A new rapid and sensitive high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of atorvastatin—an antihyperlipidemic drug along with most commonly prescribed drug...A new rapid and sensitive high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of atorvastatin—an antihyperlipidemic drug along with most commonly prescribed drugs (antihyperlipidemic, antihypertensive, antidiabetic, antithrombotic) in bulk and marketed combined formulations. The chromatographic separation was carried out by gradient elution mode with acetonitrile as organic modifier and 0.1% triethylamine acetate (TEAA) buffer pH 5 at a flow rate of 1 mL/min and a diode array detector at wavelength 230 nm was employed for detection of the analytes. Calibration curves were linear in the range of 5-150 mg/mL for all the drugs with correlation coefficients of determination (r 2 values)Z0.999. Limits of detection (LODs) and Limits of quantification (LOQs) ranged from 0.1 to 0.27 mg/mL and 0.3 to 0.89 mg/mL respectively. Intra-day and inter-day precision was studied at three concentration levels (20, 60 and 100 mg/mL). The intra-day and inter-day RSD for all compounds was less than 2.0%. The accuracy for all compounds was found to be between 98% and 102%. Thus, the performance of the method described allows its use in quantification of atorvastatin along with 9 most commonly prescribed drugs available in market as atorvastatin combined dosage forms.展开更多
A novel three-dimensional holographic vector of atomic interaction field（3D-HoVAIF） was used to describe the chemical structures of 23 benzoxazinone derivatives as antithrombotic drugs.Here a quantitative structure ...A novel three-dimensional holographic vector of atomic interaction field（3D-HoVAIF） was used to describe the chemical structures of 23 benzoxazinone derivatives as antithrombotic drugs.Here a quantitative structure activity relationship（QSAR） model was built by partial least-squares（PLS） regression.The estimation stability and prediction ability of the model were strictly analyzed by both internal and external validations.The correlation coefficients of established PLS model,leave-one-out（LOO） cross-validation,and predicted values versus experimental ones of external samples were R2=0.899,RCV2=0.854 and Qext2=0.868,respectively.These values indicated that the built PLS model had both favorable estimation stability and good prediction capabilities.Furthermore,the satisfactory results showed that 3D-HoVAIF could preferably express the information related to the biological activity of benzoxazinone derivatives.展开更多
目的系统评价经皮冠状动脉介入术(PCI)患者应用替格瑞洛或氯吡格雷抗血栓的有效性和安全性,为临床医师对PCI患者的抗血栓药物品种选择提供循证参考。方法计算机检索万方数据库、CNKI、Web of Science、PubMed、Medline、Embase、Cochran...目的系统评价经皮冠状动脉介入术(PCI)患者应用替格瑞洛或氯吡格雷抗血栓的有效性和安全性,为临床医师对PCI患者的抗血栓药物品种选择提供循证参考。方法计算机检索万方数据库、CNKI、Web of Science、PubMed、Medline、Embase、Cochrane Library,收集替格瑞洛(试验组)和氯吡格雷(对照组)随机对照试验(RCT)的相关研究文献。采用Cochrane手册5.1.0版的RCT偏倚风险评估工具对纳入文献质量进行评价,采用Rev Man 5.3和stata 15.0软件进行meta统计分析。结果共纳入16项RCT。Meta分析结果显示,在有效性方面,试验组全因性死亡[RR=0.83,95%CI(0.75~0.91),P=0.0001]、中风事件[RR=0.84,95%CI(0.71~0.98),P=0.03]、心肌梗死事件[RR=0.79,95%CI(0.72~0.87),P<0.00001]、心脏不良事件[RR=0.69,95%CI(0.50~0.97),P=0.03]、支架血栓事件[RR=0.61,95%CI(0.42~0.89),P=0.01]发生风险均明显低于对照组;在安全性方面,试验组总出血事件[RR=1.14,95%CI(1.01~1.29),P=0.03]、大出血事件[RR=1.32,95%CI(1.09~1.60),P=0.004]、轻微出血事件[RR=1.35,95%CI(1.09~1.66),P=0.005]发生风险却高于对照组,呼吸困难事件[RR=1.71,95%CI(0.97~3.03),P=0.07]及心动过缓事件[RR=1.04,95%CI(0.83~1.32),P=0.72],两组差异无统计学意义。结论替格瑞洛较氯吡格雷在PCI术后患者抗血栓治疗的有效性方面较优,但安全性方面相对较差。展开更多
基金the Department of Pharmaceuticals (Ministry of Chemicals and Fertilizers),New Delhi,for grant of a MS(Pharm.) fellowship
文摘A new rapid and sensitive high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of atorvastatin—an antihyperlipidemic drug along with most commonly prescribed drugs (antihyperlipidemic, antihypertensive, antidiabetic, antithrombotic) in bulk and marketed combined formulations. The chromatographic separation was carried out by gradient elution mode with acetonitrile as organic modifier and 0.1% triethylamine acetate (TEAA) buffer pH 5 at a flow rate of 1 mL/min and a diode array detector at wavelength 230 nm was employed for detection of the analytes. Calibration curves were linear in the range of 5-150 mg/mL for all the drugs with correlation coefficients of determination (r 2 values)Z0.999. Limits of detection (LODs) and Limits of quantification (LOQs) ranged from 0.1 to 0.27 mg/mL and 0.3 to 0.89 mg/mL respectively. Intra-day and inter-day precision was studied at three concentration levels (20, 60 and 100 mg/mL). The intra-day and inter-day RSD for all compounds was less than 2.0%. The accuracy for all compounds was found to be between 98% and 102%. Thus, the performance of the method described allows its use in quantification of atorvastatin along with 9 most commonly prescribed drugs available in market as atorvastatin combined dosage forms.
基金supported by the Natural Science Foundation of Shaanxi Province (2009JQ2005)Foundation of Educational Commission of Shaanxi Province (09JK358) Graduate Innovation Fund of Shaanxi University of Science and Technology
文摘A novel three-dimensional holographic vector of atomic interaction field（3D-HoVAIF） was used to describe the chemical structures of 23 benzoxazinone derivatives as antithrombotic drugs.Here a quantitative structure activity relationship（QSAR） model was built by partial least-squares（PLS） regression.The estimation stability and prediction ability of the model were strictly analyzed by both internal and external validations.The correlation coefficients of established PLS model,leave-one-out（LOO） cross-validation,and predicted values versus experimental ones of external samples were R2=0.899,RCV2=0.854 and Qext2=0.868,respectively.These values indicated that the built PLS model had both favorable estimation stability and good prediction capabilities.Furthermore,the satisfactory results showed that 3D-HoVAIF could preferably express the information related to the biological activity of benzoxazinone derivatives.
文摘目的系统评价经皮冠状动脉介入术(PCI)患者应用替格瑞洛或氯吡格雷抗血栓的有效性和安全性,为临床医师对PCI患者的抗血栓药物品种选择提供循证参考。方法计算机检索万方数据库、CNKI、Web of Science、PubMed、Medline、Embase、Cochrane Library,收集替格瑞洛(试验组)和氯吡格雷(对照组)随机对照试验(RCT)的相关研究文献。采用Cochrane手册5.1.0版的RCT偏倚风险评估工具对纳入文献质量进行评价,采用Rev Man 5.3和stata 15.0软件进行meta统计分析。结果共纳入16项RCT。Meta分析结果显示,在有效性方面,试验组全因性死亡[RR=0.83,95%CI(0.75~0.91),P=0.0001]、中风事件[RR=0.84,95%CI(0.71~0.98),P=0.03]、心肌梗死事件[RR=0.79,95%CI(0.72~0.87),P<0.00001]、心脏不良事件[RR=0.69,95%CI(0.50~0.97),P=0.03]、支架血栓事件[RR=0.61,95%CI(0.42~0.89),P=0.01]发生风险均明显低于对照组;在安全性方面,试验组总出血事件[RR=1.14,95%CI(1.01~1.29),P=0.03]、大出血事件[RR=1.32,95%CI(1.09~1.60),P=0.004]、轻微出血事件[RR=1.35,95%CI(1.09~1.66),P=0.005]发生风险却高于对照组,呼吸困难事件[RR=1.71,95%CI(0.97~3.03),P=0.07]及心动过缓事件[RR=1.04,95%CI(0.83~1.32),P=0.72],两组差异无统计学意义。结论替格瑞洛较氯吡格雷在PCI术后患者抗血栓治疗的有效性方面较优,但安全性方面相对较差。
