为探讨芍药甘草汤配伍机制与适宜的配伍比例,采用大鼠外翻肠囊模型、在体单向肠灌流模型,从肠吸收角度研究芍药甘草汤SG11(白芍-炙甘草1∶1)、SG31(白芍-炙甘草3∶1)、白芍水煎液S及炙甘草水煎液(G)中主要指标成分在大鼠离体、在体肠吸...为探讨芍药甘草汤配伍机制与适宜的配伍比例,采用大鼠外翻肠囊模型、在体单向肠灌流模型,从肠吸收角度研究芍药甘草汤SG11(白芍-炙甘草1∶1)、SG31(白芍-炙甘草3∶1)、白芍水煎液S及炙甘草水煎液(G)中主要指标成分在大鼠离体、在体肠吸收情况,并进行比较。以HPLC测定上述供试品以及肠囊吸收样品、肠灌流样品中芍药苷(paeoniflorin,PF)、甘草苷(liquiritin,LQ)、甘草酸单铵盐(glycyrrhizin single ammonium salt,GL)的浓度,并计算肠吸收量及吸收参数。发现在外翻肠囊模型中,3种成分在十二指肠、空肠和回肠均有吸收,最佳吸收部位均为空肠,且SG11中相应成分的吸收率均显著高于单煎(P<0.05),与SG31比较无显著性差异。在单向在体肠灌流模型中,SG11中相应成分的吸收速率常数Ka、表观吸收系数Papp和吸收率均显著高于各单煎液,SG11中PF,GL的各参数均显著高于SG31,LQ,各参数无差异。说明白芍炙甘草配伍有助于PF,LQ和GL的肠吸收,SG11较SG31各指标成分的吸收好。展开更多
目的研究吴茱萸汤中9种成分的量对其自身吸收进入外翻肠囊成分量的影响,以指导吴茱萸汤改善偏头痛模型鼠指标的样品的调配。方法采用大鼠外翻肠囊模型获取10种吴茱萸汤吸收样品,以HPLC-DAD对吴茱萸汤及外翻肠囊吸收样品中9种成分进行定...目的研究吴茱萸汤中9种成分的量对其自身吸收进入外翻肠囊成分量的影响,以指导吴茱萸汤改善偏头痛模型鼠指标的样品的调配。方法采用大鼠外翻肠囊模型获取10种吴茱萸汤吸收样品,以HPLC-DAD对吴茱萸汤及外翻肠囊吸收样品中9种成分进行定量、半定量,得到各成分在吴茱萸汤中的量以及在肠囊中的累积吸收量。用偏最小二乘回归法(partial least squares regression,PLSR)对吴茱萸汤中各成分的量及总体吸收量进行相关分析。结果在实验质量浓度范围内,柠檬黄素-3-O-β-D-葡萄糖苷、人参皂苷Rg1、6-姜辣素、吴茱萸苦素在吴茱萸汤中质量浓度越高,吸收入肠囊的总量越多;而人参皂苷Re、人参皂苷Rb1、柠檬苦素、吴茱萸碱、吴茱萸次碱则相反。成分之间对吸收的相互影响关系十分复杂。结论在根据吸收谱-效相关分析结果调配吴茱萸汤样品时,应考虑成分吸收的相互影响关系以达到较佳疗效。展开更多
Cortex Moutan (Paeonia suffruticosa Andr.) is a common traditional Chinese medicine and has been widely used in clinic for 2000 years in China. As sources for this crude drug are always mixed with other species, many ...Cortex Moutan (Paeonia suffruticosa Andr.) is a common traditional Chinese medicine and has been widely used in clinic for 2000 years in China. As sources for this crude drug are always mixed with other species, many cultivars on herbal market may lead to quality instability. Multi-component quantitative analysis is an efficient method to reflect chemical profiles of herb medicine and is always taken as the main method for quality evaluation. So, the aim of this work is to develop analytical method to quantify paeonol, paeoniflorin, gallic acid, oxypaeoniflorin, benzoylpaeoniflorin and paeonolide in Cortex Moutan (CM) to evaluate the chemical qualities of CM from different species or cultivars. Besides, we also study the intestinal absorption characteristics of paeonol and paeoniflorin for further pharmacological evaluation. In the present study, all of the standard markers were performed on an Ecosil C18 (250 mm × 4.6 mm, 5 μM, Lubex Co., Guangzhou, China) with linear gradient elution of 0.2% formic acid water and acetonitrile. The proposed method was applied to analyze 50 batches of samples with acceptable linearity (R2, 0.9995 - 0.9999), precisions (RSD, 0.47% - 2.08%), repeatability (RSD, 039% - 2.63%), stability (RSD, 0.52% - 2.45%), and recovery (RSD, 0.72% - 3.03%) of the six compounds. Furthermore, the Hierarchical Cluster Analysis was applied to classify the 50 samples based on contents of the six compound markers. The results obtained from multi-component quantification of CM clearly indicated that CM originated from P. suffruticosa and P. ostii presented different chemical properties, and that samples from the two materials could be gathered into one branch, respectively, while CM sourced from cultivars of P. suffruticosa showed great variety on chemical quality. The results from Hierarchical Cluster Analysis implied that the established method could be used as a powerful tool for the quality evaluation of CM. The intestinal absorption study indicated that the intestinal absorption activities for pae展开更多
文摘为探讨芍药甘草汤配伍机制与适宜的配伍比例,采用大鼠外翻肠囊模型、在体单向肠灌流模型,从肠吸收角度研究芍药甘草汤SG11(白芍-炙甘草1∶1)、SG31(白芍-炙甘草3∶1)、白芍水煎液S及炙甘草水煎液(G)中主要指标成分在大鼠离体、在体肠吸收情况,并进行比较。以HPLC测定上述供试品以及肠囊吸收样品、肠灌流样品中芍药苷(paeoniflorin,PF)、甘草苷(liquiritin,LQ)、甘草酸单铵盐(glycyrrhizin single ammonium salt,GL)的浓度,并计算肠吸收量及吸收参数。发现在外翻肠囊模型中,3种成分在十二指肠、空肠和回肠均有吸收,最佳吸收部位均为空肠,且SG11中相应成分的吸收率均显著高于单煎(P<0.05),与SG31比较无显著性差异。在单向在体肠灌流模型中,SG11中相应成分的吸收速率常数Ka、表观吸收系数Papp和吸收率均显著高于各单煎液,SG11中PF,GL的各参数均显著高于SG31,LQ,各参数无差异。说明白芍炙甘草配伍有助于PF,LQ和GL的肠吸收,SG11较SG31各指标成分的吸收好。
文摘目的研究吴茱萸汤中9种成分的量对其自身吸收进入外翻肠囊成分量的影响,以指导吴茱萸汤改善偏头痛模型鼠指标的样品的调配。方法采用大鼠外翻肠囊模型获取10种吴茱萸汤吸收样品,以HPLC-DAD对吴茱萸汤及外翻肠囊吸收样品中9种成分进行定量、半定量,得到各成分在吴茱萸汤中的量以及在肠囊中的累积吸收量。用偏最小二乘回归法(partial least squares regression,PLSR)对吴茱萸汤中各成分的量及总体吸收量进行相关分析。结果在实验质量浓度范围内,柠檬黄素-3-O-β-D-葡萄糖苷、人参皂苷Rg1、6-姜辣素、吴茱萸苦素在吴茱萸汤中质量浓度越高,吸收入肠囊的总量越多;而人参皂苷Re、人参皂苷Rb1、柠檬苦素、吴茱萸碱、吴茱萸次碱则相反。成分之间对吸收的相互影响关系十分复杂。结论在根据吸收谱-效相关分析结果调配吴茱萸汤样品时,应考虑成分吸收的相互影响关系以达到较佳疗效。
文摘Cortex Moutan (Paeonia suffruticosa Andr.) is a common traditional Chinese medicine and has been widely used in clinic for 2000 years in China. As sources for this crude drug are always mixed with other species, many cultivars on herbal market may lead to quality instability. Multi-component quantitative analysis is an efficient method to reflect chemical profiles of herb medicine and is always taken as the main method for quality evaluation. So, the aim of this work is to develop analytical method to quantify paeonol, paeoniflorin, gallic acid, oxypaeoniflorin, benzoylpaeoniflorin and paeonolide in Cortex Moutan (CM) to evaluate the chemical qualities of CM from different species or cultivars. Besides, we also study the intestinal absorption characteristics of paeonol and paeoniflorin for further pharmacological evaluation. In the present study, all of the standard markers were performed on an Ecosil C18 (250 mm × 4.6 mm, 5 μM, Lubex Co., Guangzhou, China) with linear gradient elution of 0.2% formic acid water and acetonitrile. The proposed method was applied to analyze 50 batches of samples with acceptable linearity (R2, 0.9995 - 0.9999), precisions (RSD, 0.47% - 2.08%), repeatability (RSD, 039% - 2.63%), stability (RSD, 0.52% - 2.45%), and recovery (RSD, 0.72% - 3.03%) of the six compounds. Furthermore, the Hierarchical Cluster Analysis was applied to classify the 50 samples based on contents of the six compound markers. The results obtained from multi-component quantification of CM clearly indicated that CM originated from P. suffruticosa and P. ostii presented different chemical properties, and that samples from the two materials could be gathered into one branch, respectively, while CM sourced from cultivars of P. suffruticosa showed great variety on chemical quality. The results from Hierarchical Cluster Analysis implied that the established method could be used as a powerful tool for the quality evaluation of CM. The intestinal absorption study indicated that the intestinal absorption activities for pae
