Iodine, Dragendorff's reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (T...Iodine, Dragendorff's reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (TLC). Cyclotides could be distinguished from the others by comparing the TLC col-oring plots with G-250 and ninhydrin reagents. With this method, cyclotides were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.展开更多
研究使用响应面法优化Fenton试剂脱色考马斯亮蓝G-250废液.探究pH、H2O2和FeSO4溶液浓度三个单因素对G-250废液褪色率的影响.在单因素实验的基础之上,响应面法优化G-250废液脱色条件.结果表明,Fenton试剂脱色考马斯亮蓝废液G-250的最佳...研究使用响应面法优化Fenton试剂脱色考马斯亮蓝G-250废液.探究pH、H2O2和FeSO4溶液浓度三个单因素对G-250废液褪色率的影响.在单因素实验的基础之上,响应面法优化G-250废液脱色条件.结果表明,Fenton试剂脱色考马斯亮蓝废液G-250的最佳优化条件为:20 mL G-250废液体系中,H2O2投加量为60μL,FeSO4投加浓度为1.5 g/L,pH值为4,该条件下G-250废液褪色率为95.93%.光谱扫描结果表明褪色的废液中含有大量具有苯环结构的分子.实验验证结果为实验室考马斯亮蓝G-250废液的脱色提供了实验依据.展开更多
基金This work was supported by Natural Science Key Research Project of Anhui Provincial EducationDepartment(KJ2007A131ZC)Professor/Doctor Foundation of Suzhou College(2006jb05)
文摘利用光谱探针技术研究在酸性溶液中考马斯亮蓝G-250(Coomassie brilliant blue G-250,CBBG)与牛血清白蛋白(bovine serum albumin,BSA)相互作用机理,考察了不同实验条件对CBBG-BSA复合物吸收光谱的影响。实验结果表明:CBBG与BSA相互作用产生光谱蓝移主要是由CBBG与BSA间的疏水相互作用引起,而静电作用则是形成CBBG-BSA蓝色复合物的必要条件。同时,CBBG聚集体的聚集程度是影响CBBG-BSA蓝色复合物形成的重要因素。
基金the National Natural Science Foundation of China (Grant No. 30725048)Foundation of the West Light Program of Chinese Academy of Sciences
文摘Iodine, Dragendorff's reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (TLC). Cyclotides could be distinguished from the others by comparing the TLC col-oring plots with G-250 and ninhydrin reagents. With this method, cyclotides were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.
文摘研究使用响应面法优化Fenton试剂脱色考马斯亮蓝G-250废液.探究pH、H2O2和FeSO4溶液浓度三个单因素对G-250废液褪色率的影响.在单因素实验的基础之上,响应面法优化G-250废液脱色条件.结果表明,Fenton试剂脱色考马斯亮蓝废液G-250的最佳优化条件为:20 mL G-250废液体系中,H2O2投加量为60μL,FeSO4投加浓度为1.5 g/L,pH值为4,该条件下G-250废液褪色率为95.93%.光谱扫描结果表明褪色的废液中含有大量具有苯环结构的分子.实验验证结果为实验室考马斯亮蓝G-250废液的脱色提供了实验依据.
