BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarco...BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma. METHODS: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed with phase contrast microscope and transmission electron microscope (TEM). Morphometric studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to show tumor-lined channels in human osteosarcoma were also performed. RESULTS: Observation with light microscope and TEM showed that highly aggressive osteosarcoma cell lines (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen, in the absence of endothelial cells or fibroblasts. Morphometric observation using HE stain and CD31 immunohistochemical stain showed that tumor cell-lined channels were also detected in vivo in osteosarcoma; by comparison, all vascular areas in the pedicle of osteochondroma or outside osteochondroma were endothelial-lined. CONCLUSION: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.展开更多
乳腺癌骨转移在复发转移乳腺癌中的发生率为65％～75％。乳腺癌远处转移中，首发症状为骨转移者占27％～50％。骨痛、骨损伤、骨相关事件（skeletal related event．SRE）的发生及生活质量的降低是乳腺癌骨转移的常见并发症。SRE是指在...乳腺癌骨转移在复发转移乳腺癌中的发生率为65％～75％。乳腺癌远处转移中，首发症状为骨转移者占27％～50％。骨痛、骨损伤、骨相关事件（skeletal related event．SRE）的发生及生活质量的降低是乳腺癌骨转移的常见并发症。SRE是指在临床试验中表明双膦酸盐类药物治疗失败的研究观察终点，一般定义为骨痛加剧或出现新的骨痛、病理性骨折、椎体压缩或变形、脊髓压迫、骨放疗、骨转移病灶进展及高钙血症等事件，是影响患者自主活动能力和生活质量的主要因素。展开更多
基金ThisstudywassupportedinpartbyagrantfromtheNationalNaturalScienceFoundationofChina (No 3 0 2 713 14 )
文摘BACKGROUND: Osteosarcoma is characterized by high neovascularization and a high propensity for metastasis through bloodstream. This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma and to illustrate mechanism of tumor blood vessels formation in osteosarcoma. METHODS: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed with phase contrast microscope and transmission electron microscope (TEM). Morphometric studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to show tumor-lined channels in human osteosarcoma were also performed. RESULTS: Observation with light microscope and TEM showed that highly aggressive osteosarcoma cell lines (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen, in the absence of endothelial cells or fibroblasts. Morphometric observation using HE stain and CD31 immunohistochemical stain showed that tumor cell-lined channels were also detected in vivo in osteosarcoma; by comparison, all vascular areas in the pedicle of osteochondroma or outside osteochondroma were endothelial-lined. CONCLUSION: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.
文摘乳腺癌骨转移在复发转移乳腺癌中的发生率为65％～75％。乳腺癌远处转移中，首发症状为骨转移者占27％～50％。骨痛、骨损伤、骨相关事件（skeletal related event．SRE）的发生及生活质量的降低是乳腺癌骨转移的常见并发症。SRE是指在临床试验中表明双膦酸盐类药物治疗失败的研究观察终点，一般定义为骨痛加剧或出现新的骨痛、病理性骨折、椎体压缩或变形、脊髓压迫、骨放疗、骨转移病灶进展及高钙血症等事件，是影响患者自主活动能力和生活质量的主要因素。
