The thiamethoxam acts as enhancer, allowing maximizing the expression of seed vigor. Isozymes are products of gene expression, highly influenced by the environment, because genes that control its expression are manife...The thiamethoxam acts as enhancer, allowing maximizing the expression of seed vigor. Isozymes are products of gene expression, highly influenced by the environment, because genes that control its expression are manifested in certain stages of development and in specific organs and tissues, or even under the action of certain stimulus. This study evaluated the isoenzyme expression in bean seedlings from seeds treated with thiamethoxam. Bean seeds were used, cultivar Pérola and IAPAR Siriri, submitted or not to drought stress. Seeds were treated at doses of thiamethoxam: 0, 1, 2, 3, 4 and 5 mL·kg-1 of seed. Isozymes were extracted from seedlings collected five days after sowing. We evaluated the expression of isoenzymes: Glutamate Oxalacetate transaminase (GOT), acid phosphatase (FAC), peroxidase (PO), Esterase (EST), malate dehydrogenase (MDH) and glutamate dehydrogenase (GTDH). Interpretation of the results was based on visual analysis of electrophoresis gels, considering the presence/absence and the intensity of each electrophoretic band. Bean seeds treated with thiamethoxam with doses up 3 mL·kg-1 of seed are higher germination untreated, even when submitted to drought stress. The expression of isozymes GOT, FAC, PO, EST, MDH and GTDH is not affected significantly in bean seedlings of Pérola and IPR Siriri under different doses of thiamethoxam with and without drought stress.展开更多
The commonbean (Phaseolus vulgaris L.) is an important agricultural crop due to its nutritional properties. Insecticides and fungicides employed to control pests and diseases in the common bean may cause as yet unknow...The commonbean (Phaseolus vulgaris L.) is an important agricultural crop due to its nutritional properties. Insecticides and fungicides employed to control pests and diseases in the common bean may cause as yet unknown effects on the plant’s morphology and metabolism. Bioactivators are complex organic substances, capable of exerting modification upon growth patterns, plant DNA transcription, gene expression, metabolic enzymes and mineral nutrition. Thiamethoxam insecticide has shown positive action on the enhancement of vigor expression, phytomass accretion and the promotion of deeper root systems. The aim of this work was to evaluate the effect of thiamethoxam on the physiological performance of common bean seeds. Seeds from cultivars Pérola and IAPAR Siriri were treated to thiamethoxam concentrations of 0, 100, 200, 300, 400 and 500 mL·kg-1 seed. Physiological quality was measured through tests for germination value, first count, cold test, accelerated aging and seedling shoot and root lengths, all undertaken in the presence and absence of moisture stress. Thiamethoxam stimulated the physiological performance of common bean seeds under moisture stress or in the absence of it, with levels varying according to cultivar and seed lot. The best physiological seed performance was obtained within the range of 200 mL·kg-1 seed to 400 mL·kg-1 seed.展开更多
High-yielding crops can only achieve their potential when the appropriate technological tools are applied and weather conditions do not impose restrictions. One such technological tool is the treatment of seeds, in wh...High-yielding crops can only achieve their potential when the appropriate technological tools are applied and weather conditions do not impose restrictions. One such technological tool is the treatment of seeds, in which different products are used and, in some cases, cause little-known effects capable of modifying the plant’s metabolism and/or its morphology. The insecticide thiamethoxam alters the plant’s physiology and morphology, accelerating its development, thus enhancing its vigor. The objective of this study was to evaluate the influence of thiamethoxam on the physiological performance of rice seeds during storage. The research was conducted in a green-house and at the laboratory of the Department of Plant Science, Faculty of Agronomy Eliseu Maciel —UFPel (Federal University of Pelotas). Rice seeds from cultivars INIA Olimar and El Paso L144 were treated to a commercial product containing 350 grams of active ingredient thiamethoxam at doses of 0, 100, 200, 300, 400 and 500 g per 100 kg of seed. Seeds were tested for physiological quality immediately after being treated (check treatment) and at 3, 6, 9 and 12 months under storage, through germination test, cold test, accelerated aging and greenhouse emergence test. The concentration range from 200 to 400 g a.i. 100 kg-1 seed yielded the highest germination and seed vigor values for both rice cultivars during the storage period.展开更多
本研究以新疆紫色芜菁与绿色芜菁(Brassica rapa var. rapa)为研究对象,结合超高效液相色谱(UPLC)和串联质(MS/MS)技术,对不同皮色芜菁果肉、果皮中花青苷进行分析鉴定。紫色芜菁与绿色芜菁中含有14种相同的花青苷,不同芜菁品种不同部位...本研究以新疆紫色芜菁与绿色芜菁(Brassica rapa var. rapa)为研究对象,结合超高效液相色谱(UPLC)和串联质(MS/MS)技术,对不同皮色芜菁果肉、果皮中花青苷进行分析鉴定。紫色芜菁与绿色芜菁中含有14种相同的花青苷,不同芜菁品种不同部位,花青苷含量不一致,表皮中含有的花青苷种类较多,果肉中含有的花青苷种类较少。不同皮色的芜菁品种在果肉中均含松香花青素O-己糖苷与松香苷3-O-葡萄糖苷。不同品种芜菁表皮中含有差异显著花青苷代谢物数目为8个,果肉中含有差异显著花青苷代谢物数目为5个;紫色芜菁果肉与绿色芜菁表皮花青素代谢物的相关系数为0.989 7;紫色芜菁表皮与绿色芜菁表皮花青素代谢物的相关系数为0.927 3。本研究完成花青苷代谢物的鉴定分析,明确紫色芜菁块根中花青素的分布,揭示新疆芜菁中花青苷类物质的组成差异与积累特性,这为揭示新疆芜菁中花青苷的合成与积累的分子机制和培育富含花青苷的芜菁新品种提供一定的理论基础。展开更多
文摘The thiamethoxam acts as enhancer, allowing maximizing the expression of seed vigor. Isozymes are products of gene expression, highly influenced by the environment, because genes that control its expression are manifested in certain stages of development and in specific organs and tissues, or even under the action of certain stimulus. This study evaluated the isoenzyme expression in bean seedlings from seeds treated with thiamethoxam. Bean seeds were used, cultivar Pérola and IAPAR Siriri, submitted or not to drought stress. Seeds were treated at doses of thiamethoxam: 0, 1, 2, 3, 4 and 5 mL·kg-1 of seed. Isozymes were extracted from seedlings collected five days after sowing. We evaluated the expression of isoenzymes: Glutamate Oxalacetate transaminase (GOT), acid phosphatase (FAC), peroxidase (PO), Esterase (EST), malate dehydrogenase (MDH) and glutamate dehydrogenase (GTDH). Interpretation of the results was based on visual analysis of electrophoresis gels, considering the presence/absence and the intensity of each electrophoretic band. Bean seeds treated with thiamethoxam with doses up 3 mL·kg-1 of seed are higher germination untreated, even when submitted to drought stress. The expression of isozymes GOT, FAC, PO, EST, MDH and GTDH is not affected significantly in bean seedlings of Pérola and IPR Siriri under different doses of thiamethoxam with and without drought stress.
文摘The commonbean (Phaseolus vulgaris L.) is an important agricultural crop due to its nutritional properties. Insecticides and fungicides employed to control pests and diseases in the common bean may cause as yet unknown effects on the plant’s morphology and metabolism. Bioactivators are complex organic substances, capable of exerting modification upon growth patterns, plant DNA transcription, gene expression, metabolic enzymes and mineral nutrition. Thiamethoxam insecticide has shown positive action on the enhancement of vigor expression, phytomass accretion and the promotion of deeper root systems. The aim of this work was to evaluate the effect of thiamethoxam on the physiological performance of common bean seeds. Seeds from cultivars Pérola and IAPAR Siriri were treated to thiamethoxam concentrations of 0, 100, 200, 300, 400 and 500 mL·kg-1 seed. Physiological quality was measured through tests for germination value, first count, cold test, accelerated aging and seedling shoot and root lengths, all undertaken in the presence and absence of moisture stress. Thiamethoxam stimulated the physiological performance of common bean seeds under moisture stress or in the absence of it, with levels varying according to cultivar and seed lot. The best physiological seed performance was obtained within the range of 200 mL·kg-1 seed to 400 mL·kg-1 seed.
文摘High-yielding crops can only achieve their potential when the appropriate technological tools are applied and weather conditions do not impose restrictions. One such technological tool is the treatment of seeds, in which different products are used and, in some cases, cause little-known effects capable of modifying the plant’s metabolism and/or its morphology. The insecticide thiamethoxam alters the plant’s physiology and morphology, accelerating its development, thus enhancing its vigor. The objective of this study was to evaluate the influence of thiamethoxam on the physiological performance of rice seeds during storage. The research was conducted in a green-house and at the laboratory of the Department of Plant Science, Faculty of Agronomy Eliseu Maciel —UFPel (Federal University of Pelotas). Rice seeds from cultivars INIA Olimar and El Paso L144 were treated to a commercial product containing 350 grams of active ingredient thiamethoxam at doses of 0, 100, 200, 300, 400 and 500 g per 100 kg of seed. Seeds were tested for physiological quality immediately after being treated (check treatment) and at 3, 6, 9 and 12 months under storage, through germination test, cold test, accelerated aging and greenhouse emergence test. The concentration range from 200 to 400 g a.i. 100 kg-1 seed yielded the highest germination and seed vigor values for both rice cultivars during the storage period.
