Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase system.Its incomplete oxidation system has many...Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase system.Its incomplete oxidation system has many crucial industrial applications.However,it also leads to slow growth and low biomass,requiring further metabolic modification for balancing the cell growth and incomplete oxidation process.As a non-model strain,G.oxydans lacks efficient genome editing tools and cannot perform rapid multi-gene editing and complex metabolic network regulation.In the last 15 years,our laboratory attempted to deploy multiple CRISPR/Cas systems in different G.oxydans strains and found none of them as functional.In this study,Cpf1-based or dCpf1-based CRISPRi was constructed to explore the targeted binding ability of Cpf1,while Cpf1–FokI was deployed to study its nuclease activity.A study on Cpf1 found that the CRISPR/Cpf1 system could locate the target genes in G.oxydans but lacked the nuclease cleavage activity.Therefore,the CRISPR/Cpf1–FokI system based on FokI nuclease was constructed.Single-gene knockout with efficiency up to 100%and double-gene iterative editing were achieved in G.oxydans.Using this system,AcrVA6,the anti-CRISPR protein of G.oxydans was discovered for the first time,and efficient genome editing was realized.展开更多
目的研究危害分析的临界控制点(hazard analysis critical control point,HACCP)在多维类片剂保健品生产中的应用,更好地保障生产过程中质量安全。方法利用HACCP原理,对多维类片剂保健品生产过程进行风险分析,制定危害评价程序,确定关...目的研究危害分析的临界控制点(hazard analysis critical control point,HACCP)在多维类片剂保健品生产中的应用,更好地保障生产过程中质量安全。方法利用HACCP原理,对多维类片剂保健品生产过程进行风险分析,制定危害评价程序,确定关键控制点,从人、机、料、法、环、测、软、辅、公等9个方面进行分析,并制定相应控制措施。结果将HACCP体系运用到多维类片剂保健品生产各个环节中,制定详细有效的控制计划,利用所制定控制计划控制多维类片剂保健品生产过程质量风险。结论采用HACCP体系对多维类片剂保健品生产过程进行控制,更好地保障了保健品安全,降低企业管理成本,提升经济收益。展开更多
目的探讨复合营养维生素补充治疗对3～6岁儿童营养、发育状况的影响。方法选取2016年1月至2017年1月来安康市中心医院儿童保健科体检的320名健康儿童为研究对象,按照随机数表法将入选儿童分为观察组和照组,每组160名。对照组儿童单纯给...目的探讨复合营养维生素补充治疗对3～6岁儿童营养、发育状况的影响。方法选取2016年1月至2017年1月来安康市中心医院儿童保健科体检的320名健康儿童为研究对象,按照随机数表法将入选儿童分为观察组和照组,每组160名。对照组儿童单纯给予补充维生素A,观察组儿童给予补充复合营养维生素,均连续服用6个月。分别于干预前后测量两组儿童的身高、体质量、血清维生素A、钙、铁、锌水平,比较两组儿童营养不良发生情况。结果干预前观察组和对照组儿童的身高[(103.4±5.7) cm vs (103.2±5.3) cm]、体质量[(16.8±1.5) kg vs(16.5±1.4) kg]、维生素A [(120.5±7.8)μmol/L vs (120.3±7.7)μmol/L]、钙[(2.52±0.23) mmol/L vs (2.50±0.21) mmol/L]、铁[(17.5±5.0)μmol/L vs (17.4±5.1)μmol/L]及锌[(12.3±2.1)μmol/L vs (12.2±2.0)μmol/L]比较差异均无统计学意义(P<0.05);干预后,两组儿童的身高、体质量、维生素A、钙、铁及锌水平均升高,且观察组改善情况优于对照组[身高:(109.6±6.2) cm vs (106.4±5.9) cm;体质量:(19.7±1.8) kg vs (17.4±1.6) kg;维生素A:(125.6±8.6)μmol/L vs (122.9±7.9)μmol/L;钙:(2.58±0.25) mmol/L vs (2.37±0.20) mmol/L;铁:(25.2±7.1)μmol/L vs (21.1±6.8)μmol/L;锌:(15.8±3.1)μmol/L vs(13.4±2.8)μmol/L],差异均有统计学意义(P<0.05);干预后,两组儿童的低体质量、消瘦、生长迟缓及总营养不良发生率较干预前略有降低,但差异均无统计学意义(P>0.05)。结论儿童营养不良的发生可能与微量营养素缺乏有关,服用复合微量营养素补充剂可以明显改善3～6岁儿童的营养状况,促进儿童的正常生长发育。展开更多
基金This work was supported by the National Natural Science Foundation of China(Key Program,31830068),China National Postdoctoral Program for Innovative Talents(BX20230147),China Postdoctoral Science Foundation(2023M741406),Jiangsu Funding Program for Excellent Postdoctoral Talent(2023ZB631).
文摘Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase system.Its incomplete oxidation system has many crucial industrial applications.However,it also leads to slow growth and low biomass,requiring further metabolic modification for balancing the cell growth and incomplete oxidation process.As a non-model strain,G.oxydans lacks efficient genome editing tools and cannot perform rapid multi-gene editing and complex metabolic network regulation.In the last 15 years,our laboratory attempted to deploy multiple CRISPR/Cas systems in different G.oxydans strains and found none of them as functional.In this study,Cpf1-based or dCpf1-based CRISPRi was constructed to explore the targeted binding ability of Cpf1,while Cpf1–FokI was deployed to study its nuclease activity.A study on Cpf1 found that the CRISPR/Cpf1 system could locate the target genes in G.oxydans but lacked the nuclease cleavage activity.Therefore,the CRISPR/Cpf1–FokI system based on FokI nuclease was constructed.Single-gene knockout with efficiency up to 100%and double-gene iterative editing were achieved in G.oxydans.Using this system,AcrVA6,the anti-CRISPR protein of G.oxydans was discovered for the first time,and efficient genome editing was realized.
文摘目的研究危害分析的临界控制点(hazard analysis critical control point,HACCP)在多维类片剂保健品生产中的应用,更好地保障生产过程中质量安全。方法利用HACCP原理,对多维类片剂保健品生产过程进行风险分析,制定危害评价程序,确定关键控制点,从人、机、料、法、环、测、软、辅、公等9个方面进行分析,并制定相应控制措施。结果将HACCP体系运用到多维类片剂保健品生产各个环节中,制定详细有效的控制计划,利用所制定控制计划控制多维类片剂保健品生产过程质量风险。结论采用HACCP体系对多维类片剂保健品生产过程进行控制,更好地保障了保健品安全,降低企业管理成本,提升经济收益。
文摘目的探讨复合营养维生素补充治疗对3～6岁儿童营养、发育状况的影响。方法选取2016年1月至2017年1月来安康市中心医院儿童保健科体检的320名健康儿童为研究对象,按照随机数表法将入选儿童分为观察组和照组,每组160名。对照组儿童单纯给予补充维生素A,观察组儿童给予补充复合营养维生素,均连续服用6个月。分别于干预前后测量两组儿童的身高、体质量、血清维生素A、钙、铁、锌水平,比较两组儿童营养不良发生情况。结果干预前观察组和对照组儿童的身高[(103.4±5.7) cm vs (103.2±5.3) cm]、体质量[(16.8±1.5) kg vs(16.5±1.4) kg]、维生素A [(120.5±7.8)μmol/L vs (120.3±7.7)μmol/L]、钙[(2.52±0.23) mmol/L vs (2.50±0.21) mmol/L]、铁[(17.5±5.0)μmol/L vs (17.4±5.1)μmol/L]及锌[(12.3±2.1)μmol/L vs (12.2±2.0)μmol/L]比较差异均无统计学意义(P<0.05);干预后,两组儿童的身高、体质量、维生素A、钙、铁及锌水平均升高,且观察组改善情况优于对照组[身高:(109.6±6.2) cm vs (106.4±5.9) cm;体质量:(19.7±1.8) kg vs (17.4±1.6) kg;维生素A:(125.6±8.6)μmol/L vs (122.9±7.9)μmol/L;钙:(2.58±0.25) mmol/L vs (2.37±0.20) mmol/L;铁:(25.2±7.1)μmol/L vs (21.1±6.8)μmol/L;锌:(15.8±3.1)μmol/L vs(13.4±2.8)μmol/L],差异均有统计学意义(P<0.05);干预后,两组儿童的低体质量、消瘦、生长迟缓及总营养不良发生率较干预前略有降低,但差异均无统计学意义(P>0.05)。结论儿童营养不良的发生可能与微量营养素缺乏有关,服用复合微量营养素补充剂可以明显改善3～6岁儿童的营养状况,促进儿童的正常生长发育。
