目的观察粗针督脉平刺对缺血性面瘫大鼠的治疗效果,初步探讨产生疗效的可能机制。方法将6 0只W i s t a r大鼠随机分为A组(粗针平刺组)、B组(基础西药组)、C组(空白对照组)和D组(假手术组),每组1 5只。所有大鼠均采用改良血管栓塞法建...目的观察粗针督脉平刺对缺血性面瘫大鼠的治疗效果,初步探讨产生疗效的可能机制。方法将6 0只W i s t a r大鼠随机分为A组(粗针平刺组)、B组(基础西药组)、C组(空白对照组)和D组(假手术组),每组1 5只。所有大鼠均采用改良血管栓塞法建立缺血性面瘫模型。在治疗期间对各组大鼠进行面神经功能缺损评分,A组于造模后1 d选取神道穴平刺,留针4 h,每日1次,共治疗1 4 d;B组于造模后1 d予强的松灌胃、维生素B1 2腹腔注射治疗,每日1次,共治疗1 4 d;C组与D组不进行治疗。分别在造模后第3、7、1 4天随机选取4只,采用免疫组织化学法进行面神经伴行固有血管组织H I F-1α蛋白测定,酶联免疫吸附测定法检测血清N O,放射免疫法检测血清E T含量。结果治疗7 d后,A组和B组神经功能缺损评分逐渐升高,与同组造模后比较,差异均具有统计学意义(P<0.0 1)。A组和B组治疗7 d后神经功能缺损评分与C组和D组比较,差异均具有统计学意义(P<0.0 5)。C组治疗7 d后神经功能缺损评分与D组比较,差异具有统计学意义(P<0.0 5)。A组和B组治疗1 4 d后神经功能缺损评分与C组比较,差异均具有统计学意义(P<0.0 5)。B组和C组治疗1 4 d后神经功能缺损评分与D组比较,差异均具有统计学意义(P<0.0 5)。A组治疗1 4 d后神经功能缺损评分与同组治疗7 d后相比较,差异具有统计学意义(P<0.0 5)。A组、B组和C组治疗3、7、1 4 d后H I F-1αM O D值与D组比较,差异均具有统计学意义(P<0.0 1)。A组和B组治疗3、7 d后H I F-1αM O D值与C组比较,差异均具有统计学意义(P<0.0 5)。A组和B组治疗3、1 4 d后H I F-1αM O D值与同组治疗7 d后比较,差异均具有统计学意义(P<0.0 5)。A组和B组治疗3、7 d后血清N O含量与D组比较,差异均具有统计学意义(P<0.0 1)。A组和B组治疗7 d后血清N O含量与同组治疗3 d后比较,差异均具有统计学意义(P<0.0 5)。A组、B组和C组治疗3、7 展开更多
AIM: To investigate the effects of 1400W-a selective inducible nitric oxide synthase(iN OS) inhibitor in a model of donation after circulatory death(DCD) kidneys. METHODS: Porcine kidneys were retrieved after 25 min w...AIM: To investigate the effects of 1400W-a selective inducible nitric oxide synthase(iN OS) inhibitor in a model of donation after circulatory death(DCD) kidneys. METHODS: Porcine kidneys were retrieved after 25 min warm ischemia. They were then stored on ice for 18 h before being reperfused ex vivo with oxygenated autologous blood on an isolated organ perfusion system. The selective i NOS inhibitor 1400W(10 mg/kg) was administered before reperfusion(n = 6) vs control group(n = 7). Creatinine(1000 μmol/L) was added to the system, renal and tubular cell function and the level of ischemia reperfusion injury were assessed over 3 h of reperfusion using plasma, urine and tissue samples. RESULTS: Kidneys treated with 1400 W had a higher level of creatinine clearance(CrC l) [area under the curve(AUC) CrC l: 2.37 ± 0.97 mL /min per 100 g vs 0.96 ± 0.32 mL /min per 100 g, P = 0.004] and urine output [Total: 320 ± 96 mL vs 156 ± 82 mL, P = 0.008]. There was no significant difference in levels of fractional excretion of sodium(AUC, Fr ex Na+: Control, 186.3% ± 81.7%.h vs 1400 W, 153.4% ± 12.1%.h, P = 0.429). Levels of total protein creatinine ratio were significantly lower in the 1400 W group after 1 h of reperfusion(1h Pr/Cr: 1400 W 9068 ± 6910 mg/L/mmol/L vs Control 21586 ± 5464 mg/L/mmol/L, P = 0.026). Levels of 8-isoprostane were significantly lower in the 1400 W group [8-iso/creatinine ratio: Control 239 ± 136 pg/L/mmol/L vs 1400 W 139 ± 47 pg/L/mmol/L, P = 0.041].CONCLUSION: This study demonstrated that 1400 W reduced ischaemia reperfusion injury in this porcine kidney model of DCD donor. Kidneys had improved renal function and reduced oxidative stress.展开更多
目的观察各组大鼠前额叶皮质区神经元形态学损伤及该区诱导型一氧化氮合成酶的表达量,探究羟基红花黄素A(HSYA)对淋巴滞留性脑病(LE)大鼠的作用。方法 63只Wistar大鼠随机分为假手术组(SHAM组)、HSYA组和LE组,每组分为1、7、14 d 3个子...目的观察各组大鼠前额叶皮质区神经元形态学损伤及该区诱导型一氧化氮合成酶的表达量,探究羟基红花黄素A(HSYA)对淋巴滞留性脑病(LE)大鼠的作用。方法 63只Wistar大鼠随机分为假手术组(SHAM组)、HSYA组和LE组,每组分为1、7、14 d 3个子组。HSYA组和LE组行结扎并摘除颈部淋巴结操作;HSYA组手术当天和术后腹腔注射给药,SHAM组和LE组给予等量生理盐水。3组大鼠于术后第1、7、14天各取5只行多聚甲醛灌心取前额叶皮质区脑组织制作石蜡切片,并行苏木精-伊红染色和免疫组织化学染色,观察各组前额叶皮质区神经元形态学改变和诱导型一氧化氮合成酶表达量;同时,每组取2只大鼠的前额叶皮质区脑组织,制片进行扫描电镜观察。结果光学显微镜下可见LE组神经细胞形态不规则,核染色加深、核膜破裂,其他两组未见明显异常;免疫组织化学染色切片显示,LE组一氧化氮合成酶表达量显著上升,术后1 d组表达量最大,以后逐渐下降,并分别与另两组的1 d子组相比差异有统计学意义(P<0.05);扫描电镜下可见LE组核仁消失、髓鞘断裂等,其他两组未见上述改变。结论淋巴液的脑内滞留诱发前额叶皮质区神经细胞合成大量诱导型一氧化氮合成酶,可导致神经细胞形态的损伤,HSYA可以降低该区诱导型一氧化氮合成酶的表达,并减轻神经元损伤程度。展开更多
Objective To investigate the effect of apoptosis and inducible nitric oxide (iNOS) on the early spontaneous abortion Methods TUNEL method was used to detect the apoptosis in trophoblast cells in early pregnancy wi...Objective To investigate the effect of apoptosis and inducible nitric oxide (iNOS) on the early spontaneous abortion Methods TUNEL method was used to detect the apoptosis in trophoblast cells in early pregnancy with and without spontaneous abortion (the experiment group and the control group), while iNOS was detected by both in situ hybridization and immunohistochemistry. By computer color magic image analysis system(CMIAS), positive cell indexes were represented by D (density) and N/S(number/square) in both apoptosis and in situ hybridization, in immunohistochemistry were N/S and PU(positive unit). Results Positive cell indexes of apoptosis D and N/S were significntly higher in the experiment group (0.48±0.004, 0.045±0.002) than that in the control group(0.35+0.06, 0.031±0.003. P<0.001). D and N/S of inducible nitric oxide synthase in situ hybridization were 0.33±0.028, 0.074±0.001 respectively in the experiment group and 0.13±0.015, 0.019±0.004 respectively in the control group. N/S and PU were significantly higher in the experiment group(0.058±0.007, 11.94±2.01) than that in the control group (0.007±0.001, 1.18±0.35, P<0.01). There existed a positive correlation between iNOS and apoptosis too. Conclution Apoptosis and iNOS in trophoblasts might play an important role in early spontaneous abortion and there was a positive correlation between apoptosis and iNOS.展开更多
In order to study the effect of nitric oxide (NO) on the expression of hypoxia inducible factor 1 alpha (HIF 1α) mRNA in hypoxic pulmonary hypertension (HPH) rats, 30 healthy male Wistar rats were randomly divide...In order to study the effect of nitric oxide (NO) on the expression of hypoxia inducible factor 1 alpha (HIF 1α) mRNA in hypoxic pulmonary hypertension (HPH) rats, 30 healthy male Wistar rats were randomly divided into normoxic control group, chronic hypoxic group and hypoxia plus L argine (L Arg) group. The animal model of HPH was developed. The mean pulmonary arterial pressure (mPAP) was measured by inserting a microcatheter into the pulmonary artery. The HIF 1α mRNA expression levels were detected by in situ hybridization (ISH) and semiquantitative RT PCR. It was found that after 14 days hypoxia, the mPAP in normoxic control group (17.6±2 7 mmHg,1 mmHg=0 133 kPa) was significantly lower than that in chronic hypoxic group(35.8±6.1 mmHg, t =0.2918, P <0.05) and mPAP in chronic hypoxic group was higher than that in hypoxia plus L argine group(24.4±3.8 mmHg, t =0.2563, P <0.05). ISH showed that the expression of HIF 1α mRNA in the intraacinar pulmonary arteriolae (IAPA) in normoxic control group (0.1076±0.0205) was markedly weaker than that in chronic hypoxic group (0.3317±0.0683, t =3.125, P <0.05) and that in chronic hypoxic group was stronger than that in hypoxia plus L argine group (0.1928±0.0381, t =2.844, P <0.05). RT PCR showed that the content of HIF 1α mRNA in chronic hypoxic group (2.5395±0.6449) was 2.16 times and 1.75 times higher than that in normoxic control group (1.1781±0.3628) and hypoxia plus L argine group (1.4511±0.3981), respectively. It is concluded that NO can reduce the mPAP by the inhibition of the expression of HIF 1α mRNA, which may be one of the mechanisms through which NO affects the pathogenesis of HPH.展开更多
目的:探讨诱导型一氧化氮合酶(iNOS)在糖尿病大鼠视网膜组织中的表达及其对细胞凋亡的影响。方法:建立糖尿病大鼠动物模型,分别在成模后第1,3,6mo时测定大鼠视网膜组织细胞凋亡指数(AI)和iNOS表达水平。结果:(1)与正常对照组(NC)相比,i...目的:探讨诱导型一氧化氮合酶(iNOS)在糖尿病大鼠视网膜组织中的表达及其对细胞凋亡的影响。方法:建立糖尿病大鼠动物模型,分别在成模后第1,3,6mo时测定大鼠视网膜组织细胞凋亡指数(AI)和iNOS表达水平。结果:(1)与正常对照组(NC)相比,iNOS表达在糖尿病各组均升高,6mo时明显增强(P<0.05);(2)视网膜细胞凋亡情况:与正常对照组(NC)相比,糖尿病模型1mo时细胞凋亡指数差异无统计学意义,3,6mo时则明显升高(P<0.05)。结论:iNOS在糖尿病大鼠视网膜组织中的表达增强,推断iNOS在糖尿病视网膜病变(retinopathy of diabetes,DR)的发病机制中起一定作用。展开更多
文摘AIM: To investigate the effects of 1400W-a selective inducible nitric oxide synthase(iN OS) inhibitor in a model of donation after circulatory death(DCD) kidneys. METHODS: Porcine kidneys were retrieved after 25 min warm ischemia. They were then stored on ice for 18 h before being reperfused ex vivo with oxygenated autologous blood on an isolated organ perfusion system. The selective i NOS inhibitor 1400W(10 mg/kg) was administered before reperfusion(n = 6) vs control group(n = 7). Creatinine(1000 μmol/L) was added to the system, renal and tubular cell function and the level of ischemia reperfusion injury were assessed over 3 h of reperfusion using plasma, urine and tissue samples. RESULTS: Kidneys treated with 1400 W had a higher level of creatinine clearance(CrC l) [area under the curve(AUC) CrC l: 2.37 ± 0.97 mL /min per 100 g vs 0.96 ± 0.32 mL /min per 100 g, P = 0.004] and urine output [Total: 320 ± 96 mL vs 156 ± 82 mL, P = 0.008]. There was no significant difference in levels of fractional excretion of sodium(AUC, Fr ex Na+: Control, 186.3% ± 81.7%.h vs 1400 W, 153.4% ± 12.1%.h, P = 0.429). Levels of total protein creatinine ratio were significantly lower in the 1400 W group after 1 h of reperfusion(1h Pr/Cr: 1400 W 9068 ± 6910 mg/L/mmol/L vs Control 21586 ± 5464 mg/L/mmol/L, P = 0.026). Levels of 8-isoprostane were significantly lower in the 1400 W group [8-iso/creatinine ratio: Control 239 ± 136 pg/L/mmol/L vs 1400 W 139 ± 47 pg/L/mmol/L, P = 0.041].CONCLUSION: This study demonstrated that 1400 W reduced ischaemia reperfusion injury in this porcine kidney model of DCD donor. Kidneys had improved renal function and reduced oxidative stress.
文摘Objective To investigate the effect of apoptosis and inducible nitric oxide (iNOS) on the early spontaneous abortion Methods TUNEL method was used to detect the apoptosis in trophoblast cells in early pregnancy with and without spontaneous abortion (the experiment group and the control group), while iNOS was detected by both in situ hybridization and immunohistochemistry. By computer color magic image analysis system(CMIAS), positive cell indexes were represented by D (density) and N/S(number/square) in both apoptosis and in situ hybridization, in immunohistochemistry were N/S and PU(positive unit). Results Positive cell indexes of apoptosis D and N/S were significntly higher in the experiment group (0.48±0.004, 0.045±0.002) than that in the control group(0.35+0.06, 0.031±0.003. P<0.001). D and N/S of inducible nitric oxide synthase in situ hybridization were 0.33±0.028, 0.074±0.001 respectively in the experiment group and 0.13±0.015, 0.019±0.004 respectively in the control group. N/S and PU were significantly higher in the experiment group(0.058±0.007, 11.94±2.01) than that in the control group (0.007±0.001, 1.18±0.35, P<0.01). There existed a positive correlation between iNOS and apoptosis too. Conclution Apoptosis and iNOS in trophoblasts might play an important role in early spontaneous abortion and there was a positive correlation between apoptosis and iNOS.
文摘In order to study the effect of nitric oxide (NO) on the expression of hypoxia inducible factor 1 alpha (HIF 1α) mRNA in hypoxic pulmonary hypertension (HPH) rats, 30 healthy male Wistar rats were randomly divided into normoxic control group, chronic hypoxic group and hypoxia plus L argine (L Arg) group. The animal model of HPH was developed. The mean pulmonary arterial pressure (mPAP) was measured by inserting a microcatheter into the pulmonary artery. The HIF 1α mRNA expression levels were detected by in situ hybridization (ISH) and semiquantitative RT PCR. It was found that after 14 days hypoxia, the mPAP in normoxic control group (17.6±2 7 mmHg,1 mmHg=0 133 kPa) was significantly lower than that in chronic hypoxic group(35.8±6.1 mmHg, t =0.2918, P <0.05) and mPAP in chronic hypoxic group was higher than that in hypoxia plus L argine group(24.4±3.8 mmHg, t =0.2563, P <0.05). ISH showed that the expression of HIF 1α mRNA in the intraacinar pulmonary arteriolae (IAPA) in normoxic control group (0.1076±0.0205) was markedly weaker than that in chronic hypoxic group (0.3317±0.0683, t =3.125, P <0.05) and that in chronic hypoxic group was stronger than that in hypoxia plus L argine group (0.1928±0.0381, t =2.844, P <0.05). RT PCR showed that the content of HIF 1α mRNA in chronic hypoxic group (2.5395±0.6449) was 2.16 times and 1.75 times higher than that in normoxic control group (1.1781±0.3628) and hypoxia plus L argine group (1.4511±0.3981), respectively. It is concluded that NO can reduce the mPAP by the inhibition of the expression of HIF 1α mRNA, which may be one of the mechanisms through which NO affects the pathogenesis of HPH.
文摘目的:探讨诱导型一氧化氮合酶(iNOS)在糖尿病大鼠视网膜组织中的表达及其对细胞凋亡的影响。方法:建立糖尿病大鼠动物模型,分别在成模后第1,3,6mo时测定大鼠视网膜组织细胞凋亡指数(AI)和iNOS表达水平。结果:(1)与正常对照组(NC)相比,iNOS表达在糖尿病各组均升高,6mo时明显增强(P<0.05);(2)视网膜细胞凋亡情况:与正常对照组(NC)相比,糖尿病模型1mo时细胞凋亡指数差异无统计学意义,3,6mo时则明显升高(P<0.05)。结论:iNOS在糖尿病大鼠视网膜组织中的表达增强,推断iNOS在糖尿病视网膜病变(retinopathy of diabetes,DR)的发病机制中起一定作用。
