Mature seed-derived callo from two elite Chinese japonica rice (Oryza saliva L.) cultivars Eyi 105 and Ewan 5 were co-transformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectable marker hygromy-cin ...Mature seed-derived callo from two elite Chinese japonica rice (Oryza saliva L.) cultivars Eyi 105 and Ewan 5 were co-transformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectable marker hygromy-cin phosphotransferase gene (hpt), the reporter β-glu-curonidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. 61 independent transgenic rice plants were regenerated from 329 bombarded calli. 79% transgenic plants contained all the three genes, revealed by PCR/Southern blot analysis. Western blot analysis revealed that 36 out of 48 gna-containing transgenic plants expressed GNA (75%) at various levels with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From the R2 generations whose R1 parent plants showing 3:1 Mendelian segregation patterns, we identified five independent homozygous lines containing and expressing all the three transgenes. Insect展开更多
车载钻机具有机动性强、占地面积小等优点,可满足各种复杂路段、井场的转移和摆放需求,已成为地面煤层气抽采孔施工的主流设备。国内车载钻机普遍存在整机质量偏大问题,一方面对汽车安全行驶造成威胁,另一方面不符合我国相关道路法规及...车载钻机具有机动性强、占地面积小等优点,可满足各种复杂路段、井场的转移和摆放需求,已成为地面煤层气抽采孔施工的主流设备。国内车载钻机普遍存在整机质量偏大问题,一方面对汽车安全行驶造成威胁,另一方面不符合我国相关道路法规及机动车产品公告要求。给进机架是车载钻机工作的主要执行部件,约占整机质量的40%,其轻量化研究对于减轻整机质量具有重要意义。从采用高强度材料、结构精细化设计、CAE优化设计3个方面对机架进行了优化,并进行了静态结构分析,最大应力、应变均满足设计要求。在山西省晋城市进行了现场试验,处理孔内事故时最大起拔力达800 k N,给进机架未产生明显变形,验证了其稳定性与安全性。展开更多
Crops are prone to lodging with the decline of stem moisture and the intervention of other factors in the mature harvest period,such as garlic,which is difficult to harvest mechanically.To solve this problem,the plant...Crops are prone to lodging with the decline of stem moisture and the intervention of other factors in the mature harvest period,such as garlic,which is difficult to harvest mechanically.To solve this problem,the plant-correcting reel for harvesting lodging garlic plants,bumped and deformed with plants many times to pull and lift them into a conveyor,is proposed in this study.We analyzed the motion trajectory equation and key influencing factors of the reel and defined the lifting and plant-correcting stages as three processes of contact,stirring and release.For example,the contact deformation model and system energy equations were established in the contact process.Besides,in the stirring process,the garlic plant-correcting conditions were established through the dynamic simulation test analysis of garlic seedling trajectories and the deflection model of garlic stem was constructed.Furthermore,in the release process,the expressions of rubber bars rotation and garlic plant offset bending curvature were constructed and the optimal number and distribution form of bars were determined.Meanwhile,the mechanism and key operating parameters of the auxiliary lifting mechanism of the divider were established.Through the single-factor test,the influence of reel speed,forward speed and reel height on the success feeding rate was analyzed under different bars distribution forms;Through multi-factor experiments,the interaction contour map of various factors was constructed.When reel speed,forward speed and reel height were 3 rad/s,3.5 m/s,and 540 mm,the feeding success rate was 98.73%.The optimization factors were tested and verified,which met the operational requirements of a high feeding success rate and low loss rate of garlic harvest.This study combines laboratory virtual as well as field experiments and analyzes of trajectory of bars,contact deformation and deflection model of garlic plant,and reel rotation and garlic plant offset bending curvature to solve the problem of garlic lodging mechanized harvest and yield reduct展开更多
基金Thiswork was supported by the National "863" High-Tech Program (Grant No. 101-01-01-01) and the Rockefeller Foundation, USA (Grant No. RF97001 #594).
文摘Mature seed-derived callo from two elite Chinese japonica rice (Oryza saliva L.) cultivars Eyi 105 and Ewan 5 were co-transformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectable marker hygromy-cin phosphotransferase gene (hpt), the reporter β-glu-curonidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. 61 independent transgenic rice plants were regenerated from 329 bombarded calli. 79% transgenic plants contained all the three genes, revealed by PCR/Southern blot analysis. Western blot analysis revealed that 36 out of 48 gna-containing transgenic plants expressed GNA (75%) at various levels with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From the R2 generations whose R1 parent plants showing 3:1 Mendelian segregation patterns, we identified five independent homozygous lines containing and expressing all the three transgenes. Insect
文摘车载钻机具有机动性强、占地面积小等优点,可满足各种复杂路段、井场的转移和摆放需求,已成为地面煤层气抽采孔施工的主流设备。国内车载钻机普遍存在整机质量偏大问题,一方面对汽车安全行驶造成威胁,另一方面不符合我国相关道路法规及机动车产品公告要求。给进机架是车载钻机工作的主要执行部件,约占整机质量的40%,其轻量化研究对于减轻整机质量具有重要意义。从采用高强度材料、结构精细化设计、CAE优化设计3个方面对机架进行了优化,并进行了静态结构分析,最大应力、应变均满足设计要求。在山西省晋城市进行了现场试验,处理孔内事故时最大起拔力达800 k N,给进机架未产生明显变形,验证了其稳定性与安全性。
基金China Agriculture Research System of MOF and MARA(CARS-24-D-01)National Key R&D Program of China(Grant No.2023YFD2001200)Shandong Province focuses on supporting the introduction of urgently needed and scarce talent projects in the region.
文摘Crops are prone to lodging with the decline of stem moisture and the intervention of other factors in the mature harvest period,such as garlic,which is difficult to harvest mechanically.To solve this problem,the plant-correcting reel for harvesting lodging garlic plants,bumped and deformed with plants many times to pull and lift them into a conveyor,is proposed in this study.We analyzed the motion trajectory equation and key influencing factors of the reel and defined the lifting and plant-correcting stages as three processes of contact,stirring and release.For example,the contact deformation model and system energy equations were established in the contact process.Besides,in the stirring process,the garlic plant-correcting conditions were established through the dynamic simulation test analysis of garlic seedling trajectories and the deflection model of garlic stem was constructed.Furthermore,in the release process,the expressions of rubber bars rotation and garlic plant offset bending curvature were constructed and the optimal number and distribution form of bars were determined.Meanwhile,the mechanism and key operating parameters of the auxiliary lifting mechanism of the divider were established.Through the single-factor test,the influence of reel speed,forward speed and reel height on the success feeding rate was analyzed under different bars distribution forms;Through multi-factor experiments,the interaction contour map of various factors was constructed.When reel speed,forward speed and reel height were 3 rad/s,3.5 m/s,and 540 mm,the feeding success rate was 98.73%.The optimization factors were tested and verified,which met the operational requirements of a high feeding success rate and low loss rate of garlic harvest.This study combines laboratory virtual as well as field experiments and analyzes of trajectory of bars,contact deformation and deflection model of garlic plant,and reel rotation and garlic plant offset bending curvature to solve the problem of garlic lodging mechanized harvest and yield reduct
