A monitoring system is an important guarantee of safety in a production mine. However, not all monitoring substations pass the electrical fast transient/burst （EFT/B） immunity test and the explosion-proof test simul...A monitoring system is an important guarantee of safety in a production mine. However, not all monitoring substations pass the electrical fast transient/burst （EFT/B） immunity test and the explosion-proof test simultaneously. To enhance the immunity, the values of capacitance and inductance should be increased, which are actually limited by coal mine explosion-proof standards. Hence, for the first time, an active filter was applied in an electromagnetic interference （EMI） output filter. As a result, the interference within 30 MHz clearly weakened, but the frequency spectrum had a wide range. An EMI input filter and ferrite beads were adopted to restrain higher frequency interference. An output interference spectrogram of the substation was obtained with an analyzer. The results indicate that the improved complex filtering markedly help to control interference. With the support of improved complex filtering and other enhancing immunity means about I/O ports, the substation managed to pass both the EFT/B immunity test and the explosion-proof test synchronously. We conclude that improved complex filtering is of vital importance in enhancing the electromagnetic compatibilitv （EMC） of the coal mine monitoring system.展开更多
Plant cells mount plenty of pattern-recognition receptors(PRRs)to detect the microbe-associated molecular patterns(MAMPs)from potential microbial pathogens.MAMPs are overrepresented by proteinaneous patterns,such as t...Plant cells mount plenty of pattern-recognition receptors(PRRs)to detect the microbe-associated molecular patterns(MAMPs)from potential microbial pathogens.MAMPs are overrepresented by proteinaneous patterns,such as the flg22 peptide from bacterial flagellin.Identification of PRR receptor complex components by forward or reverse genetics can be time/labor-consuming,and be confounded by functional redundancies.Here,we present a strategy for identifying PRR complex components by engineering plants to inducibly secrete affinity-tagged proteinaneous MAMPs to the apoplast.The PRR protein complexes bound to self-secreted MAMPs are enriched through affinity purification and dissected by mass spectrometry.As a proof of principle,we could capture the flg22 receptor FLS2 and co-receptor BAK1 using Arabidopsis plants secreting FLAG-tagged flg22 under estradiolinduction.Moreover,we identified receptor-like kinases LIK1 and PEPR1/PEPR2 as potential components in the FLS2 receptor complex,which were further validated by protein–protein interaction assays and the reverse genetics approach.Our study showcases a simple way to biochemically identify endogenous PRR complex components without overexpressing the PRR or using chemical crosslinkers,and suggests a possible crosstalk between different immune receptors in plants.A modest dose of estradiol can also be applied to inducing enhanced immunity in engineered plants to both bacterial and fungal pathogens.展开更多
The peptides mixture was prepared from tumor cells by freezing-thawing cells, precipitation by heating, followed by acidification of the solution. The activation and proliferation of mouse splenocytes by HSP70-peptide...The peptides mixture was prepared from tumor cells by freezing-thawing cells, precipitation by heating, followed by acidification of the solution. The activation and proliferation of mouse splenocytes by HSP70-peptide complex, formed by the binding of HSP70 and peptides in vitro, were observed, so was the specific cytotoxicity of the proliferative lymphocytes to tumor cells. The phenotypes of the proliferative lymphocytes were analyzed by a flow cytometer. BALB/c mice inoculated with H22 hepatocarcinoma cells in peritoneal cavity or hind thigh were immunized by injection with HSP70-peptides complex to observe the inhibitory effect of the immunization on tumor and lifetime of tumor-bearing mice. On the other hand, blood samples were collected from the immunized mice to check the functions of liver and kidney. The results showed that the peptides mixture from tumor cells contained tumor-specific antigen peptides which could be presented by HSP70 to activate lymphocytes in vitro, the proliferative lymphocytes were T cells which were specifically cytotoxic to tumor cells, the in vivo growth of both ascitic and solid carcinoma could be suppressed by immunization with HSP70-peptides and the lifetime of tumor-bearing mice was prolonged, the in vivo immunization with HSP70-H22-peptides had no impact on the function of mouse liver and kidney, suggesting that there was no occurrence of autoimmunity in vivo after immunization.展开更多
Olfactory ensheathing cells （OECs） can promote axonal regeneration and remyelination for the treatment of spinal cord injury. OECs can also treat experimental allergic encephalomyelitis （EAE）, but it remains uncle...Olfactory ensheathing cells （OECs） can promote axonal regeneration and remyelination for the treatment of spinal cord injury. OECs can also treat experimental allergic encephalomyelitis （EAE）, but it remains unclear whether OECs might be rejected by the immune system in the brain including the destruction of the blood-brain barrier under inflammation, the release of inflammatory factors, the activation of local antigen-presenting cells （e.g., microglia cells） and antigen drainage. We found that OECs expressed major histocompatibility complex （MHC）-I molecules on the cell surface, barely expressed MHC-II, but MHC-II could be induced by interferon-v, suggesting that OECs have certain immunogenicity. When OECs were transplanted into normal animal brains, no OECs were phagocytosed by dendritic cells in the cervical lymph node, and OECs did not induce lymphocyte proliferation, which indicates that OECs share some immune privilege under normal conditions. However, OECs in the rat EAE brain were phagocytosed by dendritic cells in the cervical lymph node and enhanced lymphocyte proliferation. These findings suggest that OECs are rejected because of increased immunogenicity in EAE brain, and that brain inflammation, in particular activated dendritic cells, may be a prerequisite for rejecting OECs.展开更多
We present two cases of pulmonary MAC infection in women with Hürthle cell thyroid carcinoma. Both cases were asymptomatic octogenarian women with active Hürthle cell thyroid carcinoma and prolonged periods ...We present two cases of pulmonary MAC infection in women with Hürthle cell thyroid carcinoma. Both cases were asymptomatic octogenarian women with active Hürthle cell thyroid carcinoma and prolonged periods of hypothyroidism prior to diagnosis of pulmonary MAC. Mycobacterium avium complex has never been reported in association with any type of thyroid cancer, specifically Hürthle cell carcinoma. A review of the literature and possible associations between the two are discussed in this article.展开更多
基金Project 50674093 supported by the National Natural Science Foundation of China
文摘A monitoring system is an important guarantee of safety in a production mine. However, not all monitoring substations pass the electrical fast transient/burst （EFT/B） immunity test and the explosion-proof test simultaneously. To enhance the immunity, the values of capacitance and inductance should be increased, which are actually limited by coal mine explosion-proof standards. Hence, for the first time, an active filter was applied in an electromagnetic interference （EMI） output filter. As a result, the interference within 30 MHz clearly weakened, but the frequency spectrum had a wide range. An EMI input filter and ferrite beads were adopted to restrain higher frequency interference. An output interference spectrogram of the substation was obtained with an analyzer. The results indicate that the improved complex filtering markedly help to control interference. With the support of improved complex filtering and other enhancing immunity means about I/O ports, the substation managed to pass both the EFT/B immunity test and the explosion-proof test synchronously. We conclude that improved complex filtering is of vital importance in enhancing the electromagnetic compatibilitv （EMC） of the coal mine monitoring system.
基金National Natural Science Foundation of China grants 31570276 and 31770295partially supported by Sun Yat-sen University(33000–31143406)。
文摘Plant cells mount plenty of pattern-recognition receptors(PRRs)to detect the microbe-associated molecular patterns(MAMPs)from potential microbial pathogens.MAMPs are overrepresented by proteinaneous patterns,such as the flg22 peptide from bacterial flagellin.Identification of PRR receptor complex components by forward or reverse genetics can be time/labor-consuming,and be confounded by functional redundancies.Here,we present a strategy for identifying PRR complex components by engineering plants to inducibly secrete affinity-tagged proteinaneous MAMPs to the apoplast.The PRR protein complexes bound to self-secreted MAMPs are enriched through affinity purification and dissected by mass spectrometry.As a proof of principle,we could capture the flg22 receptor FLS2 and co-receptor BAK1 using Arabidopsis plants secreting FLAG-tagged flg22 under estradiolinduction.Moreover,we identified receptor-like kinases LIK1 and PEPR1/PEPR2 as potential components in the FLS2 receptor complex,which were further validated by protein–protein interaction assays and the reverse genetics approach.Our study showcases a simple way to biochemically identify endogenous PRR complex components without overexpressing the PRR or using chemical crosslinkers,and suggests a possible crosstalk between different immune receptors in plants.A modest dose of estradiol can also be applied to inducing enhanced immunity in engineered plants to both bacterial and fungal pathogens.
基金This work was supported by the National Natural Science Foundation of China (Grant No.39970322) Trans-Century Training Programme Foundation for Talent under the supervision of Ministry of Education of China.
文摘The peptides mixture was prepared from tumor cells by freezing-thawing cells, precipitation by heating, followed by acidification of the solution. The activation and proliferation of mouse splenocytes by HSP70-peptide complex, formed by the binding of HSP70 and peptides in vitro, were observed, so was the specific cytotoxicity of the proliferative lymphocytes to tumor cells. The phenotypes of the proliferative lymphocytes were analyzed by a flow cytometer. BALB/c mice inoculated with H22 hepatocarcinoma cells in peritoneal cavity or hind thigh were immunized by injection with HSP70-peptides complex to observe the inhibitory effect of the immunization on tumor and lifetime of tumor-bearing mice. On the other hand, blood samples were collected from the immunized mice to check the functions of liver and kidney. The results showed that the peptides mixture from tumor cells contained tumor-specific antigen peptides which could be presented by HSP70 to activate lymphocytes in vitro, the proliferative lymphocytes were T cells which were specifically cytotoxic to tumor cells, the in vivo growth of both ascitic and solid carcinoma could be suppressed by immunization with HSP70-peptides and the lifetime of tumor-bearing mice was prolonged, the in vivo immunization with HSP70-H22-peptides had no impact on the function of mouse liver and kidney, suggesting that there was no occurrence of autoimmunity in vivo after immunization.
基金Young Talent Innovation Program of Fujian Province Department of Science and Technology,No.2006F3032
文摘Olfactory ensheathing cells （OECs） can promote axonal regeneration and remyelination for the treatment of spinal cord injury. OECs can also treat experimental allergic encephalomyelitis （EAE）, but it remains unclear whether OECs might be rejected by the immune system in the brain including the destruction of the blood-brain barrier under inflammation, the release of inflammatory factors, the activation of local antigen-presenting cells （e.g., microglia cells） and antigen drainage. We found that OECs expressed major histocompatibility complex （MHC）-I molecules on the cell surface, barely expressed MHC-II, but MHC-II could be induced by interferon-v, suggesting that OECs have certain immunogenicity. When OECs were transplanted into normal animal brains, no OECs were phagocytosed by dendritic cells in the cervical lymph node, and OECs did not induce lymphocyte proliferation, which indicates that OECs share some immune privilege under normal conditions. However, OECs in the rat EAE brain were phagocytosed by dendritic cells in the cervical lymph node and enhanced lymphocyte proliferation. These findings suggest that OECs are rejected because of increased immunogenicity in EAE brain, and that brain inflammation, in particular activated dendritic cells, may be a prerequisite for rejecting OECs.
文摘We present two cases of pulmonary MAC infection in women with Hürthle cell thyroid carcinoma. Both cases were asymptomatic octogenarian women with active Hürthle cell thyroid carcinoma and prolonged periods of hypothyroidism prior to diagnosis of pulmonary MAC. Mycobacterium avium complex has never been reported in association with any type of thyroid cancer, specifically Hürthle cell carcinoma. A review of the literature and possible associations between the two are discussed in this article.
