AIM: To investigate the correlation between ezrin expression and invasive phenotype formation in malignantly transformed esophageal epithelial cells.
METHODS: The experimental cell line employed in the present study w...AIM: To investigate the correlation between ezrin expression and invasive phenotype formation in malignantly transformed esophageal epithelial cells.
METHODS: The experimental cell line employed in the present study was originated form the progressive induction of a human embryonic esophageal epithelial cell line (SHEE)by the E6E7 genes of human papillomavirus (HPV) type 18.The cells at the 35th passage after induction called SHEEIMM were in a state of immortalized phase and used as the control,while that of the 85th passage denominated as SHEEMT represented the status of cells that were malignantly transformed. The expression changes of ezrin and its mRNA in both cell passages were respectively analyzed by RT-PCR and Western blot. Invasive phenotype was assessed in vivo by inoculating these cells into the severe combined immunodeficient (SCID) mice via subcutaneous and intraperitoneal injection, and in vitro by inoculating them on the surface of the amnion membranes, which then was determined by light microscopy and scanning electron microscopy.
RESULTS: Upregulated expression of ezrin protein and its mRNA was observed in SHEEMT compared with that in SHEEIMM cells. The SHEEMT cells inoculated in SCID mice were observed forming tumor masses in both visceral organs and soft tissues in a period of 40 days with a special propensity to invading mesentery and pancreas, but did not exhibit hepatic metastases. Pathologically, these tumor cells harboring larger nucleus, nucleolus and less cytoplasm could infiltrate and destroy adjacent tissues. In the in vitro study,the inoculated SHEEMT cells could grow in cluster on the amniotic epithelial surface and intrude into the amniotic stroma. In contrast, unrestricted growth and invasiveness were not found in SHEEIMM cells in both in vivo and in vitroexperiment.
CONCLUSION: The upregulated ezrin expression is one of the important factors that are possibly associated with the invasive phenotype formation in malignantly transformed esophageal epithelial cells.展开更多
AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METH...AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METHODS: Eighty paraffin-embedded cancer tissue samples were selected from primary colorectal adenocarcinoma. Twenty-eight patients had well-differentiated, 22 had moderately differentiated and 30 had poorly differentiated adenocarcinoma. Forty-five patients and 35 patients had lymph node metastasis. Forty-five patients were of Dukes A to B stage, and 35 were of C to D stage. Another 22 paraffi n-embedded tissue blocks of normal colorectal epithelium (>5 cm away from the edge of the tumor) were selected as the control group. All patients with colorectal cancer were treated surgically and diagnosed histologically, without preoperative chemotherapy or radiotherapy. The immunohistochemistry was used to detect the ezrin expression in paraffin-embedded normal colorectal mucosa tissues and colorectal cancer tissue samples.RESULTS: Ezrin expression in colorectal cancer was significantly higher than in normal colorectal mucosa (75.00% vs 9.09%, P<0.01), and there was a close relationship between ezrin expression and the degree of tumor differentiation, lymph node metastasis and Dukes stage (88.46% vs 50.00%, P<0.01; 94.28% vs 51.11%, P<0.01; 94.28% vs 51.11%, P<0.01).CONCLUSION: Ezrin expression is obviously higher in colorectal cancer tissues than in normal colorectal mucosa tissues, and the high level of ezrin expression is closely related to the colorectal cancer invasion and metastasis process.展开更多
目的探讨益肾通络方对膜性肾病大鼠的肾脏保护作用及可能的机制。方法健康雄性SD大鼠60只随机分为对照组10只和造模组50只,造模组采用尾iv阳离子化牛血清白蛋白(C-BSA)的方法复制膜性肾病大鼠模型。造模成功后再随机分为模型组、盐酸贝...目的探讨益肾通络方对膜性肾病大鼠的肾脏保护作用及可能的机制。方法健康雄性SD大鼠60只随机分为对照组10只和造模组50只,造模组采用尾iv阳离子化牛血清白蛋白(C-BSA)的方法复制膜性肾病大鼠模型。造模成功后再随机分为模型组、盐酸贝那普利组和益肾通络方低、中、高剂量(6.61、13.22、26.44 g/kg)组,各组按照相应剂量ig给药,每天1次,连续给药4周。给药结束后检测大鼠24 h尿蛋白定量(UTP)、血清总胆固醇(TC)、总蛋白(TP)、白蛋白(ALB)、尿素氮(BUN)、肌酐(Scr)水平;免疫荧光检测各组大鼠肾组织Ig G免疫复合物沉积情况,电镜下观察肾小球基底膜及足细胞形态结构;免疫组化及实时荧光定量PCR(q RT-PCR)法检测各组大鼠肾足细胞骨架相关蛋白ezrin、synaptopodin的表达。结果与模型组比较,各治疗组大鼠UTP、TC水平均显著下降(P<0.01),TP、ALB水平均显著上升(P<0.01);益肾通络方中、高剂量组与盐酸贝那普利组疗效相当,效果优于益肾通络方低剂量组;各组大鼠BUN、Scr相比无明显差异。与对照组比较,模型组大鼠肾足细胞骨架相关蛋白ezrin、synaptopodin m RNA表达显著减少(P<0.01);与模型组比较,各治疗组大鼠肾足细胞相关蛋白ezrin、synaptopodin m RNA表达均有不同程度增加(P<0.01);益肾通络方中、高剂量组大鼠肾组织ezrin、synaptopodin m RNA表达量与盐酸贝那普利组相当,均高于益肾通络方低剂量组。结论益肾通络方对膜性肾病大鼠具有治疗作用,其机制可能与防止足细胞骨架相关蛋白ezrin、synaptopodin降解、维持足细胞骨架及足突结构完整性相关。展开更多
基金the National Natural Science Foundation of China (No.39830380,39900069)Research and Development Foundation of Shantou University(L00012)the Chinese National Human Genome Center,Beijing
文摘AIM: To investigate the correlation between ezrin expression and invasive phenotype formation in malignantly transformed esophageal epithelial cells.
METHODS: The experimental cell line employed in the present study was originated form the progressive induction of a human embryonic esophageal epithelial cell line (SHEE)by the E6E7 genes of human papillomavirus (HPV) type 18.The cells at the 35th passage after induction called SHEEIMM were in a state of immortalized phase and used as the control,while that of the 85th passage denominated as SHEEMT represented the status of cells that were malignantly transformed. The expression changes of ezrin and its mRNA in both cell passages were respectively analyzed by RT-PCR and Western blot. Invasive phenotype was assessed in vivo by inoculating these cells into the severe combined immunodeficient (SCID) mice via subcutaneous and intraperitoneal injection, and in vitro by inoculating them on the surface of the amnion membranes, which then was determined by light microscopy and scanning electron microscopy.
RESULTS: Upregulated expression of ezrin protein and its mRNA was observed in SHEEMT compared with that in SHEEIMM cells. The SHEEMT cells inoculated in SCID mice were observed forming tumor masses in both visceral organs and soft tissues in a period of 40 days with a special propensity to invading mesentery and pancreas, but did not exhibit hepatic metastases. Pathologically, these tumor cells harboring larger nucleus, nucleolus and less cytoplasm could infiltrate and destroy adjacent tissues. In the in vitro study,the inoculated SHEEMT cells could grow in cluster on the amniotic epithelial surface and intrude into the amniotic stroma. In contrast, unrestricted growth and invasiveness were not found in SHEEIMM cells in both in vivo and in vitroexperiment.
CONCLUSION: The upregulated ezrin expression is one of the important factors that are possibly associated with the invasive phenotype formation in malignantly transformed esophageal epithelial cells.
基金Supported by Natural Science Foundation of Shanghai,No.04ZB14072
文摘AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METHODS: Eighty paraffin-embedded cancer tissue samples were selected from primary colorectal adenocarcinoma. Twenty-eight patients had well-differentiated, 22 had moderately differentiated and 30 had poorly differentiated adenocarcinoma. Forty-five patients and 35 patients had lymph node metastasis. Forty-five patients were of Dukes A to B stage, and 35 were of C to D stage. Another 22 paraffi n-embedded tissue blocks of normal colorectal epithelium (>5 cm away from the edge of the tumor) were selected as the control group. All patients with colorectal cancer were treated surgically and diagnosed histologically, without preoperative chemotherapy or radiotherapy. The immunohistochemistry was used to detect the ezrin expression in paraffin-embedded normal colorectal mucosa tissues and colorectal cancer tissue samples.RESULTS: Ezrin expression in colorectal cancer was significantly higher than in normal colorectal mucosa (75.00% vs 9.09%, P<0.01), and there was a close relationship between ezrin expression and the degree of tumor differentiation, lymph node metastasis and Dukes stage (88.46% vs 50.00%, P<0.01; 94.28% vs 51.11%, P<0.01; 94.28% vs 51.11%, P<0.01).CONCLUSION: Ezrin expression is obviously higher in colorectal cancer tissues than in normal colorectal mucosa tissues, and the high level of ezrin expression is closely related to the colorectal cancer invasion and metastasis process.
文摘目的探讨益肾通络方对膜性肾病大鼠的肾脏保护作用及可能的机制。方法健康雄性SD大鼠60只随机分为对照组10只和造模组50只,造模组采用尾iv阳离子化牛血清白蛋白(C-BSA)的方法复制膜性肾病大鼠模型。造模成功后再随机分为模型组、盐酸贝那普利组和益肾通络方低、中、高剂量(6.61、13.22、26.44 g/kg)组,各组按照相应剂量ig给药,每天1次,连续给药4周。给药结束后检测大鼠24 h尿蛋白定量(UTP)、血清总胆固醇(TC)、总蛋白(TP)、白蛋白(ALB)、尿素氮(BUN)、肌酐(Scr)水平;免疫荧光检测各组大鼠肾组织Ig G免疫复合物沉积情况,电镜下观察肾小球基底膜及足细胞形态结构;免疫组化及实时荧光定量PCR(q RT-PCR)法检测各组大鼠肾足细胞骨架相关蛋白ezrin、synaptopodin的表达。结果与模型组比较,各治疗组大鼠UTP、TC水平均显著下降(P<0.01),TP、ALB水平均显著上升(P<0.01);益肾通络方中、高剂量组与盐酸贝那普利组疗效相当,效果优于益肾通络方低剂量组;各组大鼠BUN、Scr相比无明显差异。与对照组比较,模型组大鼠肾足细胞骨架相关蛋白ezrin、synaptopodin m RNA表达显著减少(P<0.01);与模型组比较,各治疗组大鼠肾足细胞相关蛋白ezrin、synaptopodin m RNA表达均有不同程度增加(P<0.01);益肾通络方中、高剂量组大鼠肾组织ezrin、synaptopodin m RNA表达量与盐酸贝那普利组相当,均高于益肾通络方低剂量组。结论益肾通络方对膜性肾病大鼠具有治疗作用,其机制可能与防止足细胞骨架相关蛋白ezrin、synaptopodin降解、维持足细胞骨架及足突结构完整性相关。
