Recent advances in hydrocarbon exploration have been made in the Member Deng-2 marginal microbial mound-bank complex reservoirs of the Dengying Formation in the western Sichuan Basin, SW China,where the depositional p...Recent advances in hydrocarbon exploration have been made in the Member Deng-2 marginal microbial mound-bank complex reservoirs of the Dengying Formation in the western Sichuan Basin, SW China,where the depositional process is regarded confusing. The microfacies, construction types, and depositional model of the Member Deng-2 marginal microbial mound-bank complex have been investigated using unmanned aerial vehicle photography, outcrop section investigation, thin section identification,and seismic reflections in the southwestern Sichuan Basin. The microbialite lithologic textures in this region include thrombolite, dendrolite, stromatolite, fenestral stromatolite, spongiostromata stone,oncolite, aggregated grainstone, and botryoidal grapestone. Based on the comprehensive analysis of“depositional fabrics-lithology-microfacies”, an association between a fore mound, mound framework,and back mound subfacies has been proposed based on water depth, current direction, energy level and lithologic assemblages. The microfacies of the mound base, mound core, mound flank, mound cap, and mound flat could be recognized among the mound framework subfacies. Two construction types of marginal microbial mound-bank complex have been determined based on deposition location, mound scale, migration direction, and sedimentary facies association. Type Jinkouhe microbial mound constructions(TJMMCs) develop along the windward margin owing to their proximity to the seaward subfacies fore mound, with a northeastwardly migrated microbial mound on top of the mud mound,exhibiting the characteristics of large-sized mounds and small-sized banks in the surrounding area. Type E'bian microbial mound constructions(TEMMCs) primarily occur on the leeward margin, resulting from the presence of onshore back mound subfacies, with the smaller southwestward migrated microbial mounds existing on a thicker microbial flat. The platform margin microbial mound depositional model can be correlated with certain lateral comparison profile and seismic reflectio展开更多
Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the bio...Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of microorganisms,it requires complicated biological procedures.Herein,we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism.Covalent linking of oligo(p-phenylenevinylene)(OPV)and cyclopentadienyl rhodium(Ⅲ)bipyridine offers intracellular metal catalyst(OPV-Rh).The OPV-Rh complex displayed certain resistance to toxic biomolecules,which guaranteed its catalytic activity in complicated biological systems.With uptake by Gramnegative bacterium Ralstonia eutropha H16(R.eutropha H16),the OPV-Rh complex promotes the transformation of intracellular NADP+to NADPH,which further enhances the biosynthesis of polyhydroxybutyrate(PHB)by this microorganism.This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly.展开更多
选取前期实验中从长庆油田措施废液集中处理后残渣中筛选的3株高效石油烃降解菌D1、D2、D5作为研究对象,采用吸附法制备固定化菌剂,制备过程的最佳条件为:秸秆和草炭固定化菌剂的最佳固定化时间均为36 h,木炭的最佳固定化时间为30 h;秸...选取前期实验中从长庆油田措施废液集中处理后残渣中筛选的3株高效石油烃降解菌D1、D2、D5作为研究对象,采用吸附法制备固定化菌剂,制备过程的最佳条件为:秸秆和草炭固定化菌剂的最佳固定化时间均为36 h,木炭的最佳固定化时间为30 h;秸秆固定化菌剂和草炭固定化菌剂的最优载体加入量为3.0g/100 m L菌悬液;木炭固定化菌剂的最优载体加入量为2.0 g/100 m L菌悬液。秸秆固定化的最佳pH为7.0,草炭和木炭固定化的最佳pH为7.5;三种载体固定化菌剂的最佳固定化温度均为35℃。在未灭菌的含油土壤40d的室内原位模拟修复过程中,各固定化菌剂对石油烃的降解率大小依次为:草炭固定化菌剂74.12%>木炭固定化菌剂70.25%>秸秆固定化菌剂60.74%>游离混合菌35.48%>不加菌对照11.98%。在灭菌的含油土壤的修复过程中,几种处理方式对石油烃的降解率大小依次为:木炭固定化菌剂70.75%>草炭固定化菌剂69.90%>秸秆固定化菌剂68.28%>游离混合菌44.30%>不加菌对照2.21%。展开更多
Marine sediment microbial fuel cell(MSMFCs)can be utilized as a long lasting power source to drive small instruments to work for long time on ocean floor and its higher power has a significant meaning for practical ap...Marine sediment microbial fuel cell(MSMFCs)can be utilized as a long lasting power source to drive small instruments to work for long time on ocean floor and its higher power has a significant meaning for practical application.Anode modification can greatly improve the performance of MSMFCs.Herein,humic acid(HA)and humic acid-iron ion complex(HA-Fe)were used to modify the anode for constructing a better MSMFCs.The results indicated that HA-Fe modified anode,better than HA modification,significantly improved the MSMFCs cell power output.The maximum power density of HA-Fe modified MSMFCs is 165.3 mW m−2,which are 6.5-folds of blank MSMFCs.The number of microorganisms on anode,redox activity,and relative kinetic activity were 1.8-,6.1-,and 13.1-folds of blank MSMFCs,respectively.The MSMFCs improvement would be attributed to the electron transfer media of HA and the valence conversion of Fe ions.A synergistic interaction between the naturally occurring HA and Fe ions on the anodic surface in marine sediments would make the modified anodes have‘renewable’characteristics,which is beneficial for the MSMFCs to maintain its long-term higher power.展开更多
基金jointly funded by projects supported by the National Natural Science Foundation of China(Grant No.41872150)the Joint Funds of the National Natural Science Foundation of China(Grant No.U19B6003)Major Scientific and Technological Projects of CNPC during the 13th five-year plan(No.2019A-02-10)。
文摘Recent advances in hydrocarbon exploration have been made in the Member Deng-2 marginal microbial mound-bank complex reservoirs of the Dengying Formation in the western Sichuan Basin, SW China,where the depositional process is regarded confusing. The microfacies, construction types, and depositional model of the Member Deng-2 marginal microbial mound-bank complex have been investigated using unmanned aerial vehicle photography, outcrop section investigation, thin section identification,and seismic reflections in the southwestern Sichuan Basin. The microbialite lithologic textures in this region include thrombolite, dendrolite, stromatolite, fenestral stromatolite, spongiostromata stone,oncolite, aggregated grainstone, and botryoidal grapestone. Based on the comprehensive analysis of“depositional fabrics-lithology-microfacies”, an association between a fore mound, mound framework,and back mound subfacies has been proposed based on water depth, current direction, energy level and lithologic assemblages. The microfacies of the mound base, mound core, mound flank, mound cap, and mound flat could be recognized among the mound framework subfacies. Two construction types of marginal microbial mound-bank complex have been determined based on deposition location, mound scale, migration direction, and sedimentary facies association. Type Jinkouhe microbial mound constructions(TJMMCs) develop along the windward margin owing to their proximity to the seaward subfacies fore mound, with a northeastwardly migrated microbial mound on top of the mud mound,exhibiting the characteristics of large-sized mounds and small-sized banks in the surrounding area. Type E'bian microbial mound constructions(TEMMCs) primarily occur on the leeward margin, resulting from the presence of onshore back mound subfacies, with the smaller southwestward migrated microbial mounds existing on a thicker microbial flat. The platform margin microbial mound depositional model can be correlated with certain lateral comparison profile and seismic reflectio
基金the National Natural Science Foundation of China(21533012,21661132006)。
文摘Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of microorganisms,it requires complicated biological procedures.Herein,we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism.Covalent linking of oligo(p-phenylenevinylene)(OPV)and cyclopentadienyl rhodium(Ⅲ)bipyridine offers intracellular metal catalyst(OPV-Rh).The OPV-Rh complex displayed certain resistance to toxic biomolecules,which guaranteed its catalytic activity in complicated biological systems.With uptake by Gramnegative bacterium Ralstonia eutropha H16(R.eutropha H16),the OPV-Rh complex promotes the transformation of intracellular NADP+to NADPH,which further enhances the biosynthesis of polyhydroxybutyrate(PHB)by this microorganism.This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly.
文摘选取前期实验中从长庆油田措施废液集中处理后残渣中筛选的3株高效石油烃降解菌D1、D2、D5作为研究对象,采用吸附法制备固定化菌剂,制备过程的最佳条件为:秸秆和草炭固定化菌剂的最佳固定化时间均为36 h,木炭的最佳固定化时间为30 h;秸秆固定化菌剂和草炭固定化菌剂的最优载体加入量为3.0g/100 m L菌悬液;木炭固定化菌剂的最优载体加入量为2.0 g/100 m L菌悬液。秸秆固定化的最佳pH为7.0,草炭和木炭固定化的最佳pH为7.5;三种载体固定化菌剂的最佳固定化温度均为35℃。在未灭菌的含油土壤40d的室内原位模拟修复过程中,各固定化菌剂对石油烃的降解率大小依次为:草炭固定化菌剂74.12%>木炭固定化菌剂70.25%>秸秆固定化菌剂60.74%>游离混合菌35.48%>不加菌对照11.98%。在灭菌的含油土壤的修复过程中,几种处理方式对石油烃的降解率大小依次为:木炭固定化菌剂70.75%>草炭固定化菌剂69.90%>秸秆固定化菌剂68.28%>游离混合菌44.30%>不加菌对照2.21%。
基金supported by the National Natural Science Foundation of China(No.22075262).
文摘Marine sediment microbial fuel cell(MSMFCs)can be utilized as a long lasting power source to drive small instruments to work for long time on ocean floor and its higher power has a significant meaning for practical application.Anode modification can greatly improve the performance of MSMFCs.Herein,humic acid(HA)and humic acid-iron ion complex(HA-Fe)were used to modify the anode for constructing a better MSMFCs.The results indicated that HA-Fe modified anode,better than HA modification,significantly improved the MSMFCs cell power output.The maximum power density of HA-Fe modified MSMFCs is 165.3 mW m−2,which are 6.5-folds of blank MSMFCs.The number of microorganisms on anode,redox activity,and relative kinetic activity were 1.8-,6.1-,and 13.1-folds of blank MSMFCs,respectively.The MSMFCs improvement would be attributed to the electron transfer media of HA and the valence conversion of Fe ions.A synergistic interaction between the naturally occurring HA and Fe ions on the anodic surface in marine sediments would make the modified anodes have‘renewable’characteristics,which is beneficial for the MSMFCs to maintain its long-term higher power.
