目的:观察鸦胆子油乳剂联合白细胞介素-2腹腔内注射治疗恶性腹腔积液的疗效和不良反应。方法:对44例恶性腹腔积液患者采用腹腔放置引流管放净腹腔积液或腹腔排净腹腔积液后,分为两组,每组22例,联合用药组腹腔内每次注入鸦胆子油乳剂80～...目的:观察鸦胆子油乳剂联合白细胞介素-2腹腔内注射治疗恶性腹腔积液的疗效和不良反应。方法:对44例恶性腹腔积液患者采用腹腔放置引流管放净腹腔积液或腹腔排净腹腔积液后,分为两组,每组22例,联合用药组腹腔内每次注入鸦胆子油乳剂80～100 ml,白细胞介素-2300万～500万单位,单药组腹腔内注入鸦胆子油乳剂80～100ml,5～7 d 1次,最多4次为1个疗程,1个月后观察疗效、生活质量和不良反应。结果:联合用药组治疗恶性腹腔积液的有效率为90.90%(20/22),不良反应为发热(<38.5℃)19例,胃肠道反应1例,腹痛3例。单药组有效率为59.09%(12/22),不良反应为发热(<38.5℃)2例,胃肠道反应2例,腹痛1例。结论:鸦胆子油乳剂联合白细胞介素-2治疗恶性腹腔积液比单用鸦胆子油乳剂近期疗效、生活质量明显提高(P<0.05),不良反应给予对症处理后很快缓解,值得进一步推广使用。展开更多
Recombinant adenoviruses encoding murine IL 2 gene or IL 3 gene were directly injected into established subcutaneous tumor model of G422 glioblastoma cells. After treatment, the tumor size and survival of the gliobl...Recombinant adenoviruses encoding murine IL 2 gene or IL 3 gene were directly injected into established subcutaneous tumor model of G422 glioblastoma cells. After treatment, the tumor size and survival of the glioblastoma bearing mice were observed. The splenic NK and CTL cytotoxicities were detected by standard 4 hour 51 Cr release assay. We also examined the histopathological changes of tumor by hematoxylin and eosin staining. The results showed that intratumoral injection of adenoviruses encoding murine IL 2 gene or IL 3 gene significantly inhibited the growth of G422 glioblastoma and prolonged the survival period of glioblastoma bearing mice. The CTL cytotoxicity of the gene therapy groups was significantly higher than that of the control groups, but NK activity remained unchanged, indicating that specific immunity contributes to the in vivo antitumor effect of the direct gene therapy. There were much more tumor necrosis and inflammatory cell infiltration in the tumor of the gene therapy groups. Combined IL 2/IL 3 gene therapy could induce higher level of CTL and enhance the therapeutic potential further. The results suggest that intratumoral injection of recombinant adenoviruses encoding certain kind of cytokines may be a useful approach in the treatment of a malignancy of the central nervous system.展开更多
Panaxatriol ginsenoside (PTGS) is the major element of ginsenoside. It was shown that ginsenoside could regulate the functions of several immune cells, possibly mediated by enhanced secretion of interleukine-2 (IL-2) ...Panaxatriol ginsenoside (PTGS) is the major element of ginsenoside. It was shown that ginsenoside could regulate the functions of several immune cells, possibly mediated by enhanced secretion of interleukine-2 (IL-2) and interferon (IFN) . Recently, it was shown that the promotion of cytokines (IL-1, 2, 3, 4, 5, 6, IFN)with PTGS was related展开更多
文摘目的:观察鸦胆子油乳剂联合白细胞介素-2腹腔内注射治疗恶性腹腔积液的疗效和不良反应。方法:对44例恶性腹腔积液患者采用腹腔放置引流管放净腹腔积液或腹腔排净腹腔积液后,分为两组,每组22例,联合用药组腹腔内每次注入鸦胆子油乳剂80～100 ml,白细胞介素-2300万～500万单位,单药组腹腔内注入鸦胆子油乳剂80～100ml,5～7 d 1次,最多4次为1个疗程,1个月后观察疗效、生活质量和不良反应。结果:联合用药组治疗恶性腹腔积液的有效率为90.90%(20/22),不良反应为发热(<38.5℃)19例,胃肠道反应1例,腹痛3例。单药组有效率为59.09%(12/22),不良反应为发热(<38.5℃)2例,胃肠道反应2例,腹痛1例。结论:鸦胆子油乳剂联合白细胞介素-2治疗恶性腹腔积液比单用鸦胆子油乳剂近期疗效、生活质量明显提高(P<0.05),不良反应给予对症处理后很快缓解,值得进一步推广使用。
文摘Recombinant adenoviruses encoding murine IL 2 gene or IL 3 gene were directly injected into established subcutaneous tumor model of G422 glioblastoma cells. After treatment, the tumor size and survival of the glioblastoma bearing mice were observed. The splenic NK and CTL cytotoxicities were detected by standard 4 hour 51 Cr release assay. We also examined the histopathological changes of tumor by hematoxylin and eosin staining. The results showed that intratumoral injection of adenoviruses encoding murine IL 2 gene or IL 3 gene significantly inhibited the growth of G422 glioblastoma and prolonged the survival period of glioblastoma bearing mice. The CTL cytotoxicity of the gene therapy groups was significantly higher than that of the control groups, but NK activity remained unchanged, indicating that specific immunity contributes to the in vivo antitumor effect of the direct gene therapy. There were much more tumor necrosis and inflammatory cell infiltration in the tumor of the gene therapy groups. Combined IL 2/IL 3 gene therapy could induce higher level of CTL and enhance the therapeutic potential further. The results suggest that intratumoral injection of recombinant adenoviruses encoding certain kind of cytokines may be a useful approach in the treatment of a malignancy of the central nervous system.
文摘Panaxatriol ginsenoside (PTGS) is the major element of ginsenoside. It was shown that ginsenoside could regulate the functions of several immune cells, possibly mediated by enhanced secretion of interleukine-2 (IL-2) and interferon (IFN) . Recently, it was shown that the promotion of cytokines (IL-1, 2, 3, 4, 5, 6, IFN)with PTGS was related
