The astrocyte is a critical regulator of neuronal survival after ischemic brain injury. Electroacupuncture may be an effective therapy for cerebral ischemia, as electroacupuncture frequency can affect the structural i...The astrocyte is a critical regulator of neuronal survival after ischemic brain injury. Electroacupuncture may be an effective therapy for cerebral ischemia, as electroacupuncture frequency can affect the structural integdty of astrocytes. In this study, a rat model of middle cerebral artery occlusion established using the modified thread embolism method was treated with electroacupuncture of the bilateral Quchi （Llll） and Zusanfi （ST36） at 15, 30, and 100 Hz frequencies. Behavioral testing, immunohistochemistry and electron microscopy were used to explore the effect of these electroacupuncture frequencies used on maintaining the structural integrity of ischemic brain tissue. Compared with the model and 100 Hz electroacupuncture groups the 15 and 30 Hz electroacupuncture groups displayed decreased neurological deficit scores, as evaluated by the ＂Longa＂ method, significantly increased glial fibrillary acidic protein expression, and alleviated ultrastructural damage of astrocytes at the edge of the infarct. Our experimental findings indicate that 15 and 30 Hz electroacupuncture intervention can favorably maintain the structural integrity of astrocytes and play a protective role in cerebral ischemic injury. Astrocyte structural integrity may be the mechanism underlying acupuncture production of ischemic tolerance展开更多
The involvement of apoptosis in mitochondrial toxin 3 nitropropionic acid (3 NPA) induced ischemic tolerance to transient focal cerebral ischemia in rats and the mechanism was investigated. 3 NPA at a dose of 20 m...The involvement of apoptosis in mitochondrial toxin 3 nitropropionic acid (3 NPA) induced ischemic tolerance to transient focal cerebral ischemia in rats and the mechanism was investigated. 3 NPA at a dose of 20 mg/kg or vehicle control was intraperitoneally into the rats. Three days later, rats were exposed to 2 h of middle cerebral artery occlusion followed by 24 h of reperfusion. Infarct volumes were assessed by 2,3,5 triphenyltetrazolinm chloride (TTC) staining 24 h after reperfusion. Neural cell apoptosis in cerebral ischemic penumbra was detected by terminal deoxynucleotidyl transferase mediated dUTP biotin in situ nick end labeling (TUNEL) and flow cytometry methods (FCM). The results showed that as compared to the vehicle treated group, pretreatment with 3 NPA could reduce the infarct volume by 23.3 % and decrease the number of TUNEL positive neural cells and apoptotic percentage by 47 % ( P< 0.05) and 44.9 % ( P< 0 01), respectively. It was concluded that the development of 3 NPA induced ischemic tolerance in brain might be related to the decreases in neural cell apoptosis.展开更多
目的 观察局灶性脑缺血预处理对大鼠脑 15 -脂氧化酶 (15 - L O)表达及其 m RNA(15 - L O m RNA)转录的影响 ,探讨两者在诱导脑缺血耐受中的意义。方法 线栓法制备大鼠局灶性脑预缺血 -缺血再灌注模型 (PC-MCAO)及局灶性单纯脑缺血再...目的 观察局灶性脑缺血预处理对大鼠脑 15 -脂氧化酶 (15 - L O)表达及其 m RNA(15 - L O m RNA)转录的影响 ,探讨两者在诱导脑缺血耐受中的意义。方法 线栓法制备大鼠局灶性脑预缺血 -缺血再灌注模型 (PC-MCAO)及局灶性单纯脑缺血再灌注模型 (MCAO) ,各组于不同时程用免疫组化法检测 15 - L O的表达、原位杂交法检测 15 - L O m RNA的表达。结果 PC- MCAO组较对应时程的 MCAO组 15 - L O m RNA及其蛋白 15 - L O的表达强度显著降低 (P<0 .0 5 )。结论 脑预缺血处理可致 15 - L O及其基因表达下调 ,这可能是局灶性脑缺血预处理诱导脑缺血耐受的机制之一。展开更多
基金supported by a grant from the Guangxi Administration of Traditional Chinese Medicine,No.GZZC0962
文摘The astrocyte is a critical regulator of neuronal survival after ischemic brain injury. Electroacupuncture may be an effective therapy for cerebral ischemia, as electroacupuncture frequency can affect the structural integdty of astrocytes. In this study, a rat model of middle cerebral artery occlusion established using the modified thread embolism method was treated with electroacupuncture of the bilateral Quchi （Llll） and Zusanfi （ST36） at 15, 30, and 100 Hz frequencies. Behavioral testing, immunohistochemistry and electron microscopy were used to explore the effect of these electroacupuncture frequencies used on maintaining the structural integrity of ischemic brain tissue. Compared with the model and 100 Hz electroacupuncture groups the 15 and 30 Hz electroacupuncture groups displayed decreased neurological deficit scores, as evaluated by the ＂Longa＂ method, significantly increased glial fibrillary acidic protein expression, and alleviated ultrastructural damage of astrocytes at the edge of the infarct. Our experimental findings indicate that 15 and 30 Hz electroacupuncture intervention can favorably maintain the structural integrity of astrocytes and play a protective role in cerebral ischemic injury. Astrocyte structural integrity may be the mechanism underlying acupuncture production of ischemic tolerance
基金ThisprojectwassupportedbyagrantfrominitiativeFoundationofNatioualEducationMinistryforscholarscomingbackfromothercountries (No .2 0 0 1 345)
文摘The involvement of apoptosis in mitochondrial toxin 3 nitropropionic acid (3 NPA) induced ischemic tolerance to transient focal cerebral ischemia in rats and the mechanism was investigated. 3 NPA at a dose of 20 mg/kg or vehicle control was intraperitoneally into the rats. Three days later, rats were exposed to 2 h of middle cerebral artery occlusion followed by 24 h of reperfusion. Infarct volumes were assessed by 2,3,5 triphenyltetrazolinm chloride (TTC) staining 24 h after reperfusion. Neural cell apoptosis in cerebral ischemic penumbra was detected by terminal deoxynucleotidyl transferase mediated dUTP biotin in situ nick end labeling (TUNEL) and flow cytometry methods (FCM). The results showed that as compared to the vehicle treated group, pretreatment with 3 NPA could reduce the infarct volume by 23.3 % and decrease the number of TUNEL positive neural cells and apoptotic percentage by 47 % ( P< 0.05) and 44.9 % ( P< 0 01), respectively. It was concluded that the development of 3 NPA induced ischemic tolerance in brain might be related to the decreases in neural cell apoptosis.
文摘目的 观察局灶性脑缺血预处理对大鼠脑 15 -脂氧化酶 (15 - L O)表达及其 m RNA(15 - L O m RNA)转录的影响 ,探讨两者在诱导脑缺血耐受中的意义。方法 线栓法制备大鼠局灶性脑预缺血 -缺血再灌注模型 (PC-MCAO)及局灶性单纯脑缺血再灌注模型 (MCAO) ,各组于不同时程用免疫组化法检测 15 - L O的表达、原位杂交法检测 15 - L O m RNA的表达。结果 PC- MCAO组较对应时程的 MCAO组 15 - L O m RNA及其蛋白 15 - L O的表达强度显著降低 (P<0 .0 5 )。结论 脑预缺血处理可致 15 - L O及其基因表达下调 ,这可能是局灶性脑缺血预处理诱导脑缺血耐受的机制之一。
基金Supported by grants from National Natural Science Foundation of China(No.31100781)Natural Science Foundation of Hebei Province,China(No.C2011206040).
