The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined. After administration of 10-80 μmol/L dracorhodin perchlorate for 12-48 h, cell viabili...The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined. After administration of 10-80 μmol/L dracorhodin perchlorate for 12-48 h, cell viability of PC-3 cells was measured by MTT colorimetry. Cell proliferation ability was detected by colony formation assay. Cellular apoptosis was inspected by acridine orange-ethidium bromide fluorescent staining, Hoechst 33258 fluorescent staining, and flow cytometry （FCM） with annexin Ⅴ-FITC/propidium iodide dual staining. The results showed that dracorhodin perchlorate inhibited the growth of PC-3 in a dose- and time-dependent manner. IC50 of dracorhodin perchlorate on PC-3 cells at 24 h was 40.18 μmol/L. Cell clone formation rate was decreased by 86% after treatment with 20 μmol/L of dracorhodin perchlorate. Some cells presented the characteristic apoptotic changes. The cellular apoptotic rates induced by 10-40 μmol/L dracorhodin perchlorate for 24 h were 8.43% to 47.71% respectively. It was concluded that dracorhodin perchlorate significantly inhibited the growth of PC-3 cells by suppressing proliferation and inducing apoptosis of the cells.展开更多
目的采用HPLC法测定展筋活血散中血竭素的含量。方法采用HPLC法。色谱柱:CAPCELL PAK C18MGⅡ色谱柱(250mm×4.6mm,5μm);流动相:乙腈-0.05mol·L-1磷酸二氢钠溶液(50∶50);流速:1.0mL·min-1;检测波长:440nm;柱温:40℃。...目的采用HPLC法测定展筋活血散中血竭素的含量。方法采用HPLC法。色谱柱:CAPCELL PAK C18MGⅡ色谱柱(250mm×4.6mm,5μm);流动相:乙腈-0.05mol·L-1磷酸二氢钠溶液(50∶50);流速:1.0mL·min-1;检测波长:440nm;柱温:40℃。结果血竭素在0.027 7～2.210 9μg范围内线性关系良好(r=0.999 9),平均回收率为98.8%(RSD为0.9%,n=6)。结论该方法简便灵敏,精密度好,结果准确可靠。展开更多
基金supported by a grant from the Key Project of Science and Technology of Wuhan (No. 201060938361-08)
文摘The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined. After administration of 10-80 μmol/L dracorhodin perchlorate for 12-48 h, cell viability of PC-3 cells was measured by MTT colorimetry. Cell proliferation ability was detected by colony formation assay. Cellular apoptosis was inspected by acridine orange-ethidium bromide fluorescent staining, Hoechst 33258 fluorescent staining, and flow cytometry （FCM） with annexin Ⅴ-FITC/propidium iodide dual staining. The results showed that dracorhodin perchlorate inhibited the growth of PC-3 in a dose- and time-dependent manner. IC50 of dracorhodin perchlorate on PC-3 cells at 24 h was 40.18 μmol/L. Cell clone formation rate was decreased by 86% after treatment with 20 μmol/L of dracorhodin perchlorate. Some cells presented the characteristic apoptotic changes. The cellular apoptotic rates induced by 10-40 μmol/L dracorhodin perchlorate for 24 h were 8.43% to 47.71% respectively. It was concluded that dracorhodin perchlorate significantly inhibited the growth of PC-3 cells by suppressing proliferation and inducing apoptosis of the cells.
