With the content and recovery rate of flavonoids as indexes,a method for separation and purification of flavonoids from citrus with YWD-06C type resin is studied in this paper.At the same time the effect of purificati...With the content and recovery rate of flavonoids as indexes,a method for separation and purification of flavonoids from citrus with YWD-06C type resin is studied in this paper.At the same time the effect of purification and no purification citrus flavonoids on scavenging anthracene and hydroxyl free radical is studied.The results are as flollows: the content of flavonoids is 38.7% with the recovery rate of 49.8% after purification with YWD-06C type resin,the EC50 of purification and no purification citrus flavonoids on scavenging anthracene are 0.07g/L,0.80g/L respectivily,and on scavenging hydroxyl free radical are 0.61g/L,0.19g/L repectirily.The best conditions for separating and purificating citrus flavonoids is that:with the flavones content in the liquid is 1.22g/L,pH=3,the volume of water is about 15 times the dry resin quality is first used(pH=4.0),then 8 times ethyl acetate is used,and the 8 times ethanol(50%) is used in the last time.The YWD-06C type resin can be used 3 times repeatedly.展开更多
采用不同大孔吸附树脂分离纯化猪毛菜总黄酮,并对纯化后的总黄酮进行体外抗氧化活性测试。通过考察影响树脂静态和动态吸附与洗脱的主要因素,确定猪毛菜总黄酮分离纯化优化工艺条件。静态吸附实验表明,AB-8树脂分离纯化效果较好,并且吸...采用不同大孔吸附树脂分离纯化猪毛菜总黄酮,并对纯化后的总黄酮进行体外抗氧化活性测试。通过考察影响树脂静态和动态吸附与洗脱的主要因素,确定猪毛菜总黄酮分离纯化优化工艺条件。静态吸附实验表明,AB-8树脂分离纯化效果较好,并且吸附符合Langmuir和Freundlich方程。动态吸附和解吸的最佳工艺条件为：上样液质量浓度1.25 g/L、p H=4.5、上样流速2 m L/min、上样量2.5 BV（BV指树脂柱内装载树脂的体积）、洗脱剂为体积分数80%的乙醇溶液、洗脱流速1.0 m L/min,洗脱剂用量4 BV。所得洗脱液中黄酮质量分数从纯化前10.20%增加到纯化后51.89%,回收率为84.43%。体外实验表明,纯化后的黄酮可以清除羟自由基和超氧阴离子自由基,并有较好的还原力。纯化后的黄酮可以作为一种潜在的天然抗氧化剂。展开更多
文摘With the content and recovery rate of flavonoids as indexes,a method for separation and purification of flavonoids from citrus with YWD-06C type resin is studied in this paper.At the same time the effect of purification and no purification citrus flavonoids on scavenging anthracene and hydroxyl free radical is studied.The results are as flollows: the content of flavonoids is 38.7% with the recovery rate of 49.8% after purification with YWD-06C type resin,the EC50 of purification and no purification citrus flavonoids on scavenging anthracene are 0.07g/L,0.80g/L respectivily,and on scavenging hydroxyl free radical are 0.61g/L,0.19g/L repectirily.The best conditions for separating and purificating citrus flavonoids is that:with the flavones content in the liquid is 1.22g/L,pH=3,the volume of water is about 15 times the dry resin quality is first used(pH=4.0),then 8 times ethyl acetate is used,and the 8 times ethanol(50%) is used in the last time.The YWD-06C type resin can be used 3 times repeatedly.
文摘采用不同大孔吸附树脂分离纯化猪毛菜总黄酮,并对纯化后的总黄酮进行体外抗氧化活性测试。通过考察影响树脂静态和动态吸附与洗脱的主要因素,确定猪毛菜总黄酮分离纯化优化工艺条件。静态吸附实验表明,AB-8树脂分离纯化效果较好,并且吸附符合Langmuir和Freundlich方程。动态吸附和解吸的最佳工艺条件为：上样液质量浓度1.25 g/L、p H=4.5、上样流速2 m L/min、上样量2.5 BV（BV指树脂柱内装载树脂的体积）、洗脱剂为体积分数80%的乙醇溶液、洗脱流速1.0 m L/min,洗脱剂用量4 BV。所得洗脱液中黄酮质量分数从纯化前10.20%增加到纯化后51.89%,回收率为84.43%。体外实验表明,纯化后的黄酮可以清除羟自由基和超氧阴离子自由基,并有较好的还原力。纯化后的黄酮可以作为一种潜在的天然抗氧化剂。
