Changes of catalase(CAT) activities in the liver of Boleophthalmus pectinirostris with benzo(a)pyrene(BaP) exposure are detected in experimental condition. The results show that there is no significant changes for CAT...Changes of catalase(CAT) activities in the liver of Boleophthalmus pectinirostris with benzo(a)pyrene(BaP) exposure are detected in experimental condition. The results show that there is no significant changes for CAT activities in control group and 0.05mg·L -1 BaP group with prolonged exposure ( P >0.05), whereas they are depressed significantly at concentration of 0.5mg·L -1 ( P ≤0.05). In 3 days exposure, CAT activities decrease significantly with the increase of BaP concentration ( P ≤0.05). This might indicate that exposure to higher BaP concentrations might have toxic effect to fishes. The activities of CAT increase significantly to control level after BaP was removed. It turned out that the physiological modulatary mechanism still existed in the liver of Boleophthalmus pectinirostris . All the results show that CAT activity might be suitable to be the bioindicator of BaP exposure.展开更多
提出一种新的方法,用于富集苯并[a]芘耐受菌株。该方法使用多孔介质脱脂棉作为载体,从连续流动的流体——下水道污水中富集菌株。利用介质截留法、富集培养法等理论,为富集目标微生物提供了最佳条件。以苯并[a]芘为唯一碳源和能源,从下...提出一种新的方法,用于富集苯并[a]芘耐受菌株。该方法使用多孔介质脱脂棉作为载体,从连续流动的流体——下水道污水中富集菌株。利用介质截留法、富集培养法等理论,为富集目标微生物提供了最佳条件。以苯并[a]芘为唯一碳源和能源,从下水道沉积物中分离、筛选出4株苯并[a]芘耐受菌株,其中一株能在20天内将40 mg/L的苯并[a]芘降解28.7%。通过16S r RNA基因序列分析和部分生理生化特征分析,鉴定该菌株为Acinetobacter sp.Bap30。这是不动杆菌可降解苯并[a]芘的首次报道。添加其他碳源和低分子量多环芳烃——菲作为共代谢底物,研究菌株的共代谢作用。研究结果对石油污染的土壤或者焦化废水等工业污水中的高分子量多环芳烃——苯并[a]芘具有非常重要的实践意义。展开更多
文摘Changes of catalase(CAT) activities in the liver of Boleophthalmus pectinirostris with benzo(a)pyrene(BaP) exposure are detected in experimental condition. The results show that there is no significant changes for CAT activities in control group and 0.05mg·L -1 BaP group with prolonged exposure ( P >0.05), whereas they are depressed significantly at concentration of 0.5mg·L -1 ( P ≤0.05). In 3 days exposure, CAT activities decrease significantly with the increase of BaP concentration ( P ≤0.05). This might indicate that exposure to higher BaP concentrations might have toxic effect to fishes. The activities of CAT increase significantly to control level after BaP was removed. It turned out that the physiological modulatary mechanism still existed in the liver of Boleophthalmus pectinirostris . All the results show that CAT activity might be suitable to be the bioindicator of BaP exposure.
文摘提出一种新的方法,用于富集苯并[a]芘耐受菌株。该方法使用多孔介质脱脂棉作为载体,从连续流动的流体——下水道污水中富集菌株。利用介质截留法、富集培养法等理论,为富集目标微生物提供了最佳条件。以苯并[a]芘为唯一碳源和能源,从下水道沉积物中分离、筛选出4株苯并[a]芘耐受菌株,其中一株能在20天内将40 mg/L的苯并[a]芘降解28.7%。通过16S r RNA基因序列分析和部分生理生化特征分析,鉴定该菌株为Acinetobacter sp.Bap30。这是不动杆菌可降解苯并[a]芘的首次报道。添加其他碳源和低分子量多环芳烃——菲作为共代谢底物,研究菌株的共代谢作用。研究结果对石油污染的土壤或者焦化废水等工业污水中的高分子量多环芳烃——苯并[a]芘具有非常重要的实践意义。
