目的使用时差培养箱观察比较不同原核持续时间的正常受精胚胎后续发育情况,探讨原核相关动力学参数对胚胎发育潜力的预测价值。方法对2018年9月至2021年1月在衡阳南华星辉生殖健康专科医院行时差成像系统观测的922枚正常受精来源胚胎的...目的使用时差培养箱观察比较不同原核持续时间的正常受精胚胎后续发育情况,探讨原核相关动力学参数对胚胎发育潜力的预测价值。方法对2018年9月至2021年1月在衡阳南华星辉生殖健康专科医院行时差成像系统观测的922枚正常受精来源胚胎的发育情况进行回顾性分析。根据原核持续时间的长短将胚胎分为≤15 h组(n=388)和>15 h组(n=534),比较两组胚胎的优质卵裂胚形成率、囊胚形成率、优质囊胚形成率及临床妊娠率;将行囊胚培养的764枚胚胎分为成囊组(n=558)与未成囊组(n=206),比较两组胚胎的原核持续时间;再根据不同授精方式和原核持续时间分为≤15 h IVF组(n=216)和>15 h IVF组(n=317)、≤15 h ICSI组(n=103)和>15 h ICSI组(n=128)4组,比较各组间胚胎的囊胚形成相关指标。结果以原核持续时间分组分析,>15 h组囊胚形成率(65.8%vs.83.1%)、优质囊胚形成率(35.7%vs.50.8%)和临床妊娠率(55.6%vs.79.6%)均较≤15 h组显著下降(P<0.05),而两组间优质卵裂胚形成率(55.4%vs.59.8%)无统计学差异(P>0.05)。未成囊组胚胎的原核持续时间较成囊组显著延长[(17.85±4.38)h vs.(15.61±2.75)h,P<0.05]。以授精方式+原核持续时间分组分析发现,>15 h IVF组囊胚形成率(68.8%vs.82.9%)、优质囊胚形成率(39.1%vs.50.0%)均较≤15 h IVF组显著下降(P<0.05);>15 h ICSI组囊胚形成率(58.6%vs.83.5%)、优质囊胚形成率(27.3%vs.52.4%)均较≤15 h ICSI组显著下降(P<0.05)。结论无论采用何种授精方式,原核持续时间≤15 h的胚胎囊胚形成率及囊胚质量均显著高于原核持续时间>15 h胚胎。原核持续时间对于胚胎发育潜力有一定的预测价值。展开更多
Objective:To investigate the effect of co-culture with peritoneal macrophage to the fertility rate and blastocyst formation rate of the mouse's oocytes.Methods:A total of 15 KunMing mice aged 10-14 weeks were unde...Objective:To investigate the effect of co-culture with peritoneal macrophage to the fertility rate and blastocyst formation rate of the mouse's oocytes.Methods:A total of 15 KunMing mice aged 10-14 weeks were undergone ovulation induction and oocytes collection.The oocytes were randomly divided into three groups.The first group was the control group and cultured without macrophage;the second group(experimental group 1)was co-cultured with macrophages right after oocytes collection;the third group(experimental group 2)was cultured alone after oocytes retrieval,and co-cultured with macrophages from 8 cell stage.Results:1.The fertility rate in control group(91.0%)and experimental group 2(93.7%)was significantly higher than that in experimental group 1(72.0%,P<0.001).2.Blastocyst formation rate of the fertilized oocytes in the experimental group 2(78.2%)was much higher than control group(60.3%,P<0.001).3.Blastocyst formation rate of the oocytes in experimental group 2(73.2%)was much higher than control group(54.8%)and experimental group 1(50.0%),which were statistically significant.Conclusions:1.Co-culture with peritoneal macrophages had an adverse influence on the fertilization process.2.Peritoneal macrophages could enhance the mouse embryonic development and increase the blastocyst formation rate.展开更多
文摘目的使用时差培养箱观察比较不同原核持续时间的正常受精胚胎后续发育情况,探讨原核相关动力学参数对胚胎发育潜力的预测价值。方法对2018年9月至2021年1月在衡阳南华星辉生殖健康专科医院行时差成像系统观测的922枚正常受精来源胚胎的发育情况进行回顾性分析。根据原核持续时间的长短将胚胎分为≤15 h组(n=388)和>15 h组(n=534),比较两组胚胎的优质卵裂胚形成率、囊胚形成率、优质囊胚形成率及临床妊娠率;将行囊胚培养的764枚胚胎分为成囊组(n=558)与未成囊组(n=206),比较两组胚胎的原核持续时间;再根据不同授精方式和原核持续时间分为≤15 h IVF组(n=216)和>15 h IVF组(n=317)、≤15 h ICSI组(n=103)和>15 h ICSI组(n=128)4组,比较各组间胚胎的囊胚形成相关指标。结果以原核持续时间分组分析,>15 h组囊胚形成率(65.8%vs.83.1%)、优质囊胚形成率(35.7%vs.50.8%)和临床妊娠率(55.6%vs.79.6%)均较≤15 h组显著下降(P<0.05),而两组间优质卵裂胚形成率(55.4%vs.59.8%)无统计学差异(P>0.05)。未成囊组胚胎的原核持续时间较成囊组显著延长[(17.85±4.38)h vs.(15.61±2.75)h,P<0.05]。以授精方式+原核持续时间分组分析发现,>15 h IVF组囊胚形成率(68.8%vs.82.9%)、优质囊胚形成率(39.1%vs.50.0%)均较≤15 h IVF组显著下降(P<0.05);>15 h ICSI组囊胚形成率(58.6%vs.83.5%)、优质囊胚形成率(27.3%vs.52.4%)均较≤15 h ICSI组显著下降(P<0.05)。结论无论采用何种授精方式,原核持续时间≤15 h的胚胎囊胚形成率及囊胚质量均显著高于原核持续时间>15 h胚胎。原核持续时间对于胚胎发育潜力有一定的预测价值。
文摘Objective:To investigate the effect of co-culture with peritoneal macrophage to the fertility rate and blastocyst formation rate of the mouse's oocytes.Methods:A total of 15 KunMing mice aged 10-14 weeks were undergone ovulation induction and oocytes collection.The oocytes were randomly divided into three groups.The first group was the control group and cultured without macrophage;the second group(experimental group 1)was co-cultured with macrophages right after oocytes collection;the third group(experimental group 2)was cultured alone after oocytes retrieval,and co-cultured with macrophages from 8 cell stage.Results:1.The fertility rate in control group(91.0%)and experimental group 2(93.7%)was significantly higher than that in experimental group 1(72.0%,P<0.001).2.Blastocyst formation rate of the fertilized oocytes in the experimental group 2(78.2%)was much higher than control group(60.3%,P<0.001).3.Blastocyst formation rate of the oocytes in experimental group 2(73.2%)was much higher than control group(54.8%)and experimental group 1(50.0%),which were statistically significant.Conclusions:1.Co-culture with peritoneal macrophages had an adverse influence on the fertilization process.2.Peritoneal macrophages could enhance the mouse embryonic development and increase the blastocyst formation rate.
