目的探讨攻击行为与15个短串联重复序列（ short tandem repeats, STRs） 基因座的关联情况。方法应用AmpFlSTR Identifiler^TM荧光标记复合扩增体系对541例攻击行为者（研究组）与459名非攻击行为个体（对照组）的15个STR基因座（CSF1P...目的探讨攻击行为与15个短串联重复序列（ short tandem repeats, STRs） 基因座的关联情况。方法应用AmpFlSTR Identifiler^TM荧光标记复合扩增体系对541例攻击行为者（研究组）与459名非攻击行为个体（对照组）的15个STR基因座（CSF1PO、D2S1338、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D19S433、D21S11、FGA、TH01、TPOX、vWA）进行聚合酶链反应复合扩增，然后应用ABI3130XL型基因分析系统对扩增产物进行电泳和基因检测，观察两组15个STR基因座等位基因及基因型频率的差异。结果15个STR基因座中，D2S1338、D19S433基因座的等位基因及基因型频率在两组间的分布差异有统计学意义（P〈0．01）；单因素分析显示，D2S1338基因座等位基因16及基因型19-22、22—24和D19S433基因座基因型13—14．2的频率分布在两组间的差异均有统计学意义（P=0．0018、P=0．0001、P=0．0003、P=0．0000），OR值分别为7．380（95％CI：1．701～32．028）、0．051（95％CI：0．007～0．388）、13．933（95％CI：1．845～105．717）、0．349（95％CI：0．216～0．564）；其他STR基因座等位基因频率及基因型频率在两组间的差异均无统计学意义（P〉0．05）。结论D2S1338和D19S433基因座与攻击行为的发生可能相关联；D2S1338基因座等位基因16、基因型22—24可能为攻击行为发生的易感因素，D2S1338基因座的基因型19—22和D19S433基因座基因型13—14．2可能为攻击行为发生的抗性因素。展开更多
Detailed aspects of the transition from the solitarious to the gregarious phase in the framework of locust ecology are undoubtedly most important for understanding locust phase polyphenism. Nevertheless, due to obviou...Detailed aspects of the transition from the solitarious to the gregarious phase in the framework of locust ecology are undoubtedly most important for understanding locust phase polyphenism. Nevertheless, due to obvious difficulties in studying the solitarious phase in nature, such information is limited and mostly available from research carried out under laboratory conditions. In the current study, we examined the dispersal patterns of newly hatched locust nymphs in a laboratory setup that simulated seminatural conditions. This was carried out with no previous manipulation of the nymphs other than controlling their parental density. We comparatively tested the spatial distribution of newly hatched nymphs on perches located at different ranges within an emergence arena, and the expected Poisson （random） distribution. Hatchlings were found to disperse among the perches in a pattern significantly different from that expected by random. Irrespective of their parents＇ phase, the observed distributions of all nymphs were clearly clumped, similar or close to those expected for gregarious locusts. It seems that rather than emerging with a parentally derived and predetermined phase, hatchlings have an independent default or innate behavioral state, which reflects at least tolerance if not attraction to conspecifics. The typical phase behavior may later become dominant under the appropriate environmental conditions. These results imply novel perspectives on locust phase transformation, which contribute to our understanding of the formation of locust crowds under field conditions. These should be considered in any rationale for developing a preventative management strategy of locust populations.展开更多
目的探讨精神分裂症攻击行为与短串联重复序列（short tandem repeats，STR）基因座的关联情况。方法应用PowerPlex 21 System荧光标记复合扩增体系对123例有攻击行为的精神分裂症患者与489例无攻击行为的精神分裂症患者的20个STR基因座...目的探讨精神分裂症攻击行为与短串联重复序列（short tandem repeats，STR）基因座的关联情况。方法应用PowerPlex 21 System荧光标记复合扩增体系对123例有攻击行为的精神分裂症患者与489例无攻击行为的精神分裂症患者的20个STR基因座（D3S1368、D1S1656、D6S1043、D13S317、Penta E、D16S639、D18S51、D2S1338、CSF1PO、PentaD、TH01、vWA、D21S11、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433、FGA）进行聚合酶链反应复合扩增，应用AB3500xL型基因分析系统对扩增产物进行基因分型，观察2组基因座等位基因及基因型频率的差异。结果20个STR基因座均符合Hardy-Weinberg定律（P〉0．05）；PentaD基因座等位基因及基因型频率分布在两组间的差异有统计学意义（等位基因：P=0．042；基因型：P=0．014），其余19个STR基因座等位基因和基因型频率分布在两组间的差异无统计学意义（P〉0．05）；单因素分析显示，PentaD基因座等位基因10和基因型10-12的频率分布在两组间的差异均有统计学意义（P=0．0027，P=0．0001），OR值分别为1．81（95％CI：1．22-2．67）和4．33（95％CI：1．95-9．59）。结论PentaD基因座多态性与精神分裂症攻击行为的发生可能相关联；PentaD基因座等位基因10和基因型10-12可能为精神分裂症攻击行为的发生的易感因素。展开更多
文摘目的探讨攻击行为与15个短串联重复序列（ short tandem repeats, STRs） 基因座的关联情况。方法应用AmpFlSTR Identifiler^TM荧光标记复合扩增体系对541例攻击行为者（研究组）与459名非攻击行为个体（对照组）的15个STR基因座（CSF1PO、D2S1338、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D19S433、D21S11、FGA、TH01、TPOX、vWA）进行聚合酶链反应复合扩增，然后应用ABI3130XL型基因分析系统对扩增产物进行电泳和基因检测，观察两组15个STR基因座等位基因及基因型频率的差异。结果15个STR基因座中，D2S1338、D19S433基因座的等位基因及基因型频率在两组间的分布差异有统计学意义（P〈0．01）；单因素分析显示，D2S1338基因座等位基因16及基因型19-22、22—24和D19S433基因座基因型13—14．2的频率分布在两组间的差异均有统计学意义（P=0．0018、P=0．0001、P=0．0003、P=0．0000），OR值分别为7．380（95％CI：1．701～32．028）、0．051（95％CI：0．007～0．388）、13．933（95％CI：1．845～105．717）、0．349（95％CI：0．216～0．564）；其他STR基因座等位基因频率及基因型频率在两组间的差异均无统计学意义（P〉0．05）。结论D2S1338和D19S433基因座与攻击行为的发生可能相关联；D2S1338基因座等位基因16、基因型22—24可能为攻击行为发生的易感因素，D2S1338基因座的基因型19—22和D19S433基因座基因型13—14．2可能为攻击行为发生的抗性因素。
文摘Detailed aspects of the transition from the solitarious to the gregarious phase in the framework of locust ecology are undoubtedly most important for understanding locust phase polyphenism. Nevertheless, due to obvious difficulties in studying the solitarious phase in nature, such information is limited and mostly available from research carried out under laboratory conditions. In the current study, we examined the dispersal patterns of newly hatched locust nymphs in a laboratory setup that simulated seminatural conditions. This was carried out with no previous manipulation of the nymphs other than controlling their parental density. We comparatively tested the spatial distribution of newly hatched nymphs on perches located at different ranges within an emergence arena, and the expected Poisson （random） distribution. Hatchlings were found to disperse among the perches in a pattern significantly different from that expected by random. Irrespective of their parents＇ phase, the observed distributions of all nymphs were clearly clumped, similar or close to those expected for gregarious locusts. It seems that rather than emerging with a parentally derived and predetermined phase, hatchlings have an independent default or innate behavioral state, which reflects at least tolerance if not attraction to conspecifics. The typical phase behavior may later become dominant under the appropriate environmental conditions. These results imply novel perspectives on locust phase transformation, which contribute to our understanding of the formation of locust crowds under field conditions. These should be considered in any rationale for developing a preventative management strategy of locust populations.
