摘要
目的对同一临床实验室采用透射比浊和散射比浊法测定血清免疫球蛋白IgA、IgG和IgM结果偏差进行评估。方法按照美国国家实验室标准委员会(NCCLS)EP9A文件,以散射比浊为比较方法,透射比浊为实验方法进行对比及偏差评估,将测定数据进行相关分析,并对两分析系统之间的预期偏差进行评估。结果IgA、IgG两法各浓度值测定结果的预期偏差均可以接受;IgM浓度为1.0g/L时,两法测定结果的预期偏差不能接受,其余浓度值测定结果的预期偏差可以接受。结论在使用性能较好的全自动生化分析仪及配套试剂的前提下,透射比浊法测定血清IgA、IgG和IgM的结果与散射比浊法的测定结果基本一致。
Objective To assess the outcome bias between turbidimetry and nephelometry in immunoassay for serum immunoglobulins.Methods According to the profile NCCLS-EP9-A,two different methods,nephelometry (as test method) and turbidimetry (as comparative method),were used for immunoassay of IgA,IgG and IgM.The correlation of obtained data was analyzed and the outcome bias between the two methods was assessed.Results The outcome bias between the two methods for IgA and IgG at various concentrations was acceptable.The outcome bias for IgM was also acceptable at all the concentrations except for 1.0 g/L.Conclusion The bias between turbidimetry and nephelometry for immunoassay of serum IgA, IgG and IgM was acceptable providing the automatic biochemical analyzer and the matching reagent are used.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2005年第3期189-191,共3页
Chinese Journal of Clinical Laboratory Science
