目的建立卡西酮、甲卡西酮、4-甲基甲卡西酮的LC-MS/MS定性定量分析方法。方法采用Agilent6460三重串联四极杆液质联用仪(LC/QQQ),样品用甲醇直接提取,采用Agilent Zorbax○R Eclipse Plus C18色谱柱(100mm×2.1mm,1.8μm),流动相为...目的建立卡西酮、甲卡西酮、4-甲基甲卡西酮的LC-MS/MS定性定量分析方法。方法采用Agilent6460三重串联四极杆液质联用仪(LC/QQQ),样品用甲醇直接提取,采用Agilent Zorbax○R Eclipse Plus C18色谱柱(100mm×2.1mm,1.8μm),流动相为0.1%甲酸和乙腈,梯度洗脱,流速为0.3mL/min,进样体积为3μL。质谱应用ESI源、正离子模式、多反应监测(MRM)方式检测卡西酮、甲卡西酮、4-甲基甲卡西酮。结果卡西酮的线性范围为1ng/mL^25000ng/mL,甲卡西酮的线性范围为0.1ng/mL^10000ng/mL,4-甲基甲卡西酮的线性范围为1ng/mL^10000ng/mL。结论该方法简单、准确,灵敏度高,可以满足案件鉴定工作的需要。展开更多
Methcathinone(MCAT)belongs to the designer drugs called synthetic cathinones,which are abused worldwide for recreational purposes.It has strong stimulant effects,including enhanced euphoria,sensation,alertness,and emp...Methcathinone(MCAT)belongs to the designer drugs called synthetic cathinones,which are abused worldwide for recreational purposes.It has strong stimulant effects,including enhanced euphoria,sensation,alertness,and empathy.However,little is known about how MCAT modulates neuronal activity in vivo.Here,we evaluated the effect of MCAT on neuronal activity with a series of functional approaches.C-Fos immunostaining showed that MCAT increased the number of activated neurons by 6-fold,especially in sensory and motor cortices,striatum,and midbrain motor nuclei.In vivo single-unit recording and two-photon Ca^(2+) imaging revealed that a large proportion of neurons increased spiking activity upon MCAT administration.Notably,MCAT induced a strong de-correlation of population activity and increased trial-to-trial reliability,specifically during a natural movie stimulus.It improved the information-processing efficiency by enhancing the single-neuron coding capacity,suggesting a cortical network mechanism of the enhanced perception produced by psychoactive stimulants.展开更多
文摘目的建立卡西酮、甲卡西酮、4-甲基甲卡西酮的LC-MS/MS定性定量分析方法。方法采用Agilent6460三重串联四极杆液质联用仪(LC/QQQ),样品用甲醇直接提取,采用Agilent Zorbax○R Eclipse Plus C18色谱柱(100mm×2.1mm,1.8μm),流动相为0.1%甲酸和乙腈,梯度洗脱,流速为0.3mL/min,进样体积为3μL。质谱应用ESI源、正离子模式、多反应监测(MRM)方式检测卡西酮、甲卡西酮、4-甲基甲卡西酮。结果卡西酮的线性范围为1ng/mL^25000ng/mL,甲卡西酮的线性范围为0.1ng/mL^10000ng/mL,4-甲基甲卡西酮的线性范围为1ng/mL^10000ng/mL。结论该方法简单、准确,灵敏度高,可以满足案件鉴定工作的需要。
基金supported by the National Natural Science Foundation of China(31871089 and 31871028)Junior Thousand Talents Program of China,Fundamental Research Funds for the Central Universities(HUST:2172019kfyXKJC077 and HUST:2172019kfyRCPY064)Key Laboratory of Forensic Toxicology,Ministry of Public Security of China(Beijing Municipal Public Security Bureau:2020FTDWFX02 and 2019FTDWFX06).
文摘Methcathinone(MCAT)belongs to the designer drugs called synthetic cathinones,which are abused worldwide for recreational purposes.It has strong stimulant effects,including enhanced euphoria,sensation,alertness,and empathy.However,little is known about how MCAT modulates neuronal activity in vivo.Here,we evaluated the effect of MCAT on neuronal activity with a series of functional approaches.C-Fos immunostaining showed that MCAT increased the number of activated neurons by 6-fold,especially in sensory and motor cortices,striatum,and midbrain motor nuclei.In vivo single-unit recording and two-photon Ca^(2+) imaging revealed that a large proportion of neurons increased spiking activity upon MCAT administration.Notably,MCAT induced a strong de-correlation of population activity and increased trial-to-trial reliability,specifically during a natural movie stimulus.It improved the information-processing efficiency by enhancing the single-neuron coding capacity,suggesting a cortical network mechanism of the enhanced perception produced by psychoactive stimulants.
