A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific...A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers.展开更多
A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing o...A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.展开更多
基金This work was supported by the National"863"High Technology Foundation(Grant Nos.2001AA211121 and 2002AA-211031)the National"948"Foundation(Grant No.201012).
文摘A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers.
文摘A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.
