优化建立鲤鱼(cyprinus carpio,CC)嗅觉端脑(嗅脑)全蛋白提取技术。联用低渗透裂解和液氮冻溶法破碎鲤鱼嗅脑组织(rhinencephalon tissue of cyprinus carpio,CCRT)、低速离心提取CCRT全蛋白,并采用双向凝胶电泳(2D-PAGE)技术进行有效...优化建立鲤鱼(cyprinus carpio,CC)嗅觉端脑(嗅脑)全蛋白提取技术。联用低渗透裂解和液氮冻溶法破碎鲤鱼嗅脑组织(rhinencephalon tissue of cyprinus carpio,CCRT)、低速离心提取CCRT全蛋白,并采用双向凝胶电泳(2D-PAGE)技术进行有效分离。经分析与统计,每张CCRT的2D-PAGE图谱中的蛋白质斑点数目约为1200个。分别分离CCRT的脂溶性和水溶性全蛋白,并获得高分辨率的2D-PAGE图谱。选用差异蛋白质组学技术筛选经10%冰醋酸创伤后的CC,其端脑组织所表达出的6种应激蛋白质,并用肽质量指纹谱(peptide mass fingerprinting,PMF)和数据库检索技术给予鉴定。其中3种蛋白质为70S热休克蛋白、β微管蛋白和DNA链接酶IV,有望作为研究大脑急性创伤后的应激修复途径和机理的指示蛋白质。展开更多
Finding exact solutions for Riemann–Liouville(RL)fractional equations is very difficult.We propose a general method of separation of variables to study the problem.We obtain several general results and,as application...Finding exact solutions for Riemann–Liouville(RL)fractional equations is very difficult.We propose a general method of separation of variables to study the problem.We obtain several general results and,as applications,we give nontrivial exact solutions for some typical RL fractional equations such as the fractional Kadomtsev–Petviashvili equation and the fractional Langmuir chain equation.In particular,we obtain non-power functions solutions for a kind of RL time-fractional reaction–diffusion equation.In addition,we find that the separation of variables method is more suited to deal with high-dimensional nonlinear RL fractional equations because we have more freedom to choose undetermined functions.展开更多
In this paper, the Schr?dinger equation is solved by Modified separation of variables (MSV) method suggested by Pishkoo and Darus. Using this method, Meijer’s G-function solutions are derived in cylindrical coordinat...In this paper, the Schr?dinger equation is solved by Modified separation of variables (MSV) method suggested by Pishkoo and Darus. Using this method, Meijer’s G-function solutions are derived in cylindrical coordinate system for quantum particle in cylindrical can. All elementary functions and most of the special functions which are the solution of extensive problems in physics and engineering are special cases of Meijer’s G-functions.展开更多
文摘优化建立鲤鱼(cyprinus carpio,CC)嗅觉端脑(嗅脑)全蛋白提取技术。联用低渗透裂解和液氮冻溶法破碎鲤鱼嗅脑组织(rhinencephalon tissue of cyprinus carpio,CCRT)、低速离心提取CCRT全蛋白,并采用双向凝胶电泳(2D-PAGE)技术进行有效分离。经分析与统计,每张CCRT的2D-PAGE图谱中的蛋白质斑点数目约为1200个。分别分离CCRT的脂溶性和水溶性全蛋白,并获得高分辨率的2D-PAGE图谱。选用差异蛋白质组学技术筛选经10%冰醋酸创伤后的CC,其端脑组织所表达出的6种应激蛋白质,并用肽质量指纹谱(peptide mass fingerprinting,PMF)和数据库检索技术给予鉴定。其中3种蛋白质为70S热休克蛋白、β微管蛋白和DNA链接酶IV,有望作为研究大脑急性创伤后的应激修复途径和机理的指示蛋白质。
文摘Finding exact solutions for Riemann–Liouville(RL)fractional equations is very difficult.We propose a general method of separation of variables to study the problem.We obtain several general results and,as applications,we give nontrivial exact solutions for some typical RL fractional equations such as the fractional Kadomtsev–Petviashvili equation and the fractional Langmuir chain equation.In particular,we obtain non-power functions solutions for a kind of RL time-fractional reaction–diffusion equation.In addition,we find that the separation of variables method is more suited to deal with high-dimensional nonlinear RL fractional equations because we have more freedom to choose undetermined functions.
文摘In this paper, the Schr?dinger equation is solved by Modified separation of variables (MSV) method suggested by Pishkoo and Darus. Using this method, Meijer’s G-function solutions are derived in cylindrical coordinate system for quantum particle in cylindrical can. All elementary functions and most of the special functions which are the solution of extensive problems in physics and engineering are special cases of Meijer’s G-functions.
