We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA It is based on loop-mediated isothermal amplification（LAMP）. Plain gold nanoparticles（AuNPs） are used to in...We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA It is based on loop-mediated isothermal amplification（LAMP）. Plain gold nanoparticles（AuNPs） are used to indicate the occurrence of LAME The amplified product is mixed with AuNPs in an optimized ratio, at which the deoxyribonucleotides（dNTPs） bind to the AuNPs via ligand-metal interactions and thus enhance AuNPs stability. If a target DNA is amplified, the dramatic reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for the identification of isothermal products, this method is simple and can be readily applied on site where instrumentation is inadequate or even lacking.展开更多
We have developed a microfluidic chip for colorimetric CH2＋ detection. In this chip, it is facile to do colorimet- ric Cu2＋ detection based on gold nanoparticles. This method has a dynamic detection range from 0.75 ...We have developed a microfluidic chip for colorimetric CH2＋ detection. In this chip, it is facile to do colorimet- ric Cu2＋ detection based on gold nanoparticles. This method has a dynamic detection range from 0.75 to 50 lamol/L with only 20μL solution including detection reagents and sample. The result can be readout by naked eye and pho-tographed by digital cameras. With the help of image processing software, we could measure the RGB value and calculate the Blue/Red ratio for more accurate quantification. Tap water could be detected in this portable chip.展开更多
A novel colorimetric chemosensor PBIOSi has been designed and synthesized. The addition of F to a THF solution of PBIOSi resulted in an obvious color change （from red to green） in a short time because of the specifi...A novel colorimetric chemosensor PBIOSi has been designed and synthesized. The addition of F to a THF solution of PBIOSi resulted in an obvious color change （from red to green） in a short time because of the specific cleavage of Si-O bond in PBIOSi by F-. It was interesting that PBIOSi showed excellent selectivity for F detection over other halides. The UV-Vis absorption and fluorescence were changed linearly with the concentration ofF over the range of 0- 10 μmol/L.展开更多
基金Supported by the National Natural Science Foundation of China（Nos.31070772, 31270907）, the Doctoral Program of Higher Education of China（No.20090101110136）, the Science and Technology Programs of Zhej iang Province of China（No.2011C 37029） and the Fund of State Key Laboratory of Industrial Control Technology of China.
文摘We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA It is based on loop-mediated isothermal amplification（LAMP）. Plain gold nanoparticles（AuNPs） are used to indicate the occurrence of LAME The amplified product is mixed with AuNPs in an optimized ratio, at which the deoxyribonucleotides（dNTPs） bind to the AuNPs via ligand-metal interactions and thus enhance AuNPs stability. If a target DNA is amplified, the dramatic reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for the identification of isothermal products, this method is simple and can be readily applied on site where instrumentation is inadequate or even lacking.
文摘We have developed a microfluidic chip for colorimetric CH2＋ detection. In this chip, it is facile to do colorimet- ric Cu2＋ detection based on gold nanoparticles. This method has a dynamic detection range from 0.75 to 50 lamol/L with only 20μL solution including detection reagents and sample. The result can be readout by naked eye and pho-tographed by digital cameras. With the help of image processing software, we could measure the RGB value and calculate the Blue/Red ratio for more accurate quantification. Tap water could be detected in this portable chip.
基金supported by the National Natural Science Foundation of China （Nos. 21272069, 20672035）, the Fundamental Research Funds for the Central Universities and Baihehua Group.
文摘A novel colorimetric chemosensor PBIOSi has been designed and synthesized. The addition of F to a THF solution of PBIOSi resulted in an obvious color change （from red to green） in a short time because of the specific cleavage of Si-O bond in PBIOSi by F-. It was interesting that PBIOSi showed excellent selectivity for F detection over other halides. The UV-Vis absorption and fluorescence were changed linearly with the concentration ofF over the range of 0- 10 μmol/L.
