目的观察拉贝洛尔、硝苯地平联合硫酸镁治疗妊娠高血压的效果及对胰岛素样生长因子-Ⅰ(IGF-Ⅰ)、胰岛素样生长因子-Ⅱ(IGF-Ⅱ)、胰岛素样生长因子结合蛋白-1(IGFBP-1)的影响。方法选取2018年11月至2020年11月福建省立医院南院妇产科收治...目的观察拉贝洛尔、硝苯地平联合硫酸镁治疗妊娠高血压的效果及对胰岛素样生长因子-Ⅰ(IGF-Ⅰ)、胰岛素样生长因子-Ⅱ(IGF-Ⅱ)、胰岛素样生长因子结合蛋白-1(IGFBP-1)的影响。方法选取2018年11月至2020年11月福建省立医院南院妇产科收治的127例妊娠高血压患者,按照随机数表法分为对照组(n=63)和研究组(n=64),对照组使用硝苯地平+硫酸镁治疗,研究组使用拉贝洛尔+硫酸镁治疗,4周后比较两组的血压指标和生化指标,分娩后比较两组的胰岛素样生长因子水平、顺产率、不良结局发生率。结果研究组患者的收缩压(SBP)、舒张压(DBP)低于对照组,差异有统计学意义(P<0.05)。研究组患者的24 h尿蛋白水平(24 h rUprV)、B型钠尿肽(BNP)低于对照组,差异有统计学意义(P<0.05)。研究组产妇的IGF-Ⅰ、IGF-Ⅱ水平高于对照组,IGFBP-1低于对照组,差异有统计学意义(P<0.05)。研究组产妇的顺产率高于对照组,两组的分级数据比较,差异有统计学意义(P<0.05)。研究组的不良结局总发生率低于对照组,差异有统计学意义(P<0.05)。结论拉贝洛尔联合硫酸镁治疗妊娠高血压,有利于降低血压水平,调节胰岛素样生长因子,改善生化指标和妊娠结局,具有更好的临床治疗效果。展开更多
目的探讨单纯性生长激素缺乏症（isolated growth hormone deficiency, IGHD）以及特发性矮小症（idiopathic short stature，ISS）患儿经重组人生长激素（recombinant human growth hormone，rhGH）治疗后，血清C型利钠肽氨基末端（NTp...目的探讨单纯性生长激素缺乏症（isolated growth hormone deficiency, IGHD）以及特发性矮小症（idiopathic short stature，ISS）患儿经重组人生长激素（recombinant human growth hormone，rhGH）治疗后，血清C型利钠肽氨基末端（NTproCNP）浓度的变化及其与生长速率（growth velocity，GV）的关系。方法共有48例青春期前的患儿纳入研究（IGHD25例，ISS23例），并给予rhGH治疗1年。治疗前及治疗后6个月分别测血清胰岛素样生长因子-I（IGF—I）和NTproCNP的浓度。治疗1年后，计算所有患儿的GV、身高Z积分（HTSDS）以及身高Z积分的变化值（AHTSDS）。结果IGHD组中，治疗前后IGF—IZ积分的变化值（△IGF-ISDS）、NTproCNP浓度的变化值（△NTproCNP）与治疗1年中GV呈正相关（r=0．407，P=0．044；r=0．490，P=0．013）；治疗前生长激素（GH）峰值也与治疗前IGF—ISDS、NTproCNP浓度（r=0．558，P=0．004；r=0．630，P=0．001）以及治疗后△IGF-ISDS与△NTproCNP呈正相关（r=0．466，P=0．019）。而在ISS患儿中，治疗1年中GV只与治疗后△NTproCNP相关（r=0．845，P〈0．01）。结论在IGHD和ISS患儿应用rhGH的促生长治疗中，NTproCNP水平随着生长速率的增加而增加。因此除了IGF-I，NTproCNP作为一种新的生化标记物，也可用于评估和预测这两类患儿在rhGH治疗后的GV变化。展开更多
To express human insulin-like growth factor-I (hIGF-I) in transformed Bombyx mori cultured cells and silk glands, the transgenic vector pigA3GFP-hIGF-ie-neo was constructed with a neomycin resistance gene driven by th...To express human insulin-like growth factor-I (hIGF-I) in transformed Bombyx mori cultured cells and silk glands, the transgenic vector pigA3GFP-hIGF-ie-neo was constructed with a neomycin resistance gene driven by the baculovirus ie-1 promoter, and with the hIGF-I gene under the control of the silkworm sericin promoter Ser-1. The stably transformed BmN cells expressing hIGF-I were selected by using the antibiotic G418 at a final concentration of 700-800 μg/mL after the BmN cells were transfected with the piggyBac vector and the helper plasmid. The specific band of hIGF-I was detected in the transformed cells by Western blot. The expression level of hIGF-I, determined by ELISA, was about 7800 pg in 5×105 cells. Analysis of the chromosomal insertion sites by inverse PCR showed that exogenous DNA could be inserted into the cell genome randomly or at TTAA target sequence specifically for piggyBac element transposition. The transgenic vector pigA3GFP-hIGF-ie-neo was transferred into the eggs using sperm-mediated gene transfer. Finally, two transgenic silkworms were obtained after screening for the neo and gfp genes and verified by PCR and dot hybridization. The expression level of hIGF-I determined by ELISA was about 2440 pg/g of silk gland of the transgenic silkworms of the G1 generation.展开更多
文摘目的观察拉贝洛尔、硝苯地平联合硫酸镁治疗妊娠高血压的效果及对胰岛素样生长因子-Ⅰ(IGF-Ⅰ)、胰岛素样生长因子-Ⅱ(IGF-Ⅱ)、胰岛素样生长因子结合蛋白-1(IGFBP-1)的影响。方法选取2018年11月至2020年11月福建省立医院南院妇产科收治的127例妊娠高血压患者,按照随机数表法分为对照组(n=63)和研究组(n=64),对照组使用硝苯地平+硫酸镁治疗,研究组使用拉贝洛尔+硫酸镁治疗,4周后比较两组的血压指标和生化指标,分娩后比较两组的胰岛素样生长因子水平、顺产率、不良结局发生率。结果研究组患者的收缩压(SBP)、舒张压(DBP)低于对照组,差异有统计学意义(P<0.05)。研究组患者的24 h尿蛋白水平(24 h rUprV)、B型钠尿肽(BNP)低于对照组,差异有统计学意义(P<0.05)。研究组产妇的IGF-Ⅰ、IGF-Ⅱ水平高于对照组,IGFBP-1低于对照组,差异有统计学意义(P<0.05)。研究组产妇的顺产率高于对照组,两组的分级数据比较,差异有统计学意义(P<0.05)。研究组的不良结局总发生率低于对照组,差异有统计学意义(P<0.05)。结论拉贝洛尔联合硫酸镁治疗妊娠高血压,有利于降低血压水平,调节胰岛素样生长因子,改善生化指标和妊娠结局,具有更好的临床治疗效果。
基金Supported by the National Basic Research Program of China (Grant No. 2005CB121000)the National Natural Science Foundation of China (Grant No. 30671590)
文摘To express human insulin-like growth factor-I (hIGF-I) in transformed Bombyx mori cultured cells and silk glands, the transgenic vector pigA3GFP-hIGF-ie-neo was constructed with a neomycin resistance gene driven by the baculovirus ie-1 promoter, and with the hIGF-I gene under the control of the silkworm sericin promoter Ser-1. The stably transformed BmN cells expressing hIGF-I were selected by using the antibiotic G418 at a final concentration of 700-800 μg/mL after the BmN cells were transfected with the piggyBac vector and the helper plasmid. The specific band of hIGF-I was detected in the transformed cells by Western blot. The expression level of hIGF-I, determined by ELISA, was about 7800 pg in 5×105 cells. Analysis of the chromosomal insertion sites by inverse PCR showed that exogenous DNA could be inserted into the cell genome randomly or at TTAA target sequence specifically for piggyBac element transposition. The transgenic vector pigA3GFP-hIGF-ie-neo was transferred into the eggs using sperm-mediated gene transfer. Finally, two transgenic silkworms were obtained after screening for the neo and gfp genes and verified by PCR and dot hybridization. The expression level of hIGF-I determined by ELISA was about 2440 pg/g of silk gland of the transgenic silkworms of the G1 generation.
