AIM： To investigate the effect of delayed ethyl pyruvate （EP） delivery on distant organ injury, survival time and serum high mobility group box 1 （HMGB1） levels in rats with experimental severe acute pancreatitis...AIM： To investigate the effect of delayed ethyl pyruvate （EP） delivery on distant organ injury, survival time and serum high mobility group box 1 （HMGB1） levels in rats with experimental severe acute pancreatitis （SAP）. METHODS： A SAP model was induced by retrograde injection of artificial bile into the pancreatic ducts of rats. Animals were divided randomly into three groups （n = 32 in each group）： sham group, SAP group and delayed EP treatment group. The rats in the delayed EP treatment group received EP （30 mg/kg） at 12 h, 18 h and 30 h after induction of SAP. Animals were sacrificed, and samples were obtained at 24 h and 48 h after induction of SAP. Serum HMGB1, aspartate arninotransferase （AST）, alanine arninotransferase （ALT）, blood urea nitrogen （BUN）, and creatinine （Cr） levels were measured. Lung wet-to-dry-weight （W/D） ratios and histological scores were calculated to evaluate lung injury. Additional experiments were performed between SAP and delayed EP treatment groups to study the influence of EP on survival times of SAP rats. RESULTS： Delayed EP treatment significantly reduced serum HMGB1 levels, and protected against liver, renal and lung injury with reduced lung W/D ratios （8.22 ±0.42 vs 9.76 ± 0.45, P 〈 0.01）, pulmonary histological scores （7.1 ± 0.7 vs 8.4 ± 1.1, P 〈 0.01）, serum AST （667 ± 103 vs 1 368 ± 271, P 〈 0.01）, ALT （446 ± 91 vs 653 ± 98, P 〈 0.01） and Cr （1.2 ± 0.3 vs 1.8 ± 0.3, P 〈 0.01） levels. SAP rats had a median survival time of 44 h. Delayed EP treatment significantly prolonged median survival time to 72 h （P 〈 0.01）. CONCLUSION： Delayed EP therapy protects against distant organ injury and prolongs survival time via reduced serum HMGBllevels in rats with experimental SAP. EP may potentially serve as an effective new therapeutic option against the inflammatory response and multiple organ dysfunction syndrome （MODS） in SAP patients.展开更多
A 3-D time domain method is developed to investigate the gap influence on the wave threes for 3-D multiple floating structures. Special hydrodynamic resonance due to small gaps between multiple floating structures on ...A 3-D time domain method is developed to investigate the gap influence on the wave threes for 3-D multiple floating structures. Special hydrodynamic resonance due to small gaps between multiple floating structures on wave forces is examined. Strong and complicate hydrodynamic interactions between the floating bodies are observed and the numerical computations have proved the existence of the sharp peak force response on each floating body at some special resonant wave numbers. By comparison with the results from the frequency domain technique, the results obtained from the time domain method reveal the similar resonant phenomena and hydrodynamic interaction. The resonant wave numbers are also proved around kL-nπ （n = 1,2 ……∞） with a corresponding frequency shift. The strong hydrodynamic interaction feature is practically significant for the design of module structures and the links （connection） in whole the floating body system.展开更多
A distributed coordination algorithm is proposed to enhance the engagement of the multi-missile network in consideration of obstacle avoidance. To achieve a cooperative interception, the guidance law is developed in a...A distributed coordination algorithm is proposed to enhance the engagement of the multi-missile network in consideration of obstacle avoidance. To achieve a cooperative interception, the guidance law is developed in a simple form that consists of three individual components for tar- get capture, time coordination and obstacle avoidance. The distributed coordination algorithm enables a group of interceptor missiles to reach the target simultaneously, even if some member in the multi-missile network can only collect the information from nearest neighbors. The simula- tion results show that the guidance strategy provides a feasible tool to implement obstacle avoid- ance for the multi-missile network with satisfactory accuracy of target capture. The effects of the gain parameters are also discussed to evaluate the proposed approach.展开更多
The paper presents a new three-dimensional (3D) cooperative guidance approach by the receding horizon control (RHC) technique. The objective is to coordinate the impact time of a group of interceptor missiles against ...The paper presents a new three-dimensional (3D) cooperative guidance approach by the receding horizon control (RHC) technique. The objective is to coordinate the impact time of a group of interceptor missiles against the stationary target. The framework of a distributed RHC scheme is developed, in which each interceptor missile is assigned its own finite-horizon optimal control problem (FHOCP) and only shares the information with its neighbors. The solution of the local FHOCP is obtained by the constrained particle swarm optimization (PSO) method that is integrated into the distributed RHC framework with enhanced equality and inequality constraints. The numerical simulations show that the proposed guidance approach is feasible to implement the cooperative engagement with satisfied accuracy of target capture. Finally, the computation efficiency of the distributed RHC scheme is discussed in consideration of the PSO parameters, control update period and prediction horizon. (C) 2016 Chinese Society of Aeronautics and Astronautics. Production and hosting by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license.展开更多
AIM： To investigate the expression of multiple genes in Chinese jianpi herbal recipe Wei Chang An （WCA） in human gastric cancer cell line SGC-7901. METHODS： A human gastric adenocarcinoma cell line SGC-7902 grafte...AIM： To investigate the expression of multiple genes in Chinese jianpi herbal recipe Wei Chang An （WCA） in human gastric cancer cell line SGC-7901. METHODS： A human gastric adenocarcinoma cell line SGC-7902 grafted onto nude mice was used as the animal model. The mice were randomly divided into 3 groups, one control and the two representing experimental conditions. Animals in the two experimental groups received either WCA over a 34-d period or 5-fluorouracil （5-FU） over 6-d period starting at 8th d after grafting. Control animals received saline on an identical schedule. Animals were killed 41 d after being grafted. The expression profiles in paired WCA treated gastric cancer samples and the N.S. control samples were studied by using a cDNA array representing 14181 cDNA clusters. The alterations in gene expression levels were confirmed by Real-time Quantitative polymerase chain reaction （qPCR）. RESULTS： When compared with controls, the average tumor inhibitory rate in WCA group was 44.32% ± 5.67% and 5-FU 47.04% ± 22.33% （P 〈 0.01, respectively）. The average labeling index （LI） for PCNA in WCA group and 5-FU group was significantly decreased compared with the control group. Apoptotic index （AI） was significantly increased to 9.72% ± 4.52% using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate fluorescence nick end labeling （TUNEL） method in WCA group compared with the controls 2.45% ± 2.37%. 5-FU group was also found to have a significantly increased AI compared with the controls. The expression of cleaved Caspase-3 in WCA group and 5-FU group was significantly increased compared with the control group respectively. There were 45 different expressed sequence tags （ESTs） among the control sample pool and WCA sample pool. There were 24 ESTs up-regulated in WCA samples and 21 ESTs down-regulated. By using qPCR, the expression level of Stat3, rap2 interacting protein x （RIPX）, regulator of differentiation 1 （ROD1） and Bcl-2 was lower in 展开更多
基金The National Natural Science Foundation of China, No. 30600593
文摘AIM： To investigate the effect of delayed ethyl pyruvate （EP） delivery on distant organ injury, survival time and serum high mobility group box 1 （HMGB1） levels in rats with experimental severe acute pancreatitis （SAP）. METHODS： A SAP model was induced by retrograde injection of artificial bile into the pancreatic ducts of rats. Animals were divided randomly into three groups （n = 32 in each group）： sham group, SAP group and delayed EP treatment group. The rats in the delayed EP treatment group received EP （30 mg/kg） at 12 h, 18 h and 30 h after induction of SAP. Animals were sacrificed, and samples were obtained at 24 h and 48 h after induction of SAP. Serum HMGB1, aspartate arninotransferase （AST）, alanine arninotransferase （ALT）, blood urea nitrogen （BUN）, and creatinine （Cr） levels were measured. Lung wet-to-dry-weight （W/D） ratios and histological scores were calculated to evaluate lung injury. Additional experiments were performed between SAP and delayed EP treatment groups to study the influence of EP on survival times of SAP rats. RESULTS： Delayed EP treatment significantly reduced serum HMGB1 levels, and protected against liver, renal and lung injury with reduced lung W/D ratios （8.22 ±0.42 vs 9.76 ± 0.45, P 〈 0.01）, pulmonary histological scores （7.1 ± 0.7 vs 8.4 ± 1.1, P 〈 0.01）, serum AST （667 ± 103 vs 1 368 ± 271, P 〈 0.01）, ALT （446 ± 91 vs 653 ± 98, P 〈 0.01） and Cr （1.2 ± 0.3 vs 1.8 ± 0.3, P 〈 0.01） levels. SAP rats had a median survival time of 44 h. Delayed EP treatment significantly prolonged median survival time to 72 h （P 〈 0.01）. CONCLUSION： Delayed EP therapy protects against distant organ injury and prolongs survival time via reduced serum HMGBllevels in rats with experimental SAP. EP may potentially serve as an effective new therapeutic option against the inflammatory response and multiple organ dysfunction syndrome （MODS） in SAP patients.
基金the National Natural Science Foundation of China (Grant No. 50639020)the National High Technology Research and Development Program of China (863 Program, Grant No. 2006AA09Z332).
文摘A 3-D time domain method is developed to investigate the gap influence on the wave threes for 3-D multiple floating structures. Special hydrodynamic resonance due to small gaps between multiple floating structures on wave forces is examined. Strong and complicate hydrodynamic interactions between the floating bodies are observed and the numerical computations have proved the existence of the sharp peak force response on each floating body at some special resonant wave numbers. By comparison with the results from the frequency domain technique, the results obtained from the time domain method reveal the similar resonant phenomena and hydrodynamic interaction. The resonant wave numbers are also proved around kL-nπ （n = 1,2 ……∞） with a corresponding frequency shift. The strong hydrodynamic interaction feature is practically significant for the design of module structures and the links （connection） in whole the floating body system.
基金co-supported by the National Natural Science Foundation of China(Nos.61273349 and 61175109)the Aeronautical Science Foundation of China(Nos.2014ZA18004 and 2013ZA18001)
文摘A distributed coordination algorithm is proposed to enhance the engagement of the multi-missile network in consideration of obstacle avoidance. To achieve a cooperative interception, the guidance law is developed in a simple form that consists of three individual components for tar- get capture, time coordination and obstacle avoidance. The distributed coordination algorithm enables a group of interceptor missiles to reach the target simultaneously, even if some member in the multi-missile network can only collect the information from nearest neighbors. The simula- tion results show that the guidance strategy provides a feasible tool to implement obstacle avoid- ance for the multi-missile network with satisfactory accuracy of target capture. The effects of the gain parameters are also discussed to evaluate the proposed approach.
基金co-supported by the National Natural Science Foundation of China(Nos. 61273349 and 61573043)
文摘The paper presents a new three-dimensional (3D) cooperative guidance approach by the receding horizon control (RHC) technique. The objective is to coordinate the impact time of a group of interceptor missiles against the stationary target. The framework of a distributed RHC scheme is developed, in which each interceptor missile is assigned its own finite-horizon optimal control problem (FHOCP) and only shares the information with its neighbors. The solution of the local FHOCP is obtained by the constrained particle swarm optimization (PSO) method that is integrated into the distributed RHC framework with enhanced equality and inequality constraints. The numerical simulations show that the proposed guidance approach is feasible to implement the cooperative engagement with satisfied accuracy of target capture. Finally, the computation efficiency of the distributed RHC scheme is discussed in consideration of the PSO parameters, control update period and prediction horizon. (C) 2016 Chinese Society of Aeronautics and Astronautics. Production and hosting by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license.
基金E-institutes of Shanghai Municipal Education Commission, No. E03008The Major State Basic Research Development Program of China (973 Program), No. 2006CB 504604Shanghai Leading Academic Discipline Project, No. Y0302
文摘AIM： To investigate the expression of multiple genes in Chinese jianpi herbal recipe Wei Chang An （WCA） in human gastric cancer cell line SGC-7901. METHODS： A human gastric adenocarcinoma cell line SGC-7902 grafted onto nude mice was used as the animal model. The mice were randomly divided into 3 groups, one control and the two representing experimental conditions. Animals in the two experimental groups received either WCA over a 34-d period or 5-fluorouracil （5-FU） over 6-d period starting at 8th d after grafting. Control animals received saline on an identical schedule. Animals were killed 41 d after being grafted. The expression profiles in paired WCA treated gastric cancer samples and the N.S. control samples were studied by using a cDNA array representing 14181 cDNA clusters. The alterations in gene expression levels were confirmed by Real-time Quantitative polymerase chain reaction （qPCR）. RESULTS： When compared with controls, the average tumor inhibitory rate in WCA group was 44.32% ± 5.67% and 5-FU 47.04% ± 22.33% （P 〈 0.01, respectively）. The average labeling index （LI） for PCNA in WCA group and 5-FU group was significantly decreased compared with the control group. Apoptotic index （AI） was significantly increased to 9.72% ± 4.52% using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate fluorescence nick end labeling （TUNEL） method in WCA group compared with the controls 2.45% ± 2.37%. 5-FU group was also found to have a significantly increased AI compared with the controls. The expression of cleaved Caspase-3 in WCA group and 5-FU group was significantly increased compared with the control group respectively. There were 45 different expressed sequence tags （ESTs） among the control sample pool and WCA sample pool. There were 24 ESTs up-regulated in WCA samples and 21 ESTs down-regulated. By using qPCR, the expression level of Stat3, rap2 interacting protein x （RIPX）, regulator of differentiation 1 （ROD1） and Bcl-2 was lower in
