In the present work, four trace metals, V, Ni, Cu and Fe, have been determined in five crude oil samples of Eastern Baghdad area, Iraq by flame atomic absorption spectrophotometry. The crude oils are characterized wit...In the present work, four trace metals, V, Ni, Cu and Fe, have been determined in five crude oil samples of Eastern Baghdad area, Iraq by flame atomic absorption spectrophotometry. The crude oils are characterized with API gravity values in the range, 25 - 15. The V/Ni ratios for the studied crudes were in the range 3.7 to 5.4 and indicated a typical of the sapropelic-type organic matter. The Ni and V contents were correlated with API gravity where an increase of metal contents with the decrease in the API values could be observed. The plots of V and Ni versus sulfur indicated linear correlation. This is attributed to the increase of porphyrinic structures of the metals. The method of direct dilution (DD) with organic solvent and the standard addition (SA) were evaluated as compared with standard dry ashing-acid dissolution (DA). In most cases the DD method gave V and Ni concentrations that are higher than DA and for the heavier crude oil (API = 15) the DD results were significantly smaller than those of the DA method. The SA method can be a good solution to determine the trace metals in heavy crude oils provided that the addition must not exceed the levels of the metal concentration in the diluted samples.展开更多
文中提出将油田井口三相流检测数据上传云服务器的设计方案,定义了原始数据上传格式,编写服务器端解析程序的同时利用传输控制协议(transmission control protocol, TCP)上传数据,将得到的原始数据经过模型优化处理,计算油田需求的实际...文中提出将油田井口三相流检测数据上传云服务器的设计方案,定义了原始数据上传格式,编写服务器端解析程序的同时利用传输控制协议(transmission control protocol, TCP)上传数据,将得到的原始数据经过模型优化处理,计算油田需求的实际参数并存入服务器数据库中,供石油工程师在线提取及分析。通过多次试验测试,数据库存储稳定有序,验证了三相流测量仪器与服务器互连的可行性。文中井口三相流检测系统的设计可大量收集一手数据,也降低了油田工人手动测量的偶然误差,并通过大数据分析预测未来油井的生产状况。在石油仪器科技领域有广泛应用。展开更多
目的：探讨寻常性银屑病患者皮损及外周血miRNA表达谱系，寻找表达一致的miRNA。方法用基因芯片技术筛选17例银屑病皮损和外周血miRNA，通过验证筛选出差异性miRNA，探讨其与银屑病皮损和面积严重度指数（PASI）的相关性。结果利用Agile...目的：探讨寻常性银屑病患者皮损及外周血miRNA表达谱系，寻找表达一致的miRNA。方法用基因芯片技术筛选17例银屑病皮损和外周血miRNA，通过验证筛选出差异性miRNA，探讨其与银屑病皮损和面积严重度指数（PASI）的相关性。结果利用Agilent Human miRNA芯片技术,得出银屑病皮损和外周血中相一致的15个miRNA，经过RT-qPCR技术验证，表达一致的miRNA有7个，其中miR-30e-5p、miR-192-5p、miR-17-3p、miR-1227-5p的表达量与银屑病患者PASI评分呈负相关（P＜0.05）；miR-125b-5p、miR-642a-5p、miR-29a-5p与PASI评分无明显相关性（P＞0.05）。结论寻常性银屑病患者皮损组织和血浆中存在着表达一致的miRNA，这些miRNA有望作为评估银屑病患者病情严重程度的生物标记物之一。展开更多
目的:探讨荧光原位杂交技术(fluorescence in situ hybridization,FISH)在产前诊断染色体嵌合体中的应用。方法:22例行绒毛/羊水细胞体外培养染色体核型结果提示可能存在染色体嵌合现象的病例,进一步用染色体微阵列比较基因组杂交检测(c...目的:探讨荧光原位杂交技术(fluorescence in situ hybridization,FISH)在产前诊断染色体嵌合体中的应用。方法:22例行绒毛/羊水细胞体外培养染色体核型结果提示可能存在染色体嵌合现象的病例,进一步用染色体微阵列比较基因组杂交检测(chromosomal microarray analaysis,CMA)和FISH技术分析确定其异常核型及嵌合比例。结果:22例核型结果提示嵌合体的病例中,性染色体数目嵌合13例,常染色体三体嵌合6例,性染色体结构异常嵌合3例。近50%病例CMA结果未检测到嵌合现象,可能与CMA技术仅能检测基因拷贝数变化且无法检测低比例嵌合有关。因常染色体数目异常对胎儿生长发育影响较大,常染色体数目异常嵌合病例中,除1例病例外其余均选择终止妊娠。3例结构异常嵌合病例经核型分析结合CMA及FISH结果确定均为性染色体双着丝粒染色体复杂结构嵌合。结论:对于产前诊断提示可能存在染色体嵌合的病例,不应急于终止妊娠,需采用多种方法加以验证,应充分应用CMA技术和FISH技术结合G显带核型结果进行综合分析,并需要对胎儿形态进行细致的超声评估,从而更加准确地确定嵌合类型以及异常核型的嵌合比例,为临床医师指导遗传咨询提供更加可靠的依据。展开更多
文摘In the present work, four trace metals, V, Ni, Cu and Fe, have been determined in five crude oil samples of Eastern Baghdad area, Iraq by flame atomic absorption spectrophotometry. The crude oils are characterized with API gravity values in the range, 25 - 15. The V/Ni ratios for the studied crudes were in the range 3.7 to 5.4 and indicated a typical of the sapropelic-type organic matter. The Ni and V contents were correlated with API gravity where an increase of metal contents with the decrease in the API values could be observed. The plots of V and Ni versus sulfur indicated linear correlation. This is attributed to the increase of porphyrinic structures of the metals. The method of direct dilution (DD) with organic solvent and the standard addition (SA) were evaluated as compared with standard dry ashing-acid dissolution (DA). In most cases the DD method gave V and Ni concentrations that are higher than DA and for the heavier crude oil (API = 15) the DD results were significantly smaller than those of the DA method. The SA method can be a good solution to determine the trace metals in heavy crude oils provided that the addition must not exceed the levels of the metal concentration in the diluted samples.
文摘文中提出将油田井口三相流检测数据上传云服务器的设计方案,定义了原始数据上传格式,编写服务器端解析程序的同时利用传输控制协议(transmission control protocol, TCP)上传数据,将得到的原始数据经过模型优化处理,计算油田需求的实际参数并存入服务器数据库中,供石油工程师在线提取及分析。通过多次试验测试,数据库存储稳定有序,验证了三相流测量仪器与服务器互连的可行性。文中井口三相流检测系统的设计可大量收集一手数据,也降低了油田工人手动测量的偶然误差,并通过大数据分析预测未来油井的生产状况。在石油仪器科技领域有广泛应用。
文摘目的：探讨寻常性银屑病患者皮损及外周血miRNA表达谱系，寻找表达一致的miRNA。方法用基因芯片技术筛选17例银屑病皮损和外周血miRNA，通过验证筛选出差异性miRNA，探讨其与银屑病皮损和面积严重度指数（PASI）的相关性。结果利用Agilent Human miRNA芯片技术,得出银屑病皮损和外周血中相一致的15个miRNA，经过RT-qPCR技术验证，表达一致的miRNA有7个，其中miR-30e-5p、miR-192-5p、miR-17-3p、miR-1227-5p的表达量与银屑病患者PASI评分呈负相关（P＜0.05）；miR-125b-5p、miR-642a-5p、miR-29a-5p与PASI评分无明显相关性（P＞0.05）。结论寻常性银屑病患者皮损组织和血浆中存在着表达一致的miRNA，这些miRNA有望作为评估银屑病患者病情严重程度的生物标记物之一。
