建立调味粉中罂粟碱LAMP(loop-mediated isothermal amplification of DNA,LAMP)检测方法,并研制出配套试剂盒。根据罂粟碱的特异性基因序列设计引物,对12种罂粟、11种香辛料和1种虞美人核酸样品进行特异性、最佳温度筛选及敏感性实验,...建立调味粉中罂粟碱LAMP(loop-mediated isothermal amplification of DNA,LAMP)检测方法,并研制出配套试剂盒。根据罂粟碱的特异性基因序列设计引物,对12种罂粟、11种香辛料和1种虞美人核酸样品进行特异性、最佳温度筛选及敏感性实验,根据优化后的反应条件,组装试剂盒,并采用试剂盒对市售的8种调味粉进行检测。结果表明:12种罂粟和虞美人发生了特异性反应,最佳反应温度为65℃,LAMP检测最低浓度达70pg/μL,敏感性是PCR检测的10倍。市售8种调味粉中,有1种含有罂粟碱成分。实验所建立的检测方法及研制的试剂盒对调味粉中掺加罂粟粉检测具有重要意义。展开更多
Following the need for the use of environmentally friendly, renewable resource in industrial processes, this work explores the potential of an effective application in pilot scale of Afzelia bella seed as a coag-flocc...Following the need for the use of environmentally friendly, renewable resource in industrial processes, this work explores the potential of an effective application in pilot scale of Afzelia bella seed as a coag-flocculant. The study evaluates the coag-flocculation efficiency and functional kinetic parameter response to varying pH and dosage of coal washery effluent and ABC respectively. The maximum coag-flocculation performance is recorded at rate constant, K of 3.3333 x 10-3m3/kg.s, dosage of (0.3 and 0.2kg/m3);pH of 2 and coagulation period, τ1/2of 28.1216 s while the minimum is recorded at K of 1.6667 x 10-4m3/kg.s, dosage of 0.2kg/m3, pH of 10 and τ1/2 of 562.365 s. The least value of coag-flocculation efficiency, E (%)>89.00. Simulated and unsimulated values of rate constants Ks and K respectively are in close agreement, validating the concept of perikinetics. The potential of ABC as an effective organic coag-flocculant has been established. The results confirm that theory of rapid coagflocculation holds for the aggregation of coal washery effluent using ABC and at the conditions of the experiment.展开更多
文摘建立调味粉中罂粟碱LAMP(loop-mediated isothermal amplification of DNA,LAMP)检测方法,并研制出配套试剂盒。根据罂粟碱的特异性基因序列设计引物,对12种罂粟、11种香辛料和1种虞美人核酸样品进行特异性、最佳温度筛选及敏感性实验,根据优化后的反应条件,组装试剂盒,并采用试剂盒对市售的8种调味粉进行检测。结果表明:12种罂粟和虞美人发生了特异性反应,最佳反应温度为65℃,LAMP检测最低浓度达70pg/μL,敏感性是PCR检测的10倍。市售8种调味粉中,有1种含有罂粟碱成分。实验所建立的检测方法及研制的试剂盒对调味粉中掺加罂粟粉检测具有重要意义。
文摘Following the need for the use of environmentally friendly, renewable resource in industrial processes, this work explores the potential of an effective application in pilot scale of Afzelia bella seed as a coag-flocculant. The study evaluates the coag-flocculation efficiency and functional kinetic parameter response to varying pH and dosage of coal washery effluent and ABC respectively. The maximum coag-flocculation performance is recorded at rate constant, K of 3.3333 x 10-3m3/kg.s, dosage of (0.3 and 0.2kg/m3);pH of 2 and coagulation period, τ1/2of 28.1216 s while the minimum is recorded at K of 1.6667 x 10-4m3/kg.s, dosage of 0.2kg/m3, pH of 10 and τ1/2 of 562.365 s. The least value of coag-flocculation efficiency, E (%)>89.00. Simulated and unsimulated values of rate constants Ks and K respectively are in close agreement, validating the concept of perikinetics. The potential of ABC as an effective organic coag-flocculant has been established. The results confirm that theory of rapid coagflocculation holds for the aggregation of coal washery effluent using ABC and at the conditions of the experiment.
