wave(1-20 Hz frequency,2 mA intensity,6 V peak voltage),for 30 minutes/day over 8 consecutive days.Our results showed that electroacupuncture reduced the infarct volume in a rat model of cerebral ischemia/reperfusion injury,increased the mRNA expression levels of the PI3K/Akt signaling pathwayrelated factors Beclin-1,mammalian target of rapamycin(mTOR),and PI3K,increased the protein expression levels of phosphorylated Akt,Beclin-1,PI3K,and mTOR in the ischemic cerebral cortex,and simultaneously reduced p53 mRNA and protein expression levels.In the Morris water maze test,the latency to find the hidden platform was significantly shortened among rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.In the spatial probe test,the number of times that a rat crossed the target quadrant was increased in rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.Electroacupuncture stimulation applied to the Shenting(GV24)and Baihui(GV20)acupoints activated the PI3K/Akt signaling pathway and improved rat learning and memory impairment.This study was approved by the Animal Ethics Committee of the First Affiliated Hospital of Henan Unive展开更多
文摘目的探究神经介入联合阿替普酶静脉溶栓对急性缺血性脑卒中(AIS)患者脑血流动力学及神经功能的影响,为患者的治疗提供参考。方法回顾性分析榆林市星元医院神经内二科2015年12月至2018年12月收治的102例AIS患者的临床诊治资料,按照患者治疗方式将其分为观察组50例和对照组52例。对照组患者采用阿替普酶静脉溶栓治疗,观察组在对照组治疗的基础上联合神经介入治疗,连续治疗3个月,比较患者治疗后的临床疗效、脑血流动力学各项指标水平以及治疗前后的神经功能缺损评分(NIHSS)。结果治疗后观察组患者的治疗总有效率为96.0%,明显高于对照组的86.5%,差异有统计学意义(P<0.05);治疗后,观察组和对照组患者的血流速度(Vmin)[(9.9±1.8) cm/s vs (9.1±1.5) cm/s]、最小血流量(Qmin)[(4.9±1.2) mL/s vs (4.2±1.3) mL/s]、动态阻抗(DR)[(483.5±156.2) Pa·s/mL vs (418.4±159.2) Pa·s/mL]比较,观察组明显高于对照组,脑血管外周阻力(R)[(1 825.3±251.5) Pa·s/mL vs 1 965.5±261.8 Pa·s/m]、临界压力(CP)[(6.2±1.6) kPa vs (6.9±1.6) kPa]比较,观察组明显低于对照组,差异均有统计学意义(P<0.05),而脉搏波速(Wv)[(20.9±3.9) m/s vs (20.8±4.1) m/s]比较差异无统计学意义(P>0.05);两组患者治疗前的NIHSS评分比较差异无统计学意义(P>0.05);治疗24 h和治疗3个月后,观察组患者的NIHSS评分分别为(16.4±3.1)分,(11.3±2.1)分,明显低于对照组的(19.5±3.6)分,(15.8±2.9)分,差异均有统计学意义(P<0.05)。结论神经介入联合阿替普酶静脉溶栓治疗急性缺血性脑卒中临床疗效显著,能明显改善患者的神经功能,值得推广应用。
基金supported by the National Natural Science Foundation of China,No.81574042the Traditional Chinese Medicine Special Research Projects of Henan Province of China,No.2018JDZX011(both to XDF).
文摘Electroacupuncture has been widely used to treat cognitive impairment after cerebral ischemia,but the underlying mechanism has not yet been fully elucidated.Studies have shown that autophagy plays an important role in the formation and development of cognitive impairment,and the phosphoinositide 3-kinase(PI3K)/Akt signaling pathway plays an important role in autophagy regulation.To investigate the role played by the PI3K/Akt signaling pathway in the electroacupuncture treatment of cerebral ischemia/reperfusion rat models,we first established a rat model of cerebral ischemia/reperfusion through the occlusion of the middle cerebral artery using the suture method.Starting at 2 hours after modeling,electroacupuncture was delivered at the Shenting(GV24)and Baihui(GV20)acupoints,with a dilatational wave(1-20 Hz frequency,2 mA intensity,6 V peak voltage),for 30 minutes/day over 8 consecutive days.Our results showed that electroacupuncture reduced the infarct volume in a rat model of cerebral ischemia/reperfusion injury,increased the mRNA expression levels of the PI3K/Akt signaling pathwayrelated factors Beclin-1,mammalian target of rapamycin(mTOR),and PI3K,increased the protein expression levels of phosphorylated Akt,Beclin-1,PI3K,and mTOR in the ischemic cerebral cortex,and simultaneously reduced p53 mRNA and protein expression levels.In the Morris water maze test,the latency to find the hidden platform was significantly shortened among rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.In the spatial probe test,the number of times that a rat crossed the target quadrant was increased in rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.Electroacupuncture stimulation applied to the Shenting(GV24)and Baihui(GV20)acupoints activated the PI3K/Akt signaling pathway and improved rat learning and memory impairment.This study was approved by the Animal Ethics Committee of the First Affiliated Hospital of Henan Unive
