Traditional Chinese medicine （TCM） has developed over thousands of years and has accumulated abundant clinical experience, forming a comprehensive and unique medical system. Emerging evidence has begun to illustrate...Traditional Chinese medicine （TCM） has developed over thousands of years and has accumulated abundant clinical experience, forming a comprehensive and unique medical system. Emerging evidence has begun to illustrate TCM as an area of important medical rediscoveries. This review article briefly introduced the concept, significance, and technology of network pharmacology based on network biology and systems biology. It focused on the theoretical system and potential prospect of TCM network applied in TCM research and development including predicting new drug targets, action mechanism, new drug discovery; evaluating pharmacodynamics, pharmacokinetics, safety, toxicology, quality control, and bioinformatics of drugs. We also discussed the opportunities and challenges in the development and application of network pharmacology in the modernization of TCM research.展开更多
栀子具有传统的益智开窍功效,但是其作用机制不明确。该研究以栀子传统益智开窍功效与抗阿尔茨海默症之间的关系为切入点,采用网络药理学方法研究预测栀子中主要入血成分的抗阿尔茨海默症药理作用机制,建立其"入血成分-靶标-通路-...栀子具有传统的益智开窍功效,但是其作用机制不明确。该研究以栀子传统益智开窍功效与抗阿尔茨海默症之间的关系为切入点,采用网络药理学方法研究预测栀子中主要入血成分的抗阿尔茨海默症药理作用机制,建立其"入血成分-靶标-通路-疾病"的网络药理学研究模式。研究采用活性成分筛选、靶点预测技术,确定栀子抗阿尔茨海默症的有效成分及作用靶点,利用生物信息注释数据库(DAVID)富集通路及生物过程,分子对接验证网络分析结果,阐述栀子抗阿尔茨海默症的作用机制。结果显示,栀子35个入血成分可能调控胆碱酯酶(cholinesterase,BCHE)和碳酸酐酶2(carbonic anhydrase 2,CA2)等48个靶点,显著影响转录因子活性、核受体活性、类固醇激素受体活性、酰胺结合和肽结合等11条生物过程及丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路、阿尔茨海默病、促性腺激素释放激素信号通路和雌激素信号途径等7条代谢通路。且分子对接验证显示,60%以上的有效成分与关键靶点具有结合活性,相关文献也显示结果所得活性成分能够抑制关键靶点MAPK1表达,说明结果可靠性较高。研究结果表明栀子可能通过"多成分、多靶点、多通路"发挥抗阿尔茨海默症的作用,为栀子的进一步研究及其产品开发提供了科学依据。展开更多
基金National Natural Science Foundation of China(81430096)China Drug Innovation Plan(2014ZX09507005,2014ZX09507005-003,2012ZX09304002,2012ZX09505001)
文摘Traditional Chinese medicine （TCM） has developed over thousands of years and has accumulated abundant clinical experience, forming a comprehensive and unique medical system. Emerging evidence has begun to illustrate TCM as an area of important medical rediscoveries. This review article briefly introduced the concept, significance, and technology of network pharmacology based on network biology and systems biology. It focused on the theoretical system and potential prospect of TCM network applied in TCM research and development including predicting new drug targets, action mechanism, new drug discovery; evaluating pharmacodynamics, pharmacokinetics, safety, toxicology, quality control, and bioinformatics of drugs. We also discussed the opportunities and challenges in the development and application of network pharmacology in the modernization of TCM research.
文摘栀子具有传统的益智开窍功效,但是其作用机制不明确。该研究以栀子传统益智开窍功效与抗阿尔茨海默症之间的关系为切入点,采用网络药理学方法研究预测栀子中主要入血成分的抗阿尔茨海默症药理作用机制,建立其"入血成分-靶标-通路-疾病"的网络药理学研究模式。研究采用活性成分筛选、靶点预测技术,确定栀子抗阿尔茨海默症的有效成分及作用靶点,利用生物信息注释数据库(DAVID)富集通路及生物过程,分子对接验证网络分析结果,阐述栀子抗阿尔茨海默症的作用机制。结果显示,栀子35个入血成分可能调控胆碱酯酶(cholinesterase,BCHE)和碳酸酐酶2(carbonic anhydrase 2,CA2)等48个靶点,显著影响转录因子活性、核受体活性、类固醇激素受体活性、酰胺结合和肽结合等11条生物过程及丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路、阿尔茨海默病、促性腺激素释放激素信号通路和雌激素信号途径等7条代谢通路。且分子对接验证显示,60%以上的有效成分与关键靶点具有结合活性,相关文献也显示结果所得活性成分能够抑制关键靶点MAPK1表达,说明结果可靠性较高。研究结果表明栀子可能通过"多成分、多靶点、多通路"发挥抗阿尔茨海默症的作用,为栀子的进一步研究及其产品开发提供了科学依据。
