This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targ...This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.展开更多
The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α(HIF-1α)were investigated.The Hela cells were divided into three groups,namely,H0(no...The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α(HIF-1α)were investigated.The Hela cells were divided into three groups,namely,H0(non-transfected Hela cells),H1(pGenesil-1 empty plasmid-transfected Hela cells),and H2(HIF-1α-shRNA plasmid-transfected Hela cells),and were cultured under hypoxia(1%O2)and normoxia for 48 h.The expression of HIF-1α,E-cadherin,β-catenin,and actin was detected using Western blot.The scratch test and the invasion assay were applied to examine the invasion in each group.The expression of HIF-1α,E-cadherin,andβ-catenin in tumor grafts was assayed immunohistochemically.Western blot results revealed that the bands of HIF-1α,E-cadherin,β-catenin,and actin proteins were detected in the H0 and H1 groups under hypoxia for 48 h.The expression of E-cadherin,β-catenin,and actin was detected in the H2 group under hypoxia for 48 h,and normoxia.In the H0,H1,and H2 groups under normoxia,and the H2 group under hypoxia for 48 h,no expression of HIF-1αwas detectable.The scratch test showed that the invasive ability in the H2 group was significantly alleviated.Immunohistochemi-cally,it was found that there was a significant difference in the expression of HIF-1α,E-cadherin,andβ-catenin between the H1 and H2 groups(P<0.05),but the difference was not significant between the H0 and H1 groups.It was concluded that the effects of hypoxia on the invasion of human cervical cancer Hela cells and the expression of related proteins(E-cadherin,β-catenin,and actin)depend on HIF-1α.展开更多
基金supported by the National Natural Science Foundation of China,No.81272986the Natural Science Foundation of Shandong Province,No.ZR2011HZ002
文摘This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.
基金supported by the Key Science Research Foundation of Health Department of Hubei Province(No.JX1A06).
文摘The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α(HIF-1α)were investigated.The Hela cells were divided into three groups,namely,H0(non-transfected Hela cells),H1(pGenesil-1 empty plasmid-transfected Hela cells),and H2(HIF-1α-shRNA plasmid-transfected Hela cells),and were cultured under hypoxia(1%O2)and normoxia for 48 h.The expression of HIF-1α,E-cadherin,β-catenin,and actin was detected using Western blot.The scratch test and the invasion assay were applied to examine the invasion in each group.The expression of HIF-1α,E-cadherin,andβ-catenin in tumor grafts was assayed immunohistochemically.Western blot results revealed that the bands of HIF-1α,E-cadherin,β-catenin,and actin proteins were detected in the H0 and H1 groups under hypoxia for 48 h.The expression of E-cadherin,β-catenin,and actin was detected in the H2 group under hypoxia for 48 h,and normoxia.In the H0,H1,and H2 groups under normoxia,and the H2 group under hypoxia for 48 h,no expression of HIF-1αwas detectable.The scratch test showed that the invasive ability in the H2 group was significantly alleviated.Immunohistochemi-cally,it was found that there was a significant difference in the expression of HIF-1α,E-cadherin,andβ-catenin between the H1 and H2 groups(P<0.05),but the difference was not significant between the H0 and H1 groups.It was concluded that the effects of hypoxia on the invasion of human cervical cancer Hela cells and the expression of related proteins(E-cadherin,β-catenin,and actin)depend on HIF-1α.
