OBJECTIVE:To investigate the effects of Bazhen decoction(BZD),Siwu decoction(SWD) and Sijunzi decoction(SJZD) in mice with anemia induced by5-fluorouracil(5-FU) and discussed the possible pharmacological hematopoietic...OBJECTIVE:To investigate the effects of Bazhen decoction(BZD),Siwu decoction(SWD) and Sijunzi decoction(SJZD) in mice with anemia induced by5-fluorouracil(5-FU) and discussed the possible pharmacological hematopoietic mechanism to provide experimental evidence for the clinical use of the three classical prescriptions in the treatment of anemia.METHODS:Anemia was induced by intravenous injection of 5-FU and 80 female Kunming mice were randomly,assigned to oral administration of SWD,SJZD,or BZD daily for 10 days.Peripheral blood cells count and bone marrow cell cycle were monitored to evaluate anti-anemia effects.Serum cytokines,interferon-γ(IFN-γ),interleukin-3(IL-3),erythropoietin(EPO),granulocyte-macrophage colonystimulating factor(GM-CSF),and tumor necrosis factor-α(TNF-α) were assayed.EPO m RNA expression was assayed in kidney and liver tissue homogenates.RESULTS:BZD and SWD significantly increased the number of red blood cells,hemoglobin concentration,and hematocrit,promoted bone marrow cells to enter the cell cycle,proliferate and differentiate,significantly increased IL-3 secretion,and significantly inhibited IFN-γ secretion.BZD stimulated transcription of EPO m RNA in the kidney and liver and enhanced serum EPO expression.A therapeutic effect of SJZD was not observed.CONCLUSION:BZD and SWD treatment specifically enhanced hematopoietic function and mediated myelopoiesis by altering serum cytokines levels and accelerating entry of bone marrow cells into the cell cycle.Better curative effects were achieved via nourishing both Qi and blood(BZD) than by enriching the blood(SWD) or invigorating Qi(SWZD)alone.展开更多
目的:观察酸枣仁总皂苷对老年阴血亏虚证失眠大鼠脑组织谷氨酸(Glu)、γ-氨基丁酸(GABA)及GABA A受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),模型组(老年阴血亏虚证失眠组,等容生理盐水),阳性对照组(安定0.18 ...目的:观察酸枣仁总皂苷对老年阴血亏虚证失眠大鼠脑组织谷氨酸(Glu)、γ-氨基丁酸(GABA)及GABA A受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),模型组(老年阴血亏虚证失眠组,等容生理盐水),阳性对照组(安定0.18 mg·kg-1·d-1),酸枣仁总皂苷低、高剂量组(15,30 g·kg-1·d-1)。用D-半乳糖致亚急性衰老、环磷酰胺及氢化可的松致阴血亏虚,及自制改良多平台法剥夺快动眼睡眠(REM)48 h,制作阴血亏虚证候失眠老年大鼠模型。造模后各组灌胃给药3周,采用高效液相法检测脑皮质、海马部位Glu,GABA含量;免疫组化染色及RT-PCR法检测脑皮质、海马部位GABA A R的表达变化,并考察酸枣仁总皂苷对这些指标的影响。结果:与空白对照组比较,模型组大鼠脑皮质及海马Glu,GABA含量显著升高(P<0.01,P<0.05),且Glu/GABA增高;脑皮质及海马部位的GABA A R免疫化学累积吸光度明显较高(P<0.01);皮质部位GABA A R mRNA的表达均显著上调(P<0.01),经酸枣仁总皂苷干预后各指标明显下降(P<0.01,P<0.05)。结论:酸枣仁总皂苷治疗阴血亏虚证老年失眠的机制可能与减少脑内氨基酸毒性作用,下调大脑皮质及海马部位GABA A R的表达有关。展开更多
基金Supported by National Training Programs of Innovation for Undergraduates(No.201310635015)
文摘OBJECTIVE:To investigate the effects of Bazhen decoction(BZD),Siwu decoction(SWD) and Sijunzi decoction(SJZD) in mice with anemia induced by5-fluorouracil(5-FU) and discussed the possible pharmacological hematopoietic mechanism to provide experimental evidence for the clinical use of the three classical prescriptions in the treatment of anemia.METHODS:Anemia was induced by intravenous injection of 5-FU and 80 female Kunming mice were randomly,assigned to oral administration of SWD,SJZD,or BZD daily for 10 days.Peripheral blood cells count and bone marrow cell cycle were monitored to evaluate anti-anemia effects.Serum cytokines,interferon-γ(IFN-γ),interleukin-3(IL-3),erythropoietin(EPO),granulocyte-macrophage colonystimulating factor(GM-CSF),and tumor necrosis factor-α(TNF-α) were assayed.EPO m RNA expression was assayed in kidney and liver tissue homogenates.RESULTS:BZD and SWD significantly increased the number of red blood cells,hemoglobin concentration,and hematocrit,promoted bone marrow cells to enter the cell cycle,proliferate and differentiate,significantly increased IL-3 secretion,and significantly inhibited IFN-γ secretion.BZD stimulated transcription of EPO m RNA in the kidney and liver and enhanced serum EPO expression.A therapeutic effect of SJZD was not observed.CONCLUSION:BZD and SWD treatment specifically enhanced hematopoietic function and mediated myelopoiesis by altering serum cytokines levels and accelerating entry of bone marrow cells into the cell cycle.Better curative effects were achieved via nourishing both Qi and blood(BZD) than by enriching the blood(SWD) or invigorating Qi(SWZD)alone.
文摘目的:观察酸枣仁总皂苷对老年阴血亏虚证失眠大鼠脑组织谷氨酸(Glu)、γ-氨基丁酸(GABA)及GABA A受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),模型组(老年阴血亏虚证失眠组,等容生理盐水),阳性对照组(安定0.18 mg·kg-1·d-1),酸枣仁总皂苷低、高剂量组(15,30 g·kg-1·d-1)。用D-半乳糖致亚急性衰老、环磷酰胺及氢化可的松致阴血亏虚,及自制改良多平台法剥夺快动眼睡眠(REM)48 h,制作阴血亏虚证候失眠老年大鼠模型。造模后各组灌胃给药3周,采用高效液相法检测脑皮质、海马部位Glu,GABA含量;免疫组化染色及RT-PCR法检测脑皮质、海马部位GABA A R的表达变化,并考察酸枣仁总皂苷对这些指标的影响。结果:与空白对照组比较,模型组大鼠脑皮质及海马Glu,GABA含量显著升高(P<0.01,P<0.05),且Glu/GABA增高;脑皮质及海马部位的GABA A R免疫化学累积吸光度明显较高(P<0.01);皮质部位GABA A R mRNA的表达均显著上调(P<0.01),经酸枣仁总皂苷干预后各指标明显下降(P<0.01,P<0.05)。结论:酸枣仁总皂苷治疗阴血亏虚证老年失眠的机制可能与减少脑内氨基酸毒性作用,下调大脑皮质及海马部位GABA A R的表达有关。
