本研究将人生长激素基因重组 DNA 导入银鲫受精卵,或用人生长激素对60日龄银鲫连续6周腹腔注射,均观察到了受体鱼的快速生长效应,转移人生长激素基因鱼在60日龄时的平均体重比对照鱼平均体重增加82%,相应体长平均值比对照鱼增加19.7%;在...本研究将人生长激素基因重组 DNA 导入银鲫受精卵,或用人生长激素对60日龄银鲫连续6周腹腔注射,均观察到了受体鱼的快速生长效应,转移人生长激素基因鱼在60日龄时的平均体重比对照鱼平均体重增加82%,相应体长平均值比对照鱼增加19.7%;在125日龄时,转基因组平均体重比对照组增加17.7%,相应体长平均值比对照增加3.5%。定期注射人生长激素,在6周的注射实验过程中,实验鱼的体重,体长都与对照组相近或略有减小。但是,在水泥池中饲养一个月后,注射10/μg/g 体重的实验组鱼平均体重比相应对照组鱼的平均体重增加32%;相应体长平均值增加11%。展开更多
Korean spruce （Picea koraiensis Sieb. E1 Zucc.） is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement ...Korean spruce （Picea koraiensis Sieb. E1 Zucc.） is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine （P. sylvestris var. mongolica） seedlings brought by the plant beneficial fungus Trichoderma virens （J.H. Mill., Giddens and A.A. Foster） Arx and ectomycorrhizal fungus （Suillus luteus （L.） Roussel.） can also benefit Korean spruce seed- lings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -l,3-glucanase and chitinase. This inocu- lation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results.展开更多
Neurotrophins play a major role in the regulation of neuronal growth such as neurite sprouting or regeneration in response to nerve injuries. The role of nerve growth factor, neurotrophin-3, and brain-derived neurotro...Neurotrophins play a major role in the regulation of neuronal growth such as neurite sprouting or regeneration in response to nerve injuries. The role of nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor in maintaining the survival of peripheral neurons remains poorly understood. In regenerative medicine, different modalities have been investigated for the delivery of growth factors to the injured neurons, in search of a suitable system for clinical applications. This study was to investigate the influence of nerve growth factor, neurotrophin-3 and brain-derived neurotrophic factor on the growth of neurites using two in vitro models of dorsal root ganglia explants and dorsal root ganglia-derived primary cell dissociated cultures. Quantitative data showed that the total neurite length and tortuosity were differently influenced by trophic factors. Nerve growth factor and, indirectly, brain-derived neurotrophic factor stimulate the tortuous growth of sensory fibers and the formation of cell clusters. Neurotrophin-3, however, enhances neurite growth in terms of length and linearity allowing for a more organized and directed axonal elongation towards a peripheral target compared to the other growth factors. These findings could be of considerable importance for any clinical application of neurotrophic factors in peripheral nerve regeneration. Ethical approval was obtained from the Regione Piemonte Animal Ethics Committee ASLTO1(file # 864/2016-PR) on September 14, 2016.展开更多
文摘本研究将人生长激素基因重组 DNA 导入银鲫受精卵,或用人生长激素对60日龄银鲫连续6周腹腔注射,均观察到了受体鱼的快速生长效应,转移人生长激素基因鱼在60日龄时的平均体重比对照鱼平均体重增加82%,相应体长平均值比对照鱼增加19.7%;在125日龄时,转基因组平均体重比对照组增加17.7%,相应体长平均值比对照增加3.5%。定期注射人生长激素,在6周的注射实验过程中,实验鱼的体重,体长都与对照组相近或略有减小。但是,在水泥池中饲养一个月后,注射10/μg/g 体重的实验组鱼平均体重比相应对照组鱼的平均体重增加32%;相应体长平均值增加11%。
基金the National Natural Science Foundation of China (31170597, 31200484)the National Fiveyear Science and Technology Research Project (2012BAD19B0801)+1 种基金the Fundamental Research Funds for the Central Universities (2572014AA30)the National Forestry Bureau ‘‘948’’ Project (2009-4-39) for financial support
文摘Korean spruce （Picea koraiensis Sieb. E1 Zucc.） is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine （P. sylvestris var. mongolica） seedlings brought by the plant beneficial fungus Trichoderma virens （J.H. Mill., Giddens and A.A. Foster） Arx and ectomycorrhizal fungus （Suillus luteus （L.） Roussel.） can also benefit Korean spruce seed- lings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -l,3-glucanase and chitinase. This inocu- lation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results.
基金supported by the research start-up and the MWU’s intramural grant(to MF)the Italian MURST-MIUR foundation(to SG and IP)
文摘Neurotrophins play a major role in the regulation of neuronal growth such as neurite sprouting or regeneration in response to nerve injuries. The role of nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor in maintaining the survival of peripheral neurons remains poorly understood. In regenerative medicine, different modalities have been investigated for the delivery of growth factors to the injured neurons, in search of a suitable system for clinical applications. This study was to investigate the influence of nerve growth factor, neurotrophin-3 and brain-derived neurotrophic factor on the growth of neurites using two in vitro models of dorsal root ganglia explants and dorsal root ganglia-derived primary cell dissociated cultures. Quantitative data showed that the total neurite length and tortuosity were differently influenced by trophic factors. Nerve growth factor and, indirectly, brain-derived neurotrophic factor stimulate the tortuous growth of sensory fibers and the formation of cell clusters. Neurotrophin-3, however, enhances neurite growth in terms of length and linearity allowing for a more organized and directed axonal elongation towards a peripheral target compared to the other growth factors. These findings could be of considerable importance for any clinical application of neurotrophic factors in peripheral nerve regeneration. Ethical approval was obtained from the Regione Piemonte Animal Ethics Committee ASLTO1(file # 864/2016-PR) on September 14, 2016.
