Anammox is widely considered as the most cost-effective and sustainable process for nitrogen removal.However,how to achieve the enrichment of anammox biomass remains a challenge for its large-scale application,especia...Anammox is widely considered as the most cost-effective and sustainable process for nitrogen removal.However,how to achieve the enrichment of anammox biomass remains a challenge for its large-scale application,especially in mainstream wastewater treatment.In this study,the feasibility of enrichment of anammox biomass was explored through the realization of partial denitrification and the addition of bio-carriers.By using ordinary activated sludge,a sequencing batch reactor(SBR)followed by an up-fow anaerobic sludge bed(UASB)was operated at 25±2℃ for 214 days.The long-term operation was divided into five phases,in which SBR and UASB were started-up in Phases I and II,respectively.By eliminating oxygen and adjusting the infow ratios in Phases III-V,advanced nitrogen removal was achieved with the effuent total nitrogen being 4.7 mg/L and the nitrogen removal efficiency being 90.5%in Phase V.Both in-situ and ex-situ activity tests demonstrated the occurrence of partial denitrification and anammox.Moreover,16S rRNA high-throughput sequencing analysis revealed that Candidatus Brocadia was enriched from below the detection limit to in biofilms(0.4%in SBR,2.2%in UASB)and the foc sludge(0.2%in SBR,1.3%in UASB),while Thauera was mainly detected in the foc sludge(8.1%in SBR,8.8%in UASB),which might play a key role in partial denitrification.Overall,this study provides a novel strategy to enrich anammox biomass driven by rapid achievement of partial denitrification through the addition of bio-carriers,which will improve large-scale application of anammox processes in mainstream wastewater treatment.展开更多
文章以粒径为5 mm的聚乙烯醇(PVA)凝胶颗粒为生物载体,研究其对厌氧污泥颗粒化进程及反应器运行效果的影响。在PVA颗粒污泥初成体形成的情况下,UASB反应器连续运行74 d。试验结果表明:经过32 d的培养,得到沉降性能优良的PVA颗粒污泥,反...文章以粒径为5 mm的聚乙烯醇(PVA)凝胶颗粒为生物载体,研究其对厌氧污泥颗粒化进程及反应器运行效果的影响。在PVA颗粒污泥初成体形成的情况下,UASB反应器连续运行74 d。试验结果表明:经过32 d的培养,得到沉降性能优良的PVA颗粒污泥,反应器COD去除率高达97%,容积负荷由1.05 kg COD·m-3d-1提升至13.33 kg COD·m-3d-1,水力停留时间由35 h缩短至8 h;运行结束时,COD去除率稳定在90%左右,容积负荷达到13.54 kg COD·m-3d-1。通过扫描电镜观察到成熟PVA颗粒污泥外表面丝状菌上交联生长着杆菌、球菌,内部主要附着丝状菌。展开更多
基金supported by the National Natural Science Foundation of China (No.22176007),the Beijing Natural Science Foundation (No.8212022)the fund of innovation and entrepreneurship leading team project in Guangzhou (No.CYLJTD-201607)the National Key Research and Development Program of China (No.2021YFC3200605)。
文摘Anammox is widely considered as the most cost-effective and sustainable process for nitrogen removal.However,how to achieve the enrichment of anammox biomass remains a challenge for its large-scale application,especially in mainstream wastewater treatment.In this study,the feasibility of enrichment of anammox biomass was explored through the realization of partial denitrification and the addition of bio-carriers.By using ordinary activated sludge,a sequencing batch reactor(SBR)followed by an up-fow anaerobic sludge bed(UASB)was operated at 25±2℃ for 214 days.The long-term operation was divided into five phases,in which SBR and UASB were started-up in Phases I and II,respectively.By eliminating oxygen and adjusting the infow ratios in Phases III-V,advanced nitrogen removal was achieved with the effuent total nitrogen being 4.7 mg/L and the nitrogen removal efficiency being 90.5%in Phase V.Both in-situ and ex-situ activity tests demonstrated the occurrence of partial denitrification and anammox.Moreover,16S rRNA high-throughput sequencing analysis revealed that Candidatus Brocadia was enriched from below the detection limit to in biofilms(0.4%in SBR,2.2%in UASB)and the foc sludge(0.2%in SBR,1.3%in UASB),while Thauera was mainly detected in the foc sludge(8.1%in SBR,8.8%in UASB),which might play a key role in partial denitrification.Overall,this study provides a novel strategy to enrich anammox biomass driven by rapid achievement of partial denitrification through the addition of bio-carriers,which will improve large-scale application of anammox processes in mainstream wastewater treatment.
文摘文章以粒径为5 mm的聚乙烯醇(PVA)凝胶颗粒为生物载体,研究其对厌氧污泥颗粒化进程及反应器运行效果的影响。在PVA颗粒污泥初成体形成的情况下,UASB反应器连续运行74 d。试验结果表明:经过32 d的培养,得到沉降性能优良的PVA颗粒污泥,反应器COD去除率高达97%,容积负荷由1.05 kg COD·m-3d-1提升至13.33 kg COD·m-3d-1,水力停留时间由35 h缩短至8 h;运行结束时,COD去除率稳定在90%左右,容积负荷达到13.54 kg COD·m-3d-1。通过扫描电镜观察到成熟PVA颗粒污泥外表面丝状菌上交联生长着杆菌、球菌,内部主要附着丝状菌。
