摘要
采用Ｍｅｒｒｉｆｉｌｄ固相法合成了生长激素释放肽（ＧＨＲＰ）．其氨基酸序列为Ｈｉｓ－Ｄ－Ｔｒｐ－Ａｌａ－Ｔｒｐ－Ｄ－ｐｈｅ－Ｌｙｓ－ＮＨ２．ＨＰＬＣ分析纯度为９７．７％，氨基酸组分分析与理论值吻合．观察了不同剂量对１５、２５及３５日龄小鼠的促生长效应，１０００μｇ／ｋｇ剂量可使小鼠体重较对照组增加１５．２％．当剂量高达１０ｍｇ／ｋｇ时仍安全无毒．
Growth hormone releasing peptide (GHRP) was synthesized by Merrifild's solid phase synthesis of polypeptide. The amino acid sequence of GHRP is H-D-W-A-W-D-F-K-NH2, the purity was 97. 7% obtaining from HPLC, the amino acid composition was found in agreement with its calculated values. When the dosage was 1 000 μg/kg, it enhanced significantly the growth for 15,25 and 35 days mice. The mice's weight had increased by 15. 2% as compared with the contrast when the dosage was 1 000 μg/kg. Although the dosage was 10 000μg/kg, it still had the enhancement effect without toxicity.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
1996年第1期98-101,共4页
Journal of Lanzhou University(Natural Sciences)
基金
甘肃省科技攻关资助
关键词
生长激素释放肽
促生长效应
固相法
合成
solid phase synthesis mouse vitality growth hormone releasing peptide (GHRP) growth enhancement action
