: Molecular markers were used to identify and assess cultivars of Laminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After scree...: Molecular markers were used to identify and assess cultivars of Laminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After screening, 11 primers were selected and they yielded 133 bands in all, of which approximately 99.2% were polymorphic. The genetic distances between gametophytes ranged from 0.412 to 0.956. Two clusters were formed with the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the simple matching coefficient. All cultivars of Laminaria japonica Aresch. used for breeding in China fell into one cluster. L. japonica from Japan, L. saccharina (L.) Lam., and L. angustata Kjellm. formed the other cluster and showed higher genetic variation than L. japonica from China. Nuclear ribosomal DNA (rDNA) sequences, including internal transcribed spacers (ITS 1 and ITS2) were studied and aligned. The nucleotides of the sequences ranged from 634 to 668, with a total of 692 positions including ITS1, ITS2, and the 5.8S coding region. The phylogenetic tree obtained by the neighbor-joining method favored, to some extent, the results revealed by RAPD analysis. The present study indicates that RAPD and ITS analyses could be used to identify and assess Laminaria germplasm and to distinguish some species and, even intraspecies, in Laminaria.展开更多
以茂名野生动物园斑鼻羚体内分离出的毛首线虫为研究对象,用保守引物PCR扩增其核糖体DNA（rDNA）的内转录间隔区（ITS）和5.8 S序列,并进行克隆、转化、测序和序列分析,对样品进行分子鉴定。结果获得2个毛首线虫样品的ITS及5.8 S rDNA序...以茂名野生动物园斑鼻羚体内分离出的毛首线虫为研究对象,用保守引物PCR扩增其核糖体DNA（rDNA）的内转录间隔区（ITS）和5.8 S序列,并进行克隆、转化、测序和序列分析,对样品进行分子鉴定。结果获得2个毛首线虫样品的ITS及5.8 S rDNA序列,总长为1 316 bp,样品间序列相似性为99.2%。将序列与GenBankTM公布的相关序列进行比较分析,结果显示与羊毛首线虫的ITS1、5.8 S和ITS2序列相似性高,分别为97.3%-97.6%、100%和97.8%-98.0%,表明斑鼻羚体内分离的毛首线虫属于羊毛首线虫。展开更多
从山东肥城采集到香菇病害样品,分离得到病原菌分离物FC,提取其基因组总DNA,利用真菌通用引物ITS1/ITS4扩增其r DNA ITS区域序列并进行测序。将获得的序列进行同源性比对和构建系统进化树,发现FC分离物与Gen Bank中8个米根霉(R.oryzae)...从山东肥城采集到香菇病害样品,分离得到病原菌分离物FC,提取其基因组总DNA,利用真菌通用引物ITS1/ITS4扩增其r DNA ITS区域序列并进行测序。将获得的序列进行同源性比对和构建系统进化树,发现FC分离物与Gen Bank中8个米根霉(R.oryzae)分离物同源性最高,为98.27%,结合形态学特征,证明该分离物为米根霉(R.oryzae)。展开更多
文摘: Molecular markers were used to identify and assess cultivars of Laminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After screening, 11 primers were selected and they yielded 133 bands in all, of which approximately 99.2% were polymorphic. The genetic distances between gametophytes ranged from 0.412 to 0.956. Two clusters were formed with the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the simple matching coefficient. All cultivars of Laminaria japonica Aresch. used for breeding in China fell into one cluster. L. japonica from Japan, L. saccharina (L.) Lam., and L. angustata Kjellm. formed the other cluster and showed higher genetic variation than L. japonica from China. Nuclear ribosomal DNA (rDNA) sequences, including internal transcribed spacers (ITS 1 and ITS2) were studied and aligned. The nucleotides of the sequences ranged from 634 to 668, with a total of 692 positions including ITS1, ITS2, and the 5.8S coding region. The phylogenetic tree obtained by the neighbor-joining method favored, to some extent, the results revealed by RAPD analysis. The present study indicates that RAPD and ITS analyses could be used to identify and assess Laminaria germplasm and to distinguish some species and, even intraspecies, in Laminaria.
文摘从山东肥城采集到香菇病害样品,分离得到病原菌分离物FC,提取其基因组总DNA,利用真菌通用引物ITS1/ITS4扩增其r DNA ITS区域序列并进行测序。将获得的序列进行同源性比对和构建系统进化树,发现FC分离物与Gen Bank中8个米根霉(R.oryzae)分离物同源性最高,为98.27%,结合形态学特征,证明该分离物为米根霉(R.oryzae)。
