目的探讨不同CO2气腹压力对人宫颈癌CaSki细胞体外生长的影响。方法建立CO2气腹体外模型,以不同压力(0、7、14 mm Hg)的CO2气腹对人宫颈癌CaSki细胞处理4 h,设未处理组为对照组。采用MTT法、流式细胞术及电镜比较各组CaSki细胞的生长情...目的探讨不同CO2气腹压力对人宫颈癌CaSki细胞体外生长的影响。方法建立CO2气腹体外模型,以不同压力(0、7、14 mm Hg)的CO2气腹对人宫颈癌CaSki细胞处理4 h,设未处理组为对照组。采用MTT法、流式细胞术及电镜比较各组CaSki细胞的生长情况。结果7 mm Hg组第1-5天吸光度值明显高于0 mm Hg组、14 mm Hg组、对照组,差异有统计学意义(P<0.05);7 mm Hg组G2/M期的人宫颈癌CaSki细胞数值明显高于0 mm Hg组、14 mm Hg组、对照组,差异有统计学意义(P<0.05);14 mm Hg组S期人宫颈癌CaSki细胞数值明显高于0 mm Hg组、7 mm Hg组、对照组,差异有统计学意义(P<0.05);人宫颈癌CaSki细胞的增殖指数随着CO2气腹压力的升高而增高;电镜下观察结果显示,7 mm Hg组、14 mm Hg组均可见明显异常的核分裂像。结论CO2气腹在一定的压力状态下表现出较强的促细胞生长效应。展开更多
Objective To investigate the effect of alpha fetoprotein (AFP) antisense phosphorothioate oligodeoxynucleotides in combination with 5 fluorouracil (5 FU) on the growth of BEL 7404 human hepatoma cells in vitro M...Objective To investigate the effect of alpha fetoprotein (AFP) antisense phosphorothioate oligodeoxynucleotides in combination with 5 fluorouracil (5 FU) on the growth of BEL 7404 human hepatoma cells in vitro Methods Phosphorothioate oligodeoxynucleotides were synthesized by β cyanoethyl phosphoramidite chemistry AFP gene expression in human hepatoma cells was determined by avidin biotin peroxidase complex (ABC) immunocytochemical method Cell growth in the presence or absence of experimental agents was measured using 3 (4, 5 dimethylthiazol 2 yl) 2, 5 dipheny ltetrazolium (MTT) microculture tetrazolium assay Results AFP antisense oligomers markedly suppressed the growth of BEL 7404 human hepatoma cells in vitro by sequence specific blocking of the AFP gene expression in the cells (P<0 05) 5 FU also inhibited the hepatoma cell growth in a dose dependent manner when used alone (P<0 05) The combined treatment with AFP antisense oligomers and 5 FU showed significantly enhanced hepatoma cell growth inhibition than either AFP antisense or 5 FU treated cells alone (P<0 05) Conclusion Combined use of AFP antisense oligomers and 5 FU could more effectively inhibit the growth of BEL 7404 human hepatoma cells in vitro展开更多
Neural stem cells promote neuronal regeneration and repair of brain tissue after injury,but have limited resources and proliferative ability in vivo.We hypothesized that nerve growth factor would promote in vitro prol...Neural stem cells promote neuronal regeneration and repair of brain tissue after injury,but have limited resources and proliferative ability in vivo.We hypothesized that nerve growth factor would promote in vitro proliferation of neural stem cells derived from the tree shrews,a primate-like mammal that has been proposed as an alternative to primates in biomedical translational research.We cultured neural stem cells from the hippocampus of tree shrews at embryonic day 38,and added nerve growth factor（100 μg/L） to the culture medium.Neural stem cells from the hippocampus of tree shrews cultured without nerve growth factor were used as controls.After 3 days,fluorescence microscopy after DAPI and nestin staining revealed that the number of neurospheres and DAPI/nestin-positive cells was markedly greater in the nerve growth factor-treated cells than in control cells.These findings demonstrate that nerve growth factor promotes the proliferation of neural stem cells derived from tree shrews.展开更多
文摘目的探讨不同CO2气腹压力对人宫颈癌CaSki细胞体外生长的影响。方法建立CO2气腹体外模型,以不同压力(0、7、14 mm Hg)的CO2气腹对人宫颈癌CaSki细胞处理4 h,设未处理组为对照组。采用MTT法、流式细胞术及电镜比较各组CaSki细胞的生长情况。结果7 mm Hg组第1-5天吸光度值明显高于0 mm Hg组、14 mm Hg组、对照组,差异有统计学意义(P<0.05);7 mm Hg组G2/M期的人宫颈癌CaSki细胞数值明显高于0 mm Hg组、14 mm Hg组、对照组,差异有统计学意义(P<0.05);14 mm Hg组S期人宫颈癌CaSki细胞数值明显高于0 mm Hg组、7 mm Hg组、对照组,差异有统计学意义(P<0.05);人宫颈癌CaSki细胞的增殖指数随着CO2气腹压力的升高而增高;电镜下观察结果显示,7 mm Hg组、14 mm Hg组均可见明显异常的核分裂像。结论CO2气腹在一定的压力状态下表现出较强的促细胞生长效应。
文摘Objective To investigate the effect of alpha fetoprotein (AFP) antisense phosphorothioate oligodeoxynucleotides in combination with 5 fluorouracil (5 FU) on the growth of BEL 7404 human hepatoma cells in vitro Methods Phosphorothioate oligodeoxynucleotides were synthesized by β cyanoethyl phosphoramidite chemistry AFP gene expression in human hepatoma cells was determined by avidin biotin peroxidase complex (ABC) immunocytochemical method Cell growth in the presence or absence of experimental agents was measured using 3 (4, 5 dimethylthiazol 2 yl) 2, 5 dipheny ltetrazolium (MTT) microculture tetrazolium assay Results AFP antisense oligomers markedly suppressed the growth of BEL 7404 human hepatoma cells in vitro by sequence specific blocking of the AFP gene expression in the cells (P<0 05) 5 FU also inhibited the hepatoma cell growth in a dose dependent manner when used alone (P<0 05) The combined treatment with AFP antisense oligomers and 5 FU showed significantly enhanced hepatoma cell growth inhibition than either AFP antisense or 5 FU treated cells alone (P<0 05) Conclusion Combined use of AFP antisense oligomers and 5 FU could more effectively inhibit the growth of BEL 7404 human hepatoma cells in vitro
基金supported by a grant from the National Key Technology Research and Development Program of the Ministry of Science and Technology of China,No.2014BAI01B00
文摘Neural stem cells promote neuronal regeneration and repair of brain tissue after injury,but have limited resources and proliferative ability in vivo.We hypothesized that nerve growth factor would promote in vitro proliferation of neural stem cells derived from the tree shrews,a primate-like mammal that has been proposed as an alternative to primates in biomedical translational research.We cultured neural stem cells from the hippocampus of tree shrews at embryonic day 38,and added nerve growth factor（100 μg/L） to the culture medium.Neural stem cells from the hippocampus of tree shrews cultured without nerve growth factor were used as controls.After 3 days,fluorescence microscopy after DAPI and nestin staining revealed that the number of neurospheres and DAPI/nestin-positive cells was markedly greater in the nerve growth factor-treated cells than in control cells.These findings demonstrate that nerve growth factor promotes the proliferation of neural stem cells derived from tree shrews.
