This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal o...This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.展开更多
光纤光栅信号的探测电路设计是实现物理量高精度检测的重要环节。针对高频、高灵敏度、高信噪比的检测需求,利用负向偏置的光电二极管和运算放大器设计了光电转换电路,滤波电路采用压控电压源二阶低通巴特沃斯型滤波器,频率响应范围为0~...光纤光栅信号的探测电路设计是实现物理量高精度检测的重要环节。针对高频、高灵敏度、高信噪比的检测需求,利用负向偏置的光电二极管和运算放大器设计了光电转换电路,滤波电路采用压控电压源二阶低通巴特沃斯型滤波器,频率响应范围为0~200 k Hz。实验结果表明,电路对静态和动态冲击信号都有较好的检测效果且保持较高的信噪比,为进一步信号的分析和处理提供了良好的前提。展开更多
We have developed a two-stage Ti：sapphire amplifier system which can produce 17- TW/23-fs pulses at a repetition rate 10Hz. A birefringent plate is used in the regenerative amplifier to alleviate gain narrowing, whil...We have developed a two-stage Ti：sapphire amplifier system which can produce 17- TW/23-fs pulses at a repetition rate 10Hz. A birefringent plate is used in the regenerative amplifier to alleviate gain narrowing, while an all- reflective cylindrical-mirror-based pulse stretcher and an acousto-optic programmable dispersive filter （AOPDF） are used to compensate for the higher order dispersion of the system.展开更多
针对目前在线运行的CPC(center position control)自动对中控制系统存在响应速度慢、容易受到现场干扰以及检测精度低的问题,设计开发了一种新的高性能CPC自动对中控制系统;该系统主要是采用电磁感应原理检测带钢的位置,然后采用电子电...针对目前在线运行的CPC(center position control)自动对中控制系统存在响应速度慢、容易受到现场干扰以及检测精度低的问题,设计开发了一种新的高性能CPC自动对中控制系统;该系统主要是采用电磁感应原理检测带钢的位置,然后采用电子电路对控制对象的位置信号进行放大、滤波,再经过STM32微控制器、控制柜、液压控制等单元调整导辊位置来实现对控制对象的对中控制;整个电路系统的检测和控制精度为1mm,并且信号线性度以及稳定性好,提高了产品的可靠性。展开更多
基金supported by the National Supporting Plan of China (2011BAZ03288, 2012BAI23B00)the National Program on Key Basic Research Projects of China (2011CB707701)+2 种基金the National Natural Science Foundation of China (60978064)the Science Plan Foundation of Zhejiang (2012C31G3210006)the TSINGHUA-SIEMENS Collective Foundation (20103000273)
文摘This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.
文摘光纤光栅信号的探测电路设计是实现物理量高精度检测的重要环节。针对高频、高灵敏度、高信噪比的检测需求,利用负向偏置的光电二极管和运算放大器设计了光电转换电路,滤波电路采用压控电压源二阶低通巴特沃斯型滤波器,频率响应范围为0~200 k Hz。实验结果表明,电路对静态和动态冲击信号都有较好的检测效果且保持较高的信噪比,为进一步信号的分析和处理提供了良好的前提。
文摘We have developed a two-stage Ti：sapphire amplifier system which can produce 17- TW/23-fs pulses at a repetition rate 10Hz. A birefringent plate is used in the regenerative amplifier to alleviate gain narrowing, while an all- reflective cylindrical-mirror-based pulse stretcher and an acousto-optic programmable dispersive filter （AOPDF） are used to compensate for the higher order dispersion of the system.
文摘针对目前在线运行的CPC(center position control)自动对中控制系统存在响应速度慢、容易受到现场干扰以及检测精度低的问题,设计开发了一种新的高性能CPC自动对中控制系统;该系统主要是采用电磁感应原理检测带钢的位置,然后采用电子电路对控制对象的位置信号进行放大、滤波,再经过STM32微控制器、控制柜、液压控制等单元调整导辊位置来实现对控制对象的对中控制;整个电路系统的检测和控制精度为1mm,并且信号线性度以及稳定性好,提高了产品的可靠性。
