为了解猪脾转移因子(TF)与鸡毒支原体(MG)F弱毒疫苗株联合接种对SPF鸡淋巴细胞比率的影响,以活菌数达109ccu/m L的MG F株0.033 m L/羽与TF 0.02 m L/羽分侧点眼SPF鸡,设为A组;B组以MG F株0.033 m L/羽与生理盐水0.02m L/羽分侧点眼;C组...为了解猪脾转移因子(TF)与鸡毒支原体(MG)F弱毒疫苗株联合接种对SPF鸡淋巴细胞比率的影响,以活菌数达109ccu/m L的MG F株0.033 m L/羽与TF 0.02 m L/羽分侧点眼SPF鸡,设为A组;B组以MG F株0.033 m L/羽与生理盐水0.02m L/羽分侧点眼;C组以生理盐水0.033 m L/羽与TF 0.02 m L/羽分侧点眼;D组以生理盐水0.033 m L/羽和0.02 m L/羽对应分侧点眼,作为对照组。点眼后第7 d采血测定淋巴细胞比率。结果显示,A组(63.9%)、B组(53.8%)和C组(54.6%)的淋巴细胞比率均值分别比D组(41.5%)提高了22.4%、12.3%和13.1%。结果表明,TF与MG F株单独使用均能提高SPF鸡的淋巴细胞比率,且TF与MG F株联合使用后淋巴细胞比率提高值高于单独使用。展开更多
Objective:To study the effects of low concentration of ar senic (As 2O 3) on DNA synthesis and related transcription factor gene E2F1 express ion in keratinocyte. Methods:Human epidermal keratinocyte (cell line HaCa...Objective:To study the effects of low concentration of ar senic (As 2O 3) on DNA synthesis and related transcription factor gene E2F1 express ion in keratinocyte. Methods:Human epidermal keratinocyte (cell line HaCaT) cultured in vitro was used. After treatment with various concentrations of arsenic, DNA s ynthesis and E2F1 expression in HaCaT cells were detected by using 3H-TdR method and RT-PCR.Results:Arsenic caused a modest increase of keratinocyte DNA synth esis when the concentration reached the range within 0.5-16 nmol/L, but the amo unt of incorporated 3H-TdR decreased and returned to baseline level when th e concentration of arsenic increased to over 16 nmol/L. RT-PCR analysis showe d the level of E2F1 mRNA was elevated in HaCaT cells with the increase of DNA sy nthesis. Conclusion:Arsenic of a certain concentration could increase DNA s ynthesis and enhance E2F1 expression in HaCaT cell line, which might be one of t he pathological mechanisms of skin disease related to arsenic.展开更多
文摘为了解猪脾转移因子(TF)与鸡毒支原体(MG)F弱毒疫苗株联合接种对SPF鸡淋巴细胞比率的影响,以活菌数达109ccu/m L的MG F株0.033 m L/羽与TF 0.02 m L/羽分侧点眼SPF鸡,设为A组;B组以MG F株0.033 m L/羽与生理盐水0.02m L/羽分侧点眼;C组以生理盐水0.033 m L/羽与TF 0.02 m L/羽分侧点眼;D组以生理盐水0.033 m L/羽和0.02 m L/羽对应分侧点眼,作为对照组。点眼后第7 d采血测定淋巴细胞比率。结果显示,A组(63.9%)、B组(53.8%)和C组(54.6%)的淋巴细胞比率均值分别比D组(41.5%)提高了22.4%、12.3%和13.1%。结果表明,TF与MG F株单独使用均能提高SPF鸡的淋巴细胞比率,且TF与MG F株联合使用后淋巴细胞比率提高值高于单独使用。
文摘Objective:To study the effects of low concentration of ar senic (As 2O 3) on DNA synthesis and related transcription factor gene E2F1 express ion in keratinocyte. Methods:Human epidermal keratinocyte (cell line HaCaT) cultured in vitro was used. After treatment with various concentrations of arsenic, DNA s ynthesis and E2F1 expression in HaCaT cells were detected by using 3H-TdR method and RT-PCR.Results:Arsenic caused a modest increase of keratinocyte DNA synth esis when the concentration reached the range within 0.5-16 nmol/L, but the amo unt of incorporated 3H-TdR decreased and returned to baseline level when th e concentration of arsenic increased to over 16 nmol/L. RT-PCR analysis showe d the level of E2F1 mRNA was elevated in HaCaT cells with the increase of DNA sy nthesis. Conclusion:Arsenic of a certain concentration could increase DNA s ynthesis and enhance E2F1 expression in HaCaT cell line, which might be one of t he pathological mechanisms of skin disease related to arsenic.
