目的研究低频复合生理频率慢性电刺激后肺气肿兔膈肌肌球蛋白重链(myosin heavy chain,MHC)亚型及其力学模式的适应性变化。方法采用木瓜蛋白酶雾化吸入法建立肺气肿模型:测定正常对照组、肺气肿组和肺气肿各慢性电刺激(chronic electri...目的研究低频复合生理频率慢性电刺激后肺气肿兔膈肌肌球蛋白重链(myosin heavy chain,MHC)亚型及其力学模式的适应性变化。方法采用木瓜蛋白酶雾化吸入法建立肺气肿模型:测定正常对照组、肺气肿组和肺气肿各慢性电刺激(chronic electrical stimulation,CES)组膈肌MHC亚型及膈肌颤搐收缩张力(Pt)、峰值张力时间(TPT)、1/2松弛时间(1/2Rt)、强直颤搐收缩张力(Po)、疲劳指数(FI)和疲劳恢复指数(FRI)的适应性变化。结果①40Hz、10Hz、(10+40)Hz慢性电刺激后肺气肿兔膈肌MHC的相对含量较刺激前都明显增加(P<0·05)。10Hz组MHC-I型比例明显增加(P<0·01),(10+40)Hz组MHC-I、MHC-IIa型比例增加(P<0·05)。②肺气肿组Pt、Po均较正常对照组明显减低(P<0·01),TPT、1/2Rt延长(P<0·01),FI、FRI增加(P<0·01);(10+40)Hz组和40Hz组与肺气肿组比较Pt、Po均明显增加(P<0·01),TPT、1/2Rt均明显缩短(P<0·01),FI和FRI下降(P<0·01);(10+40)Hz组与40Hz组比较,Pt、Po、TPT、1/2Rt无显著性差异(P>0·05),FI和FRI降低(P<0·01)。结论不同频率的CES可导致膈肌MHC亚型及肌条力学产生不同的适应性变化,低频复合生理频率电刺激可显著提高肺气肿兔膈肌收缩力,增强膈肌抗疲劳能力,可能是体外膈肌起搏对COPD患者膈肌康复治疗更佳的频率模式之一。展开更多
In this work, mechanisms of influence of protein sensibility of an organism on contractile function of the isolated skeletal muscles of the mouse—“fast”—musculus extensor digitorum longus, “mixed”—musculus diap...In this work, mechanisms of influence of protein sensibility of an organism on contractile function of the isolated skeletal muscles of the mouse—“fast”—musculus extensor digitorum longus, “mixed”—musculus diaphragma and “slow”— musculus soleus are investigated. It is shown that at a protein sensitization all “fast”, “mixed” and “slow” skeletal muscles change the contractile properties. The vector of these changes for muscles with a various phenotypes carries opposite character. Force of the reduction caused carbacholine at a “slow” and “mixed” muscles increase, at “fast”—decreases. A vector of change of force of reduction on carbacholine at protein sensitization at these skeletal muscles correlates with changes of non-quantum secretion acetylcholine in a zone of a trailer plate. Opposite changes of functional properties of “fast” and “mixed” muscles and “slow” muscles of a shin of the mouse at protein sensitization are caused by dynamics cholinoceptive processes of excitation of membrane muscular fibers. It comes out with the assumption, that change of the contraction functions of skeletal muscles at protein sensitization is caused by changes of cholinoceptive processes of excitation of a membrane of muscular fibers, and other changes in system of electro-mechanical interface.展开更多
文摘目的研究低频复合生理频率慢性电刺激后肺气肿兔膈肌肌球蛋白重链(myosin heavy chain,MHC)亚型及其力学模式的适应性变化。方法采用木瓜蛋白酶雾化吸入法建立肺气肿模型:测定正常对照组、肺气肿组和肺气肿各慢性电刺激(chronic electrical stimulation,CES)组膈肌MHC亚型及膈肌颤搐收缩张力(Pt)、峰值张力时间(TPT)、1/2松弛时间(1/2Rt)、强直颤搐收缩张力(Po)、疲劳指数(FI)和疲劳恢复指数(FRI)的适应性变化。结果①40Hz、10Hz、(10+40)Hz慢性电刺激后肺气肿兔膈肌MHC的相对含量较刺激前都明显增加(P<0·05)。10Hz组MHC-I型比例明显增加(P<0·01),(10+40)Hz组MHC-I、MHC-IIa型比例增加(P<0·05)。②肺气肿组Pt、Po均较正常对照组明显减低(P<0·01),TPT、1/2Rt延长(P<0·01),FI、FRI增加(P<0·01);(10+40)Hz组和40Hz组与肺气肿组比较Pt、Po均明显增加(P<0·01),TPT、1/2Rt均明显缩短(P<0·01),FI和FRI下降(P<0·01);(10+40)Hz组与40Hz组比较,Pt、Po、TPT、1/2Rt无显著性差异(P>0·05),FI和FRI降低(P<0·01)。结论不同频率的CES可导致膈肌MHC亚型及肌条力学产生不同的适应性变化,低频复合生理频率电刺激可显著提高肺气肿兔膈肌收缩力,增强膈肌抗疲劳能力,可能是体外膈肌起搏对COPD患者膈肌康复治疗更佳的频率模式之一。
文摘In this work, mechanisms of influence of protein sensibility of an organism on contractile function of the isolated skeletal muscles of the mouse—“fast”—musculus extensor digitorum longus, “mixed”—musculus diaphragma and “slow”— musculus soleus are investigated. It is shown that at a protein sensitization all “fast”, “mixed” and “slow” skeletal muscles change the contractile properties. The vector of these changes for muscles with a various phenotypes carries opposite character. Force of the reduction caused carbacholine at a “slow” and “mixed” muscles increase, at “fast”—decreases. A vector of change of force of reduction on carbacholine at protein sensitization at these skeletal muscles correlates with changes of non-quantum secretion acetylcholine in a zone of a trailer plate. Opposite changes of functional properties of “fast” and “mixed” muscles and “slow” muscles of a shin of the mouse at protein sensitization are caused by dynamics cholinoceptive processes of excitation of membrane muscular fibers. It comes out with the assumption, that change of the contraction functions of skeletal muscles at protein sensitization is caused by changes of cholinoceptive processes of excitation of a membrane of muscular fibers, and other changes in system of electro-mechanical interface.
