GIS中盘式绝缘子的电气性能是设计者关注的重点,盘式绝缘子在制造的过程中,由于浇注工艺的原因会在金属法兰处留有一椭圆切面的浇注孔,内部填充环氧树脂,在环氧树脂和金属法兰交界处会产生一定的电场畸变。主要研究金属法兰开孔尺寸大...GIS中盘式绝缘子的电气性能是设计者关注的重点,盘式绝缘子在制造的过程中,由于浇注工艺的原因会在金属法兰处留有一椭圆切面的浇注孔,内部填充环氧树脂,在环氧树脂和金属法兰交界处会产生一定的电场畸变。主要研究金属法兰开孔尺寸大小和结构对盆式绝缘子表面和浇注孔表面电场强度的影响。研究表明:大尺寸浇注孔表面的场强最大值为0.85 k V/mm,要高于小尺寸浇注孔表面电场强度最大值0.38 kV/mm。说明开孔越小,浇注孔表面电场强度最大值越小,在满足测量要求情况下,开孔越小越好。展开更多
Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal d...Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments.In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 （Schwann cell marker）, glial fibrillary acidic protein （astrocyte marker）, and brain-derived neurotrophic factor and nerve growth factor （neurotrophic factors） mRNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site （1 × 106 cells）. DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in th展开更多
文摘GIS中盘式绝缘子的电气性能是设计者关注的重点,盘式绝缘子在制造的过程中,由于浇注工艺的原因会在金属法兰处留有一椭圆切面的浇注孔,内部填充环氧树脂,在环氧树脂和金属法兰交界处会产生一定的电场畸变。主要研究金属法兰开孔尺寸大小和结构对盆式绝缘子表面和浇注孔表面电场强度的影响。研究表明:大尺寸浇注孔表面的场强最大值为0.85 k V/mm,要高于小尺寸浇注孔表面电场强度最大值0.38 kV/mm。说明开孔越小,浇注孔表面电场强度最大值越小,在满足测量要求情况下,开孔越小越好。
基金supported by a grant of the Korea Health Technology R & D Project through the Korea Health Industry Development Institute(KHIDI)funded by the Ministry of Health&Welfare,Republic of Korea(grant number:HI15C1535)
文摘Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments.In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 （Schwann cell marker）, glial fibrillary acidic protein （astrocyte marker）, and brain-derived neurotrophic factor and nerve growth factor （neurotrophic factors） mRNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site （1 × 106 cells）. DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in th
